OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen
The mass mortality of molluscs caused by OsHV-1 infection has frequently occurred worldwide in recent years. Meanwhile the interaction between OsHV-1 and its host is largely unknown. Innate immunity mainly makes up the mollusc defense system, due to the lack of adaptive immunity in invertebrates. Th...
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ROYAL SOC CHEMISTRY
2019
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Online Access: | http://ir.qdio.ac.cn/handle/337002/161414 https://doi.org/10.1039/c9mt00018f |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/161414 2023-05-15T15:59:00+02:00 OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen Xin, Lusheng Huang, Bowen Zhang, Huan Li, Chen Bai, Changming Wang, Chongming 2019-04-01 http://ir.qdio.ac.cn/handle/337002/161414 https://doi.org/10.1039/c9mt00018f 英语 eng ROYAL SOC CHEMISTRY METALLOMICS http://ir.qdio.ac.cn/handle/337002/161414 doi:10.1039/c9mt00018f Biochemistry & Molecular Biology CRASSOSTREA-GIGAS PACIFIC OYSTERS TOMALES BAY MU-VAR VIRUS HERPESVIRUS IDENTIFICATION MORTALITIES CALIFORNIA 期刊论文 2019 ftchinacasciocas https://doi.org/10.1039/c9mt00018f 2022-06-27T05:40:44Z The mass mortality of molluscs caused by OsHV-1 infection has frequently occurred worldwide in recent years. Meanwhile the interaction between OsHV-1 and its host is largely unknown. Innate immunity mainly makes up the mollusc defense system, due to the lack of adaptive immunity in invertebrates. The iron limitation strategy is an indispensable facet of innate immunity across vertebrate and invertebrate species. In this study, an iron limitation strategy was interestingly found to contribute to mollusc innate immune responses against OsHV-1 infection. Firstly, ark clams, Scapharca broughtonii, were experimentally infected with OsHV-1, and serious hyperaemia in hepatopancreases and the erosion of gills were observed post OsHV-1 infection according to a histology assay. Meanwhile, based on quantification and Prussian blue staining, the process of iron efflux from ark clams was described post OsHV-1 infection. Secondly, ferritin, as an important iron storage protein, was characterized in ark clams and showed significant iron binding activity. According to the results of an immunohistochemistry assay, ferritin was supposed to be responsible for the iron translocation in ark clams post OsHV-1 infection. Its expression level was significantly fluctuant in response to OsHV-1 infection. Finally, oxidative stress was assessed by the analyses of H2O2 content, total antioxidant capacity and MDA level post OsHV-1 infection. Supplementary iron was found to promote ROS generation and death of hemocytes in vivo. These results highlighted that microenvironment changes in the essential nutrient iron should be an important aspect of the pathogenesis of OsHV-1 disease. Report Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Metallomics 11 4 822 832 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Biochemistry & Molecular Biology CRASSOSTREA-GIGAS PACIFIC OYSTERS TOMALES BAY MU-VAR VIRUS HERPESVIRUS IDENTIFICATION MORTALITIES CALIFORNIA |
spellingShingle |
Biochemistry & Molecular Biology CRASSOSTREA-GIGAS PACIFIC OYSTERS TOMALES BAY MU-VAR VIRUS HERPESVIRUS IDENTIFICATION MORTALITIES CALIFORNIA Xin, Lusheng Huang, Bowen Zhang, Huan Li, Chen Bai, Changming Wang, Chongming OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen |
topic_facet |
Biochemistry & Molecular Biology CRASSOSTREA-GIGAS PACIFIC OYSTERS TOMALES BAY MU-VAR VIRUS HERPESVIRUS IDENTIFICATION MORTALITIES CALIFORNIA |
description |
The mass mortality of molluscs caused by OsHV-1 infection has frequently occurred worldwide in recent years. Meanwhile the interaction between OsHV-1 and its host is largely unknown. Innate immunity mainly makes up the mollusc defense system, due to the lack of adaptive immunity in invertebrates. The iron limitation strategy is an indispensable facet of innate immunity across vertebrate and invertebrate species. In this study, an iron limitation strategy was interestingly found to contribute to mollusc innate immune responses against OsHV-1 infection. Firstly, ark clams, Scapharca broughtonii, were experimentally infected with OsHV-1, and serious hyperaemia in hepatopancreases and the erosion of gills were observed post OsHV-1 infection according to a histology assay. Meanwhile, based on quantification and Prussian blue staining, the process of iron efflux from ark clams was described post OsHV-1 infection. Secondly, ferritin, as an important iron storage protein, was characterized in ark clams and showed significant iron binding activity. According to the results of an immunohistochemistry assay, ferritin was supposed to be responsible for the iron translocation in ark clams post OsHV-1 infection. Its expression level was significantly fluctuant in response to OsHV-1 infection. Finally, oxidative stress was assessed by the analyses of H2O2 content, total antioxidant capacity and MDA level post OsHV-1 infection. Supplementary iron was found to promote ROS generation and death of hemocytes in vivo. These results highlighted that microenvironment changes in the essential nutrient iron should be an important aspect of the pathogenesis of OsHV-1 disease. |
format |
Report |
author |
Xin, Lusheng Huang, Bowen Zhang, Huan Li, Chen Bai, Changming Wang, Chongming |
author_facet |
Xin, Lusheng Huang, Bowen Zhang, Huan Li, Chen Bai, Changming Wang, Chongming |
author_sort |
Xin, Lusheng |
title |
OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen |
title_short |
OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen |
title_full |
OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen |
title_fullStr |
OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen |
title_full_unstemmed |
OsHV-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen |
title_sort |
oshv-1 infection leads to mollusc tissue lesion and iron redistribution, revealing a strategy of iron limitation against pathogen |
publisher |
ROYAL SOC CHEMISTRY |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/161414 https://doi.org/10.1039/c9mt00018f |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
METALLOMICS http://ir.qdio.ac.cn/handle/337002/161414 doi:10.1039/c9mt00018f |
op_doi |
https://doi.org/10.1039/c9mt00018f |
container_title |
Metallomics |
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11 |
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4 |
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822 |
op_container_end_page |
832 |
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1766394786854469632 |