An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development
Though the roles of BMP signaling in development is studied extensively in insects and vertebrates, our knowledge of BMP signaling in molluscan development is limited. In the present study, we performed a genome-based analysis of TGF-beta receptors in the Pacific oyster Crassostrea gigas and reveale...
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Online Access: | http://ir.qdio.ac.cn/handle/337002/159371 http://ir.qdio.ac.cn/handle/337002/159372 https://doi.org/10.1016/j.gene.2018.04.035 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/159372 2023-05-15T15:58:54+02:00 An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development Tan, Sujian Huan, Pin Liu, Baozhong 2018-07-15 http://ir.qdio.ac.cn/handle/337002/159371 http://ir.qdio.ac.cn/handle/337002/159372 https://doi.org/10.1016/j.gene.2018.04.035 英语 eng ELSEVIER SCIENCE BV GENE http://ir.qdio.ac.cn/handle/337002/159371 http://ir.qdio.ac.cn/handle/337002/159372 doi:10.1016/j.gene.2018.04.035 BMP signaling Dorsomorphin Dorsal-ventral patterning Shell field Mollusc Genetics & Heredity 期刊论文 2018 ftchinacasciocas https://doi.org/10.1016/j.gene.2018.04.035 2022-06-27T05:40:03Z Though the roles of BMP signaling in development is studied extensively in insects and vertebrates, our knowledge of BMP signaling in molluscan development is limited. In the present study, we performed a genome-based analysis of TGF-beta receptors in the Pacific oyster Crassostrea gigas and revealed that C. gigas possessed all five canonical members of the gene family, including three type I and two type II receptors. Whole mount in situ hybridization revealed that four receptor genes exhibited universal expression at the gastrula stage but cgi-bmprll mRNA was only expressed at the anterior lip of blastopore, indicating the regulation of BMP signaling through restricted expression of TGF-beta receptors. Treatment of oyster embryos using a BMP signaling inhibitor (dorsomorphin) resulted in obvious changes of the expression of developmental regulatory genes. In particular, three dorsally expressed genes were inhibited and two genes expressed at the opposite side showed increased expression, indicating BMP signaling may function in dorsal-ventral (DV) patterning. Some of the influenced genes were potential shell-formation (pSF) genes, including the well-accepted pSF gene engrailed, and thus this indicates the development of shell field was affected by dorsomorphin treatment. Subsequent scanning electronic observation revealed morphological change of the posterior margin of the shell field. Taken together, these results indicate the roles of BMP signaling in both DV patterning and development of shell field in oyster embryo, which expands our knowledge of early molluscan development. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Gene 663 65 71 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
BMP signaling Dorsomorphin Dorsal-ventral patterning Shell field Mollusc Genetics & Heredity |
spellingShingle |
BMP signaling Dorsomorphin Dorsal-ventral patterning Shell field Mollusc Genetics & Heredity Tan, Sujian Huan, Pin Liu, Baozhong An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development |
topic_facet |
BMP signaling Dorsomorphin Dorsal-ventral patterning Shell field Mollusc Genetics & Heredity |
description |
Though the roles of BMP signaling in development is studied extensively in insects and vertebrates, our knowledge of BMP signaling in molluscan development is limited. In the present study, we performed a genome-based analysis of TGF-beta receptors in the Pacific oyster Crassostrea gigas and revealed that C. gigas possessed all five canonical members of the gene family, including three type I and two type II receptors. Whole mount in situ hybridization revealed that four receptor genes exhibited universal expression at the gastrula stage but cgi-bmprll mRNA was only expressed at the anterior lip of blastopore, indicating the regulation of BMP signaling through restricted expression of TGF-beta receptors. Treatment of oyster embryos using a BMP signaling inhibitor (dorsomorphin) resulted in obvious changes of the expression of developmental regulatory genes. In particular, three dorsally expressed genes were inhibited and two genes expressed at the opposite side showed increased expression, indicating BMP signaling may function in dorsal-ventral (DV) patterning. Some of the influenced genes were potential shell-formation (pSF) genes, including the well-accepted pSF gene engrailed, and thus this indicates the development of shell field was affected by dorsomorphin treatment. Subsequent scanning electronic observation revealed morphological change of the posterior margin of the shell field. Taken together, these results indicate the roles of BMP signaling in both DV patterning and development of shell field in oyster embryo, which expands our knowledge of early molluscan development. |
format |
Report |
author |
Tan, Sujian Huan, Pin Liu, Baozhong |
author_facet |
Tan, Sujian Huan, Pin Liu, Baozhong |
author_sort |
Tan, Sujian |
title |
An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development |
title_short |
An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development |
title_full |
An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development |
title_fullStr |
An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development |
title_full_unstemmed |
An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development |
title_sort |
investigation of oyster tgf-beta receptor genes and their potential roles in early molluscan development |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/159371 http://ir.qdio.ac.cn/handle/337002/159372 https://doi.org/10.1016/j.gene.2018.04.035 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
GENE http://ir.qdio.ac.cn/handle/337002/159371 http://ir.qdio.ac.cn/handle/337002/159372 doi:10.1016/j.gene.2018.04.035 |
op_doi |
https://doi.org/10.1016/j.gene.2018.04.035 |
container_title |
Gene |
container_volume |
663 |
container_start_page |
65 |
op_container_end_page |
71 |
_version_ |
1766394676542177280 |