Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205)
Turbot (Schophthalmus maximus) is one of the most important economic marine flatfish species. However, due to rapid development of the industry, genetic resource recession has brought down the efficiency of aquaculture. Therefore, conservation of the genetic resource is increasingly demanded. Recent...
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Language: | English |
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ELSEVIER SCIENCE INC
2018
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Online Access: | http://ir.qdio.ac.cn/handle/337002/158966 http://ir.qdio.ac.cn/handle/337002/158967 https://doi.org/10.1016/j.theriogenology.2017.12.016 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/158967 2023-05-15T18:15:53+02:00 Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205) Zhao, Chunyan Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Wenqi Yang, Jingkun Yang, Yang Wang, Yanfeng Song, Zongcheng Li, Jun 2018-06-01 http://ir.qdio.ac.cn/handle/337002/158966 http://ir.qdio.ac.cn/handle/337002/158967 https://doi.org/10.1016/j.theriogenology.2017.12.016 英语 eng ELSEVIER SCIENCE INC THERIOGENOLOGY http://ir.qdio.ac.cn/handle/337002/158966 http://ir.qdio.ac.cn/handle/337002/158967 doi:10.1016/j.theriogenology.2017.12.016 Turbot Lymphocyte antigen 75 (ly75) Testis Spermatogonia Gonadal development Reproductive Biology Veterinary Sciences 期刊论文 2018 ftchinacasciocas https://doi.org/10.1016/j.theriogenology.2017.12.016 2022-06-27T05:39:55Z Turbot (Schophthalmus maximus) is one of the most important economic marine flatfish species. However, due to rapid development of the industry, genetic resource recession has brought down the efficiency of aquaculture. Therefore, conservation of the genetic resource is increasingly demanded. Recent research proved that type A spermatogonia possesses the properties of spermatogonia stem cell, and it might provide an ideal solution. Therefore, it is necessary to develop an appropriate molecular marker on type A spermatogonia to further isolate and purify of type A spermatogonia in turbot. In this study, turbot lymphocyte antigen 75 (smly75) gene was identified and its localizations of expressions and the temporal transcription patterns were evaluated qualitatively and semiquantitatively. Investigation in testes of development of spermatogonia showed that smly75 mRNA, contrast with vasa and dnd mRNA, was exclusively localized in type A spermatogonia and not detected in type B spermatogonia, spermatocytes or gonadal somatic cells by in situ hybridization. Thus, the smly75 could be a new and convincing molecular marker on identification of type A spermatogonia. In addition, specifically to development pattern of type A spermatogonia, from 7- to 14- month testes, spermatogonia were dominated and the number of type A spermatogonia was increased, corresponding that smly75 expression was up-regulated gradually, while, in 16 month testes, accompanied by that several spermatogonia differentiated into primary spermatocytes, the smly75 expression down-regulated. Finally, broaden in the whole reproductive cycle, the smly75 transcription significantly variated with the differentiation of germ cells and in accordance with the number of type A spermatogonia. It is suggested that testes from 8 to 14 month old males could be used for further isolation and purification of type A-SG. These results will not only help to better understand type A spermatogonia, but also further facilitate type A spematogonia-mediated germ cell ... Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Vasa ENVELOPE(25.177,25.177,67.587,67.587) Theriogenology 113 137 145 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Turbot Lymphocyte antigen 75 (ly75) Testis Spermatogonia Gonadal development Reproductive Biology Veterinary Sciences |
spellingShingle |
Turbot Lymphocyte antigen 75 (ly75) Testis Spermatogonia Gonadal development Reproductive Biology Veterinary Sciences Zhao, Chunyan Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Wenqi Yang, Jingkun Yang, Yang Wang, Yanfeng Song, Zongcheng Li, Jun Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205) |
topic_facet |
Turbot Lymphocyte antigen 75 (ly75) Testis Spermatogonia Gonadal development Reproductive Biology Veterinary Sciences |
description |
Turbot (Schophthalmus maximus) is one of the most important economic marine flatfish species. However, due to rapid development of the industry, genetic resource recession has brought down the efficiency of aquaculture. Therefore, conservation of the genetic resource is increasingly demanded. Recent research proved that type A spermatogonia possesses the properties of spermatogonia stem cell, and it might provide an ideal solution. Therefore, it is necessary to develop an appropriate molecular marker on type A spermatogonia to further isolate and purify of type A spermatogonia in turbot. In this study, turbot lymphocyte antigen 75 (smly75) gene was identified and its localizations of expressions and the temporal transcription patterns were evaluated qualitatively and semiquantitatively. Investigation in testes of development of spermatogonia showed that smly75 mRNA, contrast with vasa and dnd mRNA, was exclusively localized in type A spermatogonia and not detected in type B spermatogonia, spermatocytes or gonadal somatic cells by in situ hybridization. Thus, the smly75 could be a new and convincing molecular marker on identification of type A spermatogonia. In addition, specifically to development pattern of type A spermatogonia, from 7- to 14- month testes, spermatogonia were dominated and the number of type A spermatogonia was increased, corresponding that smly75 expression was up-regulated gradually, while, in 16 month testes, accompanied by that several spermatogonia differentiated into primary spermatocytes, the smly75 expression down-regulated. Finally, broaden in the whole reproductive cycle, the smly75 transcription significantly variated with the differentiation of germ cells and in accordance with the number of type A spermatogonia. It is suggested that testes from 8 to 14 month old males could be used for further isolation and purification of type A-SG. These results will not only help to better understand type A spermatogonia, but also further facilitate type A spematogonia-mediated germ cell ... |
format |
Report |
author |
Zhao, Chunyan Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Wenqi Yang, Jingkun Yang, Yang Wang, Yanfeng Song, Zongcheng Li, Jun |
author_facet |
Zhao, Chunyan Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Wenqi Yang, Jingkun Yang, Yang Wang, Yanfeng Song, Zongcheng Li, Jun |
author_sort |
Zhao, Chunyan |
title |
Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205) |
title_short |
Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205) |
title_full |
Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205) |
title_fullStr |
Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205) |
title_full_unstemmed |
Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205) |
title_sort |
identification of type a spermatogonia in turbot (scophthalmus maximus) using a new cell-surface marker of lymphocyte antigen 75 (ly75/cd205) |
publisher |
ELSEVIER SCIENCE INC |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/158966 http://ir.qdio.ac.cn/handle/337002/158967 https://doi.org/10.1016/j.theriogenology.2017.12.016 |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
THERIOGENOLOGY http://ir.qdio.ac.cn/handle/337002/158966 http://ir.qdio.ac.cn/handle/337002/158967 doi:10.1016/j.theriogenology.2017.12.016 |
op_doi |
https://doi.org/10.1016/j.theriogenology.2017.12.016 |
container_title |
Theriogenology |
container_volume |
113 |
container_start_page |
137 |
op_container_end_page |
145 |
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1766189141512421376 |