CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas
Toll like receptor (TLR) signaling cascades are under precise regulations to ensure the proper immune responses during various pathogen invasions. The neuregulin receptor degradation protein-1 (Nrdp1) has been demonstrated to be a novel negative regulator of TLR signaling by targeting MyD88 to induc...
Published in: | Fish & Shellfish Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/158121 https://doi.org/10.1016/j.fsi.2017.12.057 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/158121 2023-05-15T15:57:44+02:00 CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas Sun, Rui Qiu, Limei Yi, Qilin Wang, Mengqiang Yue, Feng Wang, Lingling Song, Linsheng 2018-03-01 http://ir.qdio.ac.cn/handle/337002/158121 https://doi.org/10.1016/j.fsi.2017.12.057 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/158121 doi:10.1016/j.fsi.2017.12.057 Nrdp1 Toll like receptor signaling pathway Immune regulation Evolution of innate immunity Crassostrea gigas Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences RESPONSES MYD88 UBIQUITINATION DEGRADATION PATHWAYS GROWTH GENE FORM 期刊论文 2018 ftchinacasciocas https://doi.org/10.1016/j.fsi.2017.12.057 2022-06-27T05:39:38Z Toll like receptor (TLR) signaling cascades are under precise regulations to ensure the proper immune responses during various pathogen invasions. The neuregulin receptor degradation protein-1 (Nrdp1) has been demonstrated to be a novel negative regulator of TLR signaling by targeting MyD88 to induce degradation in mammals. In the present study, an Nrdp1 homologue, CgNrdp1, was identified from the genome of Pacific oyster Crassostrea gigas. It contained an open reading frame encoding a polypeptide of 315 amino acids which shared high identities with other homologues from different species. There was a conserved RING domain in CgNrdp1, indicating the functional E3 ubiquitin ligase activity. The bacterially expressed recombinant CgNrdp1 and CgMyD88 showed much stronger affinity compared to control groups in the ELISA assay, showing the interacting ability between CgNrdp1 and CgMyD88. When CgMyD88 or HsMyD88 was co-transfected with CgNrdp1 into HEK293T cells, the luciferase activities of NF-kappa B were significantly decreased compared to those in MyD88 single-transfection groups, indicating the conserved negative regulating function of CgNrdp1 on the MyD88 induced TLR signaling. These results indicated that CgNrdp1 was a negative regulator of TLR signaling in oyster and the Nrdp1-MyD88 axis was functional and highly conserved from mollusks to mammals in the negative regulation of TLR signaling. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Fish & Shellfish Immunology 74 386 392 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Nrdp1 Toll like receptor signaling pathway Immune regulation Evolution of innate immunity Crassostrea gigas Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences RESPONSES MYD88 UBIQUITINATION DEGRADATION PATHWAYS GROWTH GENE FORM |
spellingShingle |
Nrdp1 Toll like receptor signaling pathway Immune regulation Evolution of innate immunity Crassostrea gigas Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences RESPONSES MYD88 UBIQUITINATION DEGRADATION PATHWAYS GROWTH GENE FORM Sun, Rui Qiu, Limei Yi, Qilin Wang, Mengqiang Yue, Feng Wang, Lingling Song, Linsheng CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas |
topic_facet |
Nrdp1 Toll like receptor signaling pathway Immune regulation Evolution of innate immunity Crassostrea gigas Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences RESPONSES MYD88 UBIQUITINATION DEGRADATION PATHWAYS GROWTH GENE FORM |
description |
Toll like receptor (TLR) signaling cascades are under precise regulations to ensure the proper immune responses during various pathogen invasions. The neuregulin receptor degradation protein-1 (Nrdp1) has been demonstrated to be a novel negative regulator of TLR signaling by targeting MyD88 to induce degradation in mammals. In the present study, an Nrdp1 homologue, CgNrdp1, was identified from the genome of Pacific oyster Crassostrea gigas. It contained an open reading frame encoding a polypeptide of 315 amino acids which shared high identities with other homologues from different species. There was a conserved RING domain in CgNrdp1, indicating the functional E3 ubiquitin ligase activity. The bacterially expressed recombinant CgNrdp1 and CgMyD88 showed much stronger affinity compared to control groups in the ELISA assay, showing the interacting ability between CgNrdp1 and CgMyD88. When CgMyD88 or HsMyD88 was co-transfected with CgNrdp1 into HEK293T cells, the luciferase activities of NF-kappa B were significantly decreased compared to those in MyD88 single-transfection groups, indicating the conserved negative regulating function of CgNrdp1 on the MyD88 induced TLR signaling. These results indicated that CgNrdp1 was a negative regulator of TLR signaling in oyster and the Nrdp1-MyD88 axis was functional and highly conserved from mollusks to mammals in the negative regulation of TLR signaling. |
format |
Report |
author |
Sun, Rui Qiu, Limei Yi, Qilin Wang, Mengqiang Yue, Feng Wang, Lingling Song, Linsheng |
author_facet |
Sun, Rui Qiu, Limei Yi, Qilin Wang, Mengqiang Yue, Feng Wang, Lingling Song, Linsheng |
author_sort |
Sun, Rui |
title |
CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas |
title_short |
CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas |
title_full |
CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas |
title_fullStr |
CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas |
title_full_unstemmed |
CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas |
title_sort |
cgnrdp1, a conserved negative regulating factor of myd88-dependent toll like receptor signaling in oyster crassostrea gigas |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/158121 https://doi.org/10.1016/j.fsi.2017.12.057 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/158121 doi:10.1016/j.fsi.2017.12.057 |
op_doi |
https://doi.org/10.1016/j.fsi.2017.12.057 |
container_title |
Fish & Shellfish Immunology |
container_volume |
74 |
container_start_page |
386 |
op_container_end_page |
392 |
_version_ |
1766393433636732928 |