Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas
As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of th...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Report |
Language: | English |
Published: |
FRONTIERS MEDIA SA
2018
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/159202 http://ir.qdio.ac.cn/handle/337002/159203 https://doi.org/10.3389/fimmu.2018.01280 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/159203 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/159203 2023-05-15T15:57:51+02:00 Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas Jiang, Shuai Qiu, Limei Wang, Lingling Jia, Zhihao Lv, Zhao Wang, Mengqiang Liu, Conghui Xu, Jiachao Song, Linsheng 2018-06-11 http://ir.qdio.ac.cn/handle/337002/159202 http://ir.qdio.ac.cn/handle/337002/159203 https://doi.org/10.3389/fimmu.2018.01280 英语 eng FRONTIERS MEDIA SA FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/159202 http://ir.qdio.ac.cn/handle/337002/159203 doi:10.3389/fimmu.2018.01280 Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L Immunology 期刊论文 2018 ftchinacasciocas https://doi.org/10.3389/fimmu.2018.01280 2022-06-27T05:40:01Z As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of this type from the Pacific oyster Crassostrea gigas were classified efficiently in this study via fluorescence-activated cell sorting (FACS) based on their phagocytosis of FITC-labeled latex beads. Transcriptomic and quantitative proteomic analyses revealed a series of differentially expressed genes (DEGs) and proteins present in phagocytes; of the 352 significantly high expressed proteins identified here within the phagocyte proteome, 262 corresponding genes were similarly high expressed in the transcriptome, while 140 of 205 significantly low expressed proteins within the proteome were transcriptionally low expressed. A pathway crosstalk network analysis of these significantly high expressed proteins revealed that phagocytes were highly activated in a number of antimicrobial-related biological processes, including oxidation-reduction and lysosomal proteolysis processes. A number of DEGs, including oxidase, lysosomal protease, and immune receptors, were also validated in this study using quantitative PCR, while seven lysosomal cysteine proteases, referred to as cathepsin Ls, were significantly high expressed in phagocytes. Results show that the expression level of cathepsin L protein in phagocytes [mean fluorescence intensity (MFI): 327 +/- 51] was significantly higher (p < 0.01) than that in non-phagocytic hemocytes (MFI: 83 +/- 26), while the cathepsin L protein was colocalized with the phagocytosed Vibrio splendidus in oyster hemocytes during this process. The results of this study collectively suggest that oyster phagocytes possess both potent oxidative killing and microbial disintegration capacities; these findings provide important insights into hemocyte phagocytic killing as a component of C. gigas innate ... Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Frontiers in Immunology 9 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L Immunology |
spellingShingle |
Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L Immunology Jiang, Shuai Qiu, Limei Wang, Lingling Jia, Zhihao Lv, Zhao Wang, Mengqiang Liu, Conghui Xu, Jiachao Song, Linsheng Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L Immunology |
description |
As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of this type from the Pacific oyster Crassostrea gigas were classified efficiently in this study via fluorescence-activated cell sorting (FACS) based on their phagocytosis of FITC-labeled latex beads. Transcriptomic and quantitative proteomic analyses revealed a series of differentially expressed genes (DEGs) and proteins present in phagocytes; of the 352 significantly high expressed proteins identified here within the phagocyte proteome, 262 corresponding genes were similarly high expressed in the transcriptome, while 140 of 205 significantly low expressed proteins within the proteome were transcriptionally low expressed. A pathway crosstalk network analysis of these significantly high expressed proteins revealed that phagocytes were highly activated in a number of antimicrobial-related biological processes, including oxidation-reduction and lysosomal proteolysis processes. A number of DEGs, including oxidase, lysosomal protease, and immune receptors, were also validated in this study using quantitative PCR, while seven lysosomal cysteine proteases, referred to as cathepsin Ls, were significantly high expressed in phagocytes. Results show that the expression level of cathepsin L protein in phagocytes [mean fluorescence intensity (MFI): 327 +/- 51] was significantly higher (p < 0.01) than that in non-phagocytic hemocytes (MFI: 83 +/- 26), while the cathepsin L protein was colocalized with the phagocytosed Vibrio splendidus in oyster hemocytes during this process. The results of this study collectively suggest that oyster phagocytes possess both potent oxidative killing and microbial disintegration capacities; these findings provide important insights into hemocyte phagocytic killing as a component of C. gigas innate ... |
format |
Report |
author |
Jiang, Shuai Qiu, Limei Wang, Lingling Jia, Zhihao Lv, Zhao Wang, Mengqiang Liu, Conghui Xu, Jiachao Song, Linsheng |
author_facet |
Jiang, Shuai Qiu, Limei Wang, Lingling Jia, Zhihao Lv, Zhao Wang, Mengqiang Liu, Conghui Xu, Jiachao Song, Linsheng |
author_sort |
Jiang, Shuai |
title |
Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas |
title_short |
Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas |
title_full |
Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas |
title_fullStr |
Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas |
title_full_unstemmed |
Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas |
title_sort |
transcriptomic and quantitative proteomic analyses provide insights into the phagocytic killing of hemocytes in the oyster crassostrea gigas |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/159202 http://ir.qdio.ac.cn/handle/337002/159203 https://doi.org/10.3389/fimmu.2018.01280 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/159202 http://ir.qdio.ac.cn/handle/337002/159203 doi:10.3389/fimmu.2018.01280 |
op_doi |
https://doi.org/10.3389/fimmu.2018.01280 |
container_title |
Frontiers in Immunology |
container_volume |
9 |
_version_ |
1766393554975850496 |