Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)
Egg yolk generation is a common physiological process in oviparous animals. To understand oogenesis and reproductive capacity, it is necessary to characterize vitellogenins (Vtgs), which are the precursors of major egg yolk proteins (Yps). Therefore, to improve our understanding of the entire proces...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Language: | English |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/160460 http://ir.qdio.ac.cn/handle/337002/160461 https://doi.org/10.1016/j.cbpb.2018.08.002 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/160461 2023-05-15T18:15:42+02:00 Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) Xue, Rui Wang, Xueying Xu, Shihong Liu, Yifan Feng, Chengcheng Zhao, Chunyan Liu, Qinghua Li, Jun 2018-12-01 http://ir.qdio.ac.cn/handle/337002/160460 http://ir.qdio.ac.cn/handle/337002/160461 https://doi.org/10.1016/j.cbpb.2018.08.002 英语 eng ELSEVIER SCIENCE INC COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY http://ir.qdio.ac.cn/handle/337002/160460 http://ir.qdio.ac.cn/handle/337002/160461 doi:10.1016/j.cbpb.2018.08.002 Autosynthesis Estradiol-17 beta Turbot (Scophthalmus maximus) Vitellogenin Yolk protein Biochemistry & Molecular Biology Zoology 期刊论文 2018 ftchinacasciocas https://doi.org/10.1016/j.cbpb.2018.08.002 2022-06-27T05:40:26Z Egg yolk generation is a common physiological process in oviparous animals. To understand oogenesis and reproductive capacity, it is necessary to characterize vitellogenins (Vtgs), which are the precursors of major egg yolk proteins (Yps). Therefore, to improve our understanding of the entire process of egg yolk generation in female turbot (Scophthalmus maximus), we obtained full-length cDNAs of vtg genes, examined gene expression in the female liver and ovary, and analyzed Vtg synthesis in the ovary. Three distinct complete polypeptide sequences were identified and designated as VtgAa, VtgAb, and VtgC, which confirmed the multiplicity of the vtg gene in turbot and showed that it follows a "three vtg model". The expression of these three vtg genes in the female liver was far higher than that in other tissues, including the ovary. The expression of all three vtg genes was extremely low before vitellogenesis, and then increased and was maintained at a high level until the degradation stage, which was in accordance with changes in the concentration of estradiol-17 beta (E-2) and the gonadosomatic index. Compared with the liver, the ovary had a higher E-2 level and lower vtg expression, suggesting that some other factors limit high vtg expression in the ovary of turbot. Transcripts of vtgAb and the Yps derived from them were both detected in oogonia and primary oocytes, which showed that these might possess the ability to perform autosynthesis of yolk. These findings add to our understanding of the reproductive physiology of Vtg synthesis in turbot. Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 226 53 63 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Autosynthesis Estradiol-17 beta Turbot (Scophthalmus maximus) Vitellogenin Yolk protein Biochemistry & Molecular Biology Zoology |
spellingShingle |
Autosynthesis Estradiol-17 beta Turbot (Scophthalmus maximus) Vitellogenin Yolk protein Biochemistry & Molecular Biology Zoology Xue, Rui Wang, Xueying Xu, Shihong Liu, Yifan Feng, Chengcheng Zhao, Chunyan Liu, Qinghua Li, Jun Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) |
topic_facet |
Autosynthesis Estradiol-17 beta Turbot (Scophthalmus maximus) Vitellogenin Yolk protein Biochemistry & Molecular Biology Zoology |
description |
Egg yolk generation is a common physiological process in oviparous animals. To understand oogenesis and reproductive capacity, it is necessary to characterize vitellogenins (Vtgs), which are the precursors of major egg yolk proteins (Yps). Therefore, to improve our understanding of the entire process of egg yolk generation in female turbot (Scophthalmus maximus), we obtained full-length cDNAs of vtg genes, examined gene expression in the female liver and ovary, and analyzed Vtg synthesis in the ovary. Three distinct complete polypeptide sequences were identified and designated as VtgAa, VtgAb, and VtgC, which confirmed the multiplicity of the vtg gene in turbot and showed that it follows a "three vtg model". The expression of these three vtg genes in the female liver was far higher than that in other tissues, including the ovary. The expression of all three vtg genes was extremely low before vitellogenesis, and then increased and was maintained at a high level until the degradation stage, which was in accordance with changes in the concentration of estradiol-17 beta (E-2) and the gonadosomatic index. Compared with the liver, the ovary had a higher E-2 level and lower vtg expression, suggesting that some other factors limit high vtg expression in the ovary of turbot. Transcripts of vtgAb and the Yps derived from them were both detected in oogonia and primary oocytes, which showed that these might possess the ability to perform autosynthesis of yolk. These findings add to our understanding of the reproductive physiology of Vtg synthesis in turbot. |
format |
Report |
author |
Xue, Rui Wang, Xueying Xu, Shihong Liu, Yifan Feng, Chengcheng Zhao, Chunyan Liu, Qinghua Li, Jun |
author_facet |
Xue, Rui Wang, Xueying Xu, Shihong Liu, Yifan Feng, Chengcheng Zhao, Chunyan Liu, Qinghua Li, Jun |
author_sort |
Xue, Rui |
title |
Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) |
title_short |
Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) |
title_full |
Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) |
title_fullStr |
Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) |
title_full_unstemmed |
Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus) |
title_sort |
expression profile and localization of vitellogenin mrna and protein during ovarian development in turbot (scophthalmus maximus) |
publisher |
ELSEVIER SCIENCE INC |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/160460 http://ir.qdio.ac.cn/handle/337002/160461 https://doi.org/10.1016/j.cbpb.2018.08.002 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY http://ir.qdio.ac.cn/handle/337002/160460 http://ir.qdio.ac.cn/handle/337002/160461 doi:10.1016/j.cbpb.2018.08.002 |
op_doi |
https://doi.org/10.1016/j.cbpb.2018.08.002 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
226 |
container_start_page |
53 |
op_container_end_page |
63 |
_version_ |
1766188881352327168 |