Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system
The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon (Salmo salar L.), the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system (RAS) to understand differences in the intestinal microbiota. The V4-V5 regions of the p...
Published in: | Journal of Oceanology and Limnology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/158918 2023-05-15T15:30:47+02:00 Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system Wang Chun Sun Guoxiang Li Shuangshuang Li Xian Liu Ying 2018-03-01 http://ir.qdio.ac.cn/handle/337002/158918 https://doi.org/10.1007/s00343-017-6203-5 英语 eng SCIENCE PRESS JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.qdio.ac.cn/handle/337002/158918 doi:10.1007/s00343-017-6203-5 intestinal microbiota health status Atlantic salmon (Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing Marine & Freshwater Biology Oceanography Limnology TROUT ONCORHYNCHUS-MYKISS EARLY-LIFE STAGES GUT MICROBIOTA AEROMONAS-SALMONICIDA BACTERIAL COMMUNITIES GRASS CARP CTENOPHARYNGODON-IDELLUS MOLECULAR ANALYSIS FISH LARVAE DIVERSITY 期刊论文 2018 ftchinacasciocas https://doi.org/10.1007/s00343-017-6203-5 2022-06-27T05:39:55Z The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon (Salmo salar L.), the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system (RAS) to understand differences in the intestinal microbiota. The V4-V5 regions of the prokaryotic 16S rRNA genes in the samples were analyzed by MiSeq high-throughput sequencing. The fish were adults with no differences in body length or weight. Representative members of the intestinal microbiota were identified. The intestinal microbiota of the healthy fish included Proteobacteria (44.33%), Actinobacteria (17.89%), Bacteroidetes (15.25%), and Firmicutes (9.11%), among which the families Micrococcaceae and Oxalobacteraceae and genera Sphingomonas, Streptomyces, Pedobacter, Janthinobacterium, Burkholderia, and Balneimonas were most abundant. Proteobacteria (70.46%), Bacteroidetes (7.59%), and Firmicutes (7.55%) dominated the microbiota of unhealthy fish, and Chloroflexi (2.71%), and Aliivibrio and Vibrio as well as genera in the family Aeromonadaceae were most strongly represented. Overall, the intestinal hindgut microbiota differed between healthy and unhealthy fish. This study offers a useful tool for monitoring the health status of fish and for screening the utility of probiotics by studying the intestinal microbiota. Report Atlantic salmon Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Journal of Oceanology and Limnology 36 2 414 426 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
intestinal microbiota health status Atlantic salmon (Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing Marine & Freshwater Biology Oceanography Limnology TROUT ONCORHYNCHUS-MYKISS EARLY-LIFE STAGES GUT MICROBIOTA AEROMONAS-SALMONICIDA BACTERIAL COMMUNITIES GRASS CARP CTENOPHARYNGODON-IDELLUS MOLECULAR ANALYSIS FISH LARVAE DIVERSITY |
spellingShingle |
intestinal microbiota health status Atlantic salmon (Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing Marine & Freshwater Biology Oceanography Limnology TROUT ONCORHYNCHUS-MYKISS EARLY-LIFE STAGES GUT MICROBIOTA AEROMONAS-SALMONICIDA BACTERIAL COMMUNITIES GRASS CARP CTENOPHARYNGODON-IDELLUS MOLECULAR ANALYSIS FISH LARVAE DIVERSITY Wang Chun Sun Guoxiang Li Shuangshuang Li Xian Liu Ying Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system |
topic_facet |
intestinal microbiota health status Atlantic salmon (Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing Marine & Freshwater Biology Oceanography Limnology TROUT ONCORHYNCHUS-MYKISS EARLY-LIFE STAGES GUT MICROBIOTA AEROMONAS-SALMONICIDA BACTERIAL COMMUNITIES GRASS CARP CTENOPHARYNGODON-IDELLUS MOLECULAR ANALYSIS FISH LARVAE DIVERSITY |
description |
The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon (Salmo salar L.), the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system (RAS) to understand differences in the intestinal microbiota. The V4-V5 regions of the prokaryotic 16S rRNA genes in the samples were analyzed by MiSeq high-throughput sequencing. The fish were adults with no differences in body length or weight. Representative members of the intestinal microbiota were identified. The intestinal microbiota of the healthy fish included Proteobacteria (44.33%), Actinobacteria (17.89%), Bacteroidetes (15.25%), and Firmicutes (9.11%), among which the families Micrococcaceae and Oxalobacteraceae and genera Sphingomonas, Streptomyces, Pedobacter, Janthinobacterium, Burkholderia, and Balneimonas were most abundant. Proteobacteria (70.46%), Bacteroidetes (7.59%), and Firmicutes (7.55%) dominated the microbiota of unhealthy fish, and Chloroflexi (2.71%), and Aliivibrio and Vibrio as well as genera in the family Aeromonadaceae were most strongly represented. Overall, the intestinal hindgut microbiota differed between healthy and unhealthy fish. This study offers a useful tool for monitoring the health status of fish and for screening the utility of probiotics by studying the intestinal microbiota. |
format |
Report |
author |
Wang Chun Sun Guoxiang Li Shuangshuang Li Xian Liu Ying |
author_facet |
Wang Chun Sun Guoxiang Li Shuangshuang Li Xian Liu Ying |
author_sort |
Wang Chun |
title |
Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system |
title_short |
Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system |
title_full |
Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system |
title_fullStr |
Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system |
title_full_unstemmed |
Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system |
title_sort |
intestinal microbiota of healthy and unhealthy atlantic salmon salmo salar l. in a recirculating aquaculture system |
publisher |
SCIENCE PRESS |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/158918 https://doi.org/10.1007/s00343-017-6203-5 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.qdio.ac.cn/handle/337002/158918 doi:10.1007/s00343-017-6203-5 |
op_doi |
https://doi.org/10.1007/s00343-017-6203-5 |
container_title |
Journal of Oceanology and Limnology |
container_volume |
36 |
container_issue |
2 |
container_start_page |
414 |
op_container_end_page |
426 |
_version_ |
1766361235767427072 |