Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure
Antioxidant enzymes play essential roles against oxidative stress caused by 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), which is ubiquitous in marine environment and organisms. However, research on antioxidant responses to BDE-47 in marine bivalves is scarce. In this study, we identified...
Published in: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
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Main Authors: | , , , , , , , , , , , , , |
Format: | Report |
Language: | English |
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ELSEVIER SCIENCE INC
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Online Access: | http://ir.qdio.ac.cn/handle/337002/161145 https://doi.org/10.1016/j.cbpc.2018.12.003 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/161145 2023-05-15T17:54:21+02:00 Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure Dong, Shihang Yang, Yanyan Cheng, Bo Ren, Chuanbo Zhang, Huawei Xu, Hua Zhu, Na Wang, Wansheng Dang, Yongjian Li, Mingzhu Chen, Jun Wang, Kai Zhang, Libin Fang, Yan 2019-03-01 http://ir.qdio.ac.cn/handle/337002/161145 https://doi.org/10.1016/j.cbpc.2018.12.003 英语 eng ELSEVIER SCIENCE INC COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY http://ir.qdio.ac.cn/handle/337002/161145 doi:10.1016/j.cbpc.2018.12.003 Catalase Glutathione peroxidase Superoxide dismutase 2,2 ',4,4 '-Tetrabromodiphenyl ether Malondialdehyde Mactra veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology POLYBROMINATED DIPHENYL ETHERS MESSENGER-RNA EXPRESSION MUSSEL WATCH PROGRAM SUPEROXIDE-DISMUTASE OXIDATIVE STRESS MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING DIGESTIVE GLAND ENZYME-ACTIVITY PACIFIC OYSTER 期刊论文 2019 ftchinacasciocas https://doi.org/10.1016/j.cbpc.2018.12.003 2022-06-27T05:40:38Z Antioxidant enzymes play essential roles against oxidative stress caused by 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), which is ubiquitous in marine environment and organisms. However, research on antioxidant responses to BDE-47 in marine bivalves is scarce. In this study, we identified the full-length cDNA of catalase (CAT), and glutathione peroxidase (GPx) in the clam Mactra veneriformis. Subsequently, the responses of CAT, GPx, and copper, zinc-superoxide dismutase (Cu, Zn-SOD) were investigated in the clams exposed to 0.1, 1, and 10 mu g/L BDE-47 for 7 days, and then depurated in natural seawater for 3 days. MvCAT and MvGPx contained conserved sequences. The deduced amino acid sequences shared high similarity with CATs and GPxs in other mollusks. M. veneriformis accumulated BDE-47 in a dose-dependent manner and eliminated BDE-47 poorly. BDE-47 induced a time- and dose-dependent increase of malondialdehyde content. Both the dose and the duration had significant effect on mRNA expressions and activities of the three antioxidants. Cu, Zn-SOD responded to BDE-47 earlier than CAT and GPx. The antioxidant responses could recover after depuration. These results suggested that M. veneriformis could accumulate BDE-47 efficiently. Antioxidant enzymes were triggered to counter the oxidative stress generated by BDE-47. Cu, Zn-SOD acted as the first defense against oxidative stress, while CAT and GPx intervened later. This study is therefore helpful in understanding the antioxidant responses to PBDEs in marine bivalves. Report Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 217 98 105 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Catalase Glutathione peroxidase Superoxide dismutase 2,2 ',4,4 '-Tetrabromodiphenyl ether Malondialdehyde Mactra veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology POLYBROMINATED DIPHENYL ETHERS MESSENGER-RNA EXPRESSION MUSSEL WATCH PROGRAM SUPEROXIDE-DISMUTASE OXIDATIVE STRESS MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING DIGESTIVE GLAND ENZYME-ACTIVITY PACIFIC OYSTER |
spellingShingle |
