Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda
Edwardsiella tarda is one of the leading fish pathogens for the aquaculture industry. To realize efficient disease control of edwardsiellosis, a predictive model for E. tarda in seawater was developed. The modified logistic model was used to regress the growth curves of E. tarda JN at five different...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/160215 2023-05-15T18:41:09+02:00 Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda Fu, Songzhe Tu, Junling Rahman, Md Mostafizur Tian, Huiqin Xiao, Peng Liu, Ying 2018-10-01 http://ir.qdio.ac.cn/handle/337002/160214 http://ir.qdio.ac.cn/handle/337002/160215 https://doi.org/10.1007/s13213-018-1371-x 英语 eng SPRINGER ANNALS OF MICROBIOLOGY http://ir.qdio.ac.cn/handle/337002/160214 http://ir.qdio.ac.cn/handle/337002/160215 doi:10.1007/s13213-018-1371-x Edwardsiella tarda Growth rate Lag time Predictive model Saturation-growth rate model RAS Biotechnology & Applied Microbiology Microbiology 期刊论文 2018 ftchinacasciocas https://doi.org/10.1007/s13213-018-1371-x 2022-06-27T05:40:20Z Edwardsiella tarda is one of the leading fish pathogens for the aquaculture industry. To realize efficient disease control of edwardsiellosis, a predictive model for E. tarda in seawater was developed. The modified logistic model was used to regress the growth curves of E. tarda JN at five different temperatures (range from 10 to 30 degrees C) and four organic nutrient concentrations (range from 5 to 40mgl(-1) measured by chemical oxygen demand (COD)). The modeling effects of temperature and COD on the specific growth rate () were developed by square-root model and saturation-growth rate model, respectively. The growth model was validated in turbot aquaculture tanks by estimating the dynamics of inoculated E. tarda. The accurate feeding of probiotic Bacillus pumilus strain H2 was calculated based on the estimation of E. tarda. Results showed that the logistic model produced a good fit to the growth curves of E. tarda JN (average R-2=0.962). The overall predictions based on above models agreed well with the growth curve of E. tarda JN observed by plate counting in the validation tests (average Af=1.16; average Bf=1.32). The use of predicted amount of B. pumilus (5.66logCFUml(-1)) successfully prevent the deterioration of disease for turbot with 13.3% mortality rate in a recirculating aquaculture system (RAS), while the feeding of 0 and 3.0logCFUml(-1) of B. pumilus resulted in 53.7 and 75.3% of turbot mortality rate, respectively. In conclusion, accurate estimation of E. tarda realized the precise feeding of probiotics, which successfully prevent the rapid progression of the edwardsiellosis. Report Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Annals of Microbiology 68 10 645 654 |
institution |
Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Edwardsiella tarda Growth rate Lag time Predictive model Saturation-growth rate model RAS Biotechnology & Applied Microbiology Microbiology |
spellingShingle |
Edwardsiella tarda Growth rate Lag time Predictive model Saturation-growth rate model RAS Biotechnology & Applied Microbiology Microbiology Fu, Songzhe Tu, Junling Rahman, Md Mostafizur Tian, Huiqin Xiao, Peng Liu, Ying Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda |
topic_facet |
Edwardsiella tarda Growth rate Lag time Predictive model Saturation-growth rate model RAS Biotechnology & Applied Microbiology Microbiology |
description |
Edwardsiella tarda is one of the leading fish pathogens for the aquaculture industry. To realize efficient disease control of edwardsiellosis, a predictive model for E. tarda in seawater was developed. The modified logistic model was used to regress the growth curves of E. tarda JN at five different temperatures (range from 10 to 30 degrees C) and four organic nutrient concentrations (range from 5 to 40mgl(-1) measured by chemical oxygen demand (COD)). The modeling effects of temperature and COD on the specific growth rate () were developed by square-root model and saturation-growth rate model, respectively. The growth model was validated in turbot aquaculture tanks by estimating the dynamics of inoculated E. tarda. The accurate feeding of probiotic Bacillus pumilus strain H2 was calculated based on the estimation of E. tarda. Results showed that the logistic model produced a good fit to the growth curves of E. tarda JN (average R-2=0.962). The overall predictions based on above models agreed well with the growth curve of E. tarda JN observed by plate counting in the validation tests (average Af=1.16; average Bf=1.32). The use of predicted amount of B. pumilus (5.66logCFUml(-1)) successfully prevent the deterioration of disease for turbot with 13.3% mortality rate in a recirculating aquaculture system (RAS), while the feeding of 0 and 3.0logCFUml(-1) of B. pumilus resulted in 53.7 and 75.3% of turbot mortality rate, respectively. In conclusion, accurate estimation of E. tarda realized the precise feeding of probiotics, which successfully prevent the rapid progression of the edwardsiellosis. |
format |
Report |
author |
Fu, Songzhe Tu, Junling Rahman, Md Mostafizur Tian, Huiqin Xiao, Peng Liu, Ying |
author_facet |
Fu, Songzhe Tu, Junling Rahman, Md Mostafizur Tian, Huiqin Xiao, Peng Liu, Ying |
author_sort |
Fu, Songzhe |
title |
Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda |
title_short |
Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda |
title_full |
Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda |
title_fullStr |
Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda |
title_full_unstemmed |
Precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of Edwardsiella tarda |
title_sort |
precise feeding of probiotics in the treatment of edwardsiellosis by accurate estimation of edwardsiella tarda |
publisher |
SPRINGER |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/160214 http://ir.qdio.ac.cn/handle/337002/160215 https://doi.org/10.1007/s13213-018-1371-x |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
ANNALS OF MICROBIOLOGY http://ir.qdio.ac.cn/handle/337002/160214 http://ir.qdio.ac.cn/handle/337002/160215 doi:10.1007/s13213-018-1371-x |
op_doi |
https://doi.org/10.1007/s13213-018-1371-x |
container_title |
Annals of Microbiology |
container_volume |
68 |
container_issue |
10 |
container_start_page |
645 |
op_container_end_page |
654 |
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1766230643760431104 |