Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis
Increasing evidence has demonstrated support for the endocytic capacities of teleost B cells. In the present study, the ability of turbot IgM(+) B cells to ingest microspheres of different sizes and the corresponding internalization pathways were investigated. The results showed that IgM(+) B cells...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/158340 https://doi.org/10.1016/j.dci.2017.12.028 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/158340 2023-05-15T18:15:44+02:00 Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis Li, Yi-qun Sun, Li Li, Jun 2018-05-01 http://ir.qdio.ac.cn/handle/337002/158340 https://doi.org/10.1016/j.dci.2017.12.028 英语 eng ELSEVIER SCI LTD DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/158340 doi:10.1016/j.dci.2017.12.028 Scophthalmus maximus IgM(+) cells Endocytosis Macropinocytosis Fisheries Immunology Zoology EDWARDSIELLA-TARDA TELEOST FISH T-CELLS LYMPHOCYTES ANTIGEN IDENTIFICATION MECHANISMS PHAGOCYTES IMMUNITY 期刊论文 2018 ftchinacasciocas https://doi.org/10.1016/j.dci.2017.12.028 2022-06-27T05:39:43Z Increasing evidence has demonstrated support for the endocytic capacities of teleost B cells. In the present study, the ability of turbot IgM(+) B cells to ingest microspheres of different sizes and the corresponding internalization pathways were investigated. The results showed that IgM(+) B cells exhibited relatively high endocytic capacities for 0.5 mu m and 1 mu m latex beads, and that different mechanisms were employed for IgM(+) and IgM(-) cells to uptake 0.5 gm and 1 gm beads. For 0.5 gm beads, IgM(+) B cells apparently employed macropinocytosis-dependent endocytic pathway, whereas IgM(-) cells utilized a different process involving both clathrin-and caveolae-mediated pathways. For the uptake of 1 mu m beads, IgM(+) cells relied mainly on macropinocytosis and partially on caveolae-mediated pathway, while IgM(-) cells utilized the routes similar to that of internalizing 0.5 mu m beads. Consistently, the internalized microspheres were co-localized with high-molecular-mass dextran in IgM(+) phagocytic cells. In addition to latex beads, IgM(+) B cells could also ingest inactivated bacteria predominately through macropinocytosis and caveolae-mediated endocytosis. These results collectively indicated that macropinocytosis is principally responsible for particle uptake by turbot IgM(+) B cells. (C) 2018 Elsevier Ltd. All rights reserved. Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 82 31 38 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Scophthalmus maximus IgM(+) cells Endocytosis Macropinocytosis Fisheries Immunology Zoology EDWARDSIELLA-TARDA TELEOST FISH T-CELLS LYMPHOCYTES ANTIGEN IDENTIFICATION MECHANISMS PHAGOCYTES IMMUNITY |
spellingShingle |
Scophthalmus maximus IgM(+) cells Endocytosis Macropinocytosis Fisheries Immunology Zoology EDWARDSIELLA-TARDA TELEOST FISH T-CELLS LYMPHOCYTES ANTIGEN IDENTIFICATION MECHANISMS PHAGOCYTES IMMUNITY Li, Yi-qun Sun, Li Li, Jun Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis |
topic_facet |
Scophthalmus maximus IgM(+) cells Endocytosis Macropinocytosis Fisheries Immunology Zoology EDWARDSIELLA-TARDA TELEOST FISH T-CELLS LYMPHOCYTES ANTIGEN IDENTIFICATION MECHANISMS PHAGOCYTES IMMUNITY |
description |
Increasing evidence has demonstrated support for the endocytic capacities of teleost B cells. In the present study, the ability of turbot IgM(+) B cells to ingest microspheres of different sizes and the corresponding internalization pathways were investigated. The results showed that IgM(+) B cells exhibited relatively high endocytic capacities for 0.5 mu m and 1 mu m latex beads, and that different mechanisms were employed for IgM(+) and IgM(-) cells to uptake 0.5 gm and 1 gm beads. For 0.5 gm beads, IgM(+) B cells apparently employed macropinocytosis-dependent endocytic pathway, whereas IgM(-) cells utilized a different process involving both clathrin-and caveolae-mediated pathways. For the uptake of 1 mu m beads, IgM(+) cells relied mainly on macropinocytosis and partially on caveolae-mediated pathway, while IgM(-) cells utilized the routes similar to that of internalizing 0.5 mu m beads. Consistently, the internalized microspheres were co-localized with high-molecular-mass dextran in IgM(+) phagocytic cells. In addition to latex beads, IgM(+) B cells could also ingest inactivated bacteria predominately through macropinocytosis and caveolae-mediated endocytosis. These results collectively indicated that macropinocytosis is principally responsible for particle uptake by turbot IgM(+) B cells. (C) 2018 Elsevier Ltd. All rights reserved. |
format |
Report |
author |
Li, Yi-qun Sun, Li Li, Jun |
author_facet |
Li, Yi-qun Sun, Li Li, Jun |
author_sort |
Li, Yi-qun |
title |
Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis |
title_short |
Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis |
title_full |
Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis |
title_fullStr |
Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis |
title_full_unstemmed |
Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis |
title_sort |
internalization of large particles by turbot (scophthalmus maximus) igm(+) b cells mainly depends on macropinocytosis |
publisher |
ELSEVIER SCI LTD |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/158340 https://doi.org/10.1016/j.dci.2017.12.028 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/158340 doi:10.1016/j.dci.2017.12.028 |
op_doi |
https://doi.org/10.1016/j.dci.2017.12.028 |
container_title |
Developmental & Comparative Immunology |
container_volume |
82 |
container_start_page |
31 |
op_container_end_page |
38 |
_version_ |
1766188931173318656 |