Catalase Glutathione peroxidase Superoxide dismutase 2,2 ',4,4 '-Tetrabromodiphenyl ether Malondialdehyde Mactra veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology POLYBROMINATED DIPHENYL ETHERS MESSENGER-RNA EXPRESSION MUSSEL WATCH PROGRAM SUPEROXIDE-DISMUTASE OXIDATIVE STRESS MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING DIGESTIVE GLAND ENZYME-ACTIVITY PACIFIC OYSTER Dong, Shihang Yang, Yanyan Cheng, Bo Ren, Chuanbo Zhang, Huawei Xu, Hua Zhu, Na Wang, Wansheng Dang, Yongjian Li, Mingzhu Chen, Jun Wang, Kai Zhang, Libin Fang, Yan Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure |
topic_facet |
Catalase Glutathione peroxidase Superoxide dismutase 2,2 ',4,4 '-Tetrabromodiphenyl ether Malondialdehyde Mactra veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology POLYBROMINATED DIPHENYL ETHERS MESSENGER-RNA EXPRESSION MUSSEL WATCH PROGRAM SUPEROXIDE-DISMUTASE OXIDATIVE STRESS MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING DIGESTIVE GLAND ENZYME-ACTIVITY PACIFIC OYSTER |
description |
Antioxidant enzymes play essential roles against oxidative stress caused by 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), which is ubiquitous in marine environment and organisms. However, research on antioxidant responses to BDE-47 in marine bivalves is scarce. In this study, we identified the full-length cDNA of catalase (CAT), and glutathione peroxidase (GPx) in the clam Mactra veneriformis. Subsequently, the responses of CAT, GPx, and copper, zinc-superoxide dismutase (Cu, Zn-SOD) were investigated in the clams exposed to 0.1, 1, and 10 mu g/L BDE-47 for 7 days, and then depurated in natural seawater for 3 days. MvCAT and MvGPx contained conserved sequences. The deduced amino acid sequences shared high similarity with CATs and GPxs in other mollusks. M. veneriformis accumulated BDE-47 in a dose-dependent manner and eliminated BDE-47 poorly. BDE-47 induced a time- and dose-dependent increase of malondialdehyde content. Both the dose and the duration had significant effect on mRNA expressions and activities of the three antioxidants. Cu, Zn-SOD responded to BDE-47 earlier than CAT and GPx. The antioxidant responses could recover after depuration. These results suggested that M. veneriformis could accumulate BDE-47 efficiently. Antioxidant enzymes were triggered to counter the oxidative stress generated by BDE-47. Cu, Zn-SOD acted as the first defense against oxidative stress, while CAT and GPx intervened later. This study is therefore helpful in understanding the antioxidant responses to PBDEs in marine bivalves. |
format |
Report |
author |
Dong, Shihang Yang, Yanyan Cheng, Bo Ren, Chuanbo Zhang, Huawei Xu, Hua Zhu, Na Wang, Wansheng Dang, Yongjian Li, Mingzhu Chen, Jun Wang, Kai Zhang, Libin Fang, Yan |
author_facet |
Dong, Shihang Yang, Yanyan Cheng, Bo Ren, Chuanbo Zhang, Huawei Xu, Hua Zhu, Na Wang, Wansheng Dang, Yongjian Li, Mingzhu Chen, Jun Wang, Kai Zhang, Libin Fang, Yan |
author_sort |
Dong, Shihang |
title |
Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure |
title_short |
Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure |
title_full |
Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure |
title_fullStr |
Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure |
title_full_unstemmed |
Responses of antioxidant defenses in the clam Mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure |
title_sort |
responses of antioxidant defenses in the clam mactra veneriformis to 2,2 ',4, 4 '-tetrabromodiphenyl ether exposure |
publisher |
ELSEVIER SCIENCE INC |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/161145 https://doi.org/10.1016/j.cbpc.2018.12.003 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY http://ir.qdio.ac.cn/handle/337002/161145 doi:10.1016/j.cbpc.2018.12.003 |
op_doi |
https://doi.org/10.1016/j.cbpc.2018.12.003 |
container_title |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
container_volume |
217 |
container_start_page |
98 |
op_container_end_page |
105 |
_version_ |
1766162098404982784 |