A novel LRR and Ig domain-containing protein could function as an immune effector in Crassostrea gigas

A variety of combinations of leucine-rich repeat (LRR) and immunoglobulin-like (Ig) domains have been found and discovered in invertebrates and vertebrates, but the functions remain largely unexplored. In the present study, a novel LRR and Ig domain-containing protein (LRRIG), CgLRRIG-3, was identif...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Wang, Xiudan, Zhao, Xiaoli, Yan, Chunyu, Jia, Zhihao, Lv, Zhao, Ma, Cuiping, Wang, Mengqiang
Format: Report
Language:English
Published: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 2019
Subjects:
Crassostrea gigas
Leucine-rich repeat domain
Immunoglobulin domain
LRRIG
Pattern recognition receptor
Immune effector
Fisheries
Immunology
Marine & Freshwater Biology
Veterinary Sciences
LEUCINE-RICH REPEAT
SOLUBLE ADENYLYL-CYCLASE
ONLY PROTEINS
RECOGNITION
SUPERFAMILY
EXPRESSION
PATTERN
PHAGOCYTOSIS
MECHANISMS
COMPLEXITY
Psi
Online Access:http://ir.qdio.ac.cn/handle/337002/161103
https://doi.org/10.1016/j.fsi.2019.03.003
Description
Summary:A variety of combinations of leucine-rich repeat (LRR) and immunoglobulin-like (Ig) domains have been found and discovered in invertebrates and vertebrates, but the functions remain largely unexplored. In the present study, a novel LRR and Ig domain-containing protein (LRRIG), CgLRRIG-3, was identified and characterized from oyster Crassostrea gigas. It contained two typical LRR motifs, a LRRNT motif and an Ig domain and PSI-BALST and phylogeny analysis revealed that the sequence of CgLRRIG-3 was most related with leucine-rich repeat neuronal 1 proteins from vertebrate. Its mRNA transcripts were constitutively expressed in muscle, gill, hepatopancreas, mantle, gonad and hemocytes with the highest level in hepatopancreas. The mRNA expression level of CgLRRIG-3 in hemocytes could respond to the stimulations of variety PAMPs including lipopolysaccharide (LPS), peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C). The recombinant proteins exhibited a wide PAMP binding repertoire to four typical PAMPs and could significantly induce the expression of CgTNF-1 and CgIL17-5 as well as increase phagocytosis in primary cultured oyster hemocytes. In hepatopancreas, CgLRRIG-3 was mainly distributed in the basolateral membrane of digestive tubule and the hemocoel sinusoid between the digestive tubules. And in hemocytes, the positive signal was mainly distributed in a special group of granulocytes. These results collectively indicated that CgLRRIG-3 could not only function as an immune effector.

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