The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas
Integrins are an important family of cell receptors that can bind foreign particles and promote cell phagocytosis after they are activated. In the present study, a novel beta integrin was identified from pacific oyster Crassostrea gigas with an extracellular domain, a single transmembrane segment, a...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2019
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Online Access: | http://ir.qdio.ac.cn/handle/337002/161137 http://ir.qdio.ac.cn/handle/337002/161138 https://doi.org/10.1016/j.fsi.2019.01.047 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/161138 2023-05-15T15:58:32+02:00 The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas Lv, Zhao Qiu, Limei Jia, Zhihao Wang, Weilin Liu, Zhaoqun Wang, Lingling Song, Linsheng 2019-04-01 http://ir.qdio.ac.cn/handle/337002/161137 http://ir.qdio.ac.cn/handle/337002/161138 https://doi.org/10.1016/j.fsi.2019.01.047 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/161137 http://ir.qdio.ac.cn/handle/337002/161138 doi:10.1016/j.fsi.2019.01.047 C. gigas RGD-Binding Integrin activation Integrin-mediated phagocytosis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences 期刊论文 2019 ftchinacasciocas https://doi.org/10.1016/j.fsi.2019.01.047 2022-07-29T12:11:37Z Integrins are an important family of cell receptors that can bind foreign particles and promote cell phagocytosis after they are activated. In the present study, a novel beta integrin was identified from pacific oyster Crassostrea gigas with an extracellular domain, a single transmembrane segment, and a short cytoplasmic domain. It was phylogenetically clustered with phagocytosis-related insecta beta V, and designated as Cg beta V. Cg beta V shared a conserved NPX[Y/F] motif related to integrin activation with other phagocytosis-related beta integrins. The mRNA transcripts of Cg beta V were widely detected in oyster tissues including hemocytes, gonad, adductor muscle, mantle, gill, and hepatopancreas, and the expression level in hemocytes was significantly up-regulated at 12 h after lipopolysaccharide (LPS) stimulation (p < 0.05), which was 2.29-fold higher than that in the control group. Cg beta V proteins were mainly observed on the hemocytes surface. The oyster hemocytes were found to bind fluorescein isothiocyanate (FITC)-labeled Arg-Gly-Asp-containing peptides (RGDCPs), and the binding capability was significantly up-regulated with the peak percentage of 37.6% at 12 h post LPS stimulation, which was higher than that in the control group (8.8%, p < 0.05), suggesting the activation of RGD-binding integrins on oyster hemocytes surface. The label-free RGDCPs and anti-Cg beta V antibody inhibited the binding capability of hemocytes towards FITC-labeled RGDCPs, which were significant lower in RGD blocking group (7.4%, p < 0.05) and anti-Cg beta V blocking group (22.1%, p < 0.05) than that in the control group (37.6%), indicating that Cg beta V could be a RGD-binding integrin. Phagocytosis assay demonstrated that LPS could enhance the phagocytosis of hemocytes towards Escherichia cob and fluorescent beads with the phagocytic rate (PR) of 18.3% and 17.4%, and phagocytic index (PI) of 5.29 and 37.71, respectively, which were significant higher than that in the control group (11.0% and 3.65 for E. coli, ... Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Fish & Shellfish Immunology 87 638 649 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
C. gigas RGD-Binding Integrin activation Integrin-mediated phagocytosis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
C. gigas RGD-Binding Integrin activation Integrin-mediated phagocytosis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Lv, Zhao Qiu, Limei Jia, Zhihao Wang, Weilin Liu, Zhaoqun Wang, Lingling Song, Linsheng The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas |
topic_facet |
C. gigas RGD-Binding Integrin activation Integrin-mediated phagocytosis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Integrins are an important family of cell receptors that can bind foreign particles and promote cell phagocytosis after they are activated. In the present study, a novel beta integrin was identified from pacific oyster Crassostrea gigas with an extracellular domain, a single transmembrane segment, and a short cytoplasmic domain. It was phylogenetically clustered with phagocytosis-related insecta beta V, and designated as Cg beta V. Cg beta V shared a conserved NPX[Y/F] motif related to integrin activation with other phagocytosis-related beta integrins. The mRNA transcripts of Cg beta V were widely detected in oyster tissues including hemocytes, gonad, adductor muscle, mantle, gill, and hepatopancreas, and the expression level in hemocytes was significantly up-regulated at 12 h after lipopolysaccharide (LPS) stimulation (p < 0.05), which was 2.29-fold higher than that in the control group. Cg beta V proteins were mainly observed on the hemocytes surface. The oyster hemocytes were found to bind fluorescein isothiocyanate (FITC)-labeled Arg-Gly-Asp-containing peptides (RGDCPs), and the binding capability was significantly up-regulated with the peak percentage of 37.6% at 12 h post LPS stimulation, which was higher than that in the control group (8.8%, p < 0.05), suggesting the activation of RGD-binding integrins on oyster hemocytes surface. The label-free RGDCPs and anti-Cg beta V antibody inhibited the binding capability of hemocytes towards FITC-labeled RGDCPs, which were significant lower in RGD blocking group (7.4%, p < 0.05) and anti-Cg beta V blocking group (22.1%, p < 0.05) than that in the control group (37.6%), indicating that Cg beta V could be a RGD-binding integrin. Phagocytosis assay demonstrated that LPS could enhance the phagocytosis of hemocytes towards Escherichia cob and fluorescent beads with the phagocytic rate (PR) of 18.3% and 17.4%, and phagocytic index (PI) of 5.29 and 37.71, respectively, which were significant higher than that in the control group (11.0% and 3.65 for E. coli, ... |
format |
Report |
author |
Lv, Zhao Qiu, Limei Jia, Zhihao Wang, Weilin Liu, Zhaoqun Wang, Lingling Song, Linsheng |
author_facet |
Lv, Zhao Qiu, Limei Jia, Zhihao Wang, Weilin Liu, Zhaoqun Wang, Lingling Song, Linsheng |
author_sort |
Lv, Zhao |
title |
The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas |
title_short |
The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas |
title_full |
The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas |
title_fullStr |
The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas |
title_full_unstemmed |
The activated beta-integrin (Cg beta V) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas |
title_sort |
activated beta-integrin (cg beta v) enhances rgd-binding and phagocytic capabilities of hemocytes in crassostrea gigas |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/161137 http://ir.qdio.ac.cn/handle/337002/161138 https://doi.org/10.1016/j.fsi.2019.01.047 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/161137 http://ir.qdio.ac.cn/handle/337002/161138 doi:10.1016/j.fsi.2019.01.047 |
op_doi |
https://doi.org/10.1016/j.fsi.2019.01.047 |
container_title |
Fish & Shellfish Immunology |
container_volume |
87 |
container_start_page |
638 |
op_container_end_page |
649 |
_version_ |
1766394292428865536 |