Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation
As marine invertebrates, oysters lack adaptive immunity and employ innate immunity as the front line and almost the solo defense mechanism to protect them against invaders. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to host defe...
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Online Access: | http://ir.qdio.ac.cn/handle/337002/158118 https://doi.org/10.1016/j.fsi.2018.01.017 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/158118 2023-05-15T15:57:51+02:00 Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation Wang, Mengqiang Liu, Mei Wang, Baojie Jiang, Keyong Jia, Zhihao Wang, Lingling Wang, Lei 2018-03-01 http://ir.qdio.ac.cn/handle/337002/158118 https://doi.org/10.1016/j.fsi.2018.01.017 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/158118 doi:10.1016/j.fsi.2018.01.017 Crassostrea gigas Exosomes Extracellular vesicles Innate immunity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences FALSE DISCOVERY RATE GROUP BOX 1 EXPRESSION ANALYSIS IMMUNE PROTEIN APOPTOSIS DISEASE GENES INVERTEBRATES ADAPTATION 期刊论文 2018 ftchinacasciocas https://doi.org/10.1016/j.fsi.2018.01.017 2022-06-27T05:39:38Z As marine invertebrates, oysters lack adaptive immunity and employ innate immunity as the front line and almost the solo defense mechanism to protect them against invaders. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to host defense mechanism. To better understand the immune functions of exosomes in Crassostrea gigas against bacterial stimulation, RNA-Seq was applied to explore the global expression changes of exosomes in oyster after Staphylococcus aureus and Vibrio splendidus stimulation. Totally 171573691 single end raw reads were yielded via Ion Torrent Proton sequencing, which were trimmed into 121988325 clean reads, and then 1505 abundant exosomal shuttle mRNAs (esmRNAs) were identified. Gene ontology (GO) analysis revealed that these abundant esmRNAs could be categorized into 15 cellular components, 12 molecular functions and 21 biological processes, and these abundant esmRNAs were mapped onto 62 biological signaling pathways by KEGG. In total, 68 significant differentially expressed genes (DEGs, Fold change >= 2, Q-value < 0.05) were identified between S. aureus stimulated group and control group, including 21 up-regulated and 47 down regulated ones. While 99 significant DEGs between V. splendidus challenged group and control group were identified, including 42 up-regulated and 57 down-regulated ones. To validate the transcriptomic data, 24 DEGs were randomly selected and confirmed via quantitative real-time PCR (qRT-PCR) and the results showed that their expression patterns agreed well with the RNA-Seq analysis. This study would enrich the C. gigas transcriptome database and provide insight into the immune functions of oyster exosomes against bacterial infection. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Fish & Shellfish Immunology 74 540 550 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea gigas Exosomes Extracellular vesicles Innate immunity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences FALSE DISCOVERY RATE GROUP BOX 1 EXPRESSION ANALYSIS IMMUNE PROTEIN APOPTOSIS DISEASE GENES INVERTEBRATES ADAPTATION |
spellingShingle |
Crassostrea gigas Exosomes Extracellular vesicles Innate immunity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences FALSE DISCOVERY RATE GROUP BOX 1 EXPRESSION ANALYSIS IMMUNE PROTEIN APOPTOSIS DISEASE GENES INVERTEBRATES ADAPTATION Wang, Mengqiang Liu, Mei Wang, Baojie Jiang, Keyong Jia, Zhihao Wang, Lingling Wang, Lei Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation |
topic_facet |
Crassostrea gigas Exosomes Extracellular vesicles Innate immunity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences FALSE DISCOVERY RATE GROUP BOX 1 EXPRESSION ANALYSIS IMMUNE PROTEIN APOPTOSIS DISEASE GENES INVERTEBRATES ADAPTATION |
description |
As marine invertebrates, oysters lack adaptive immunity and employ innate immunity as the front line and almost the solo defense mechanism to protect them against invaders. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to host defense mechanism. To better understand the immune functions of exosomes in Crassostrea gigas against bacterial stimulation, RNA-Seq was applied to explore the global expression changes of exosomes in oyster after Staphylococcus aureus and Vibrio splendidus stimulation. Totally 171573691 single end raw reads were yielded via Ion Torrent Proton sequencing, which were trimmed into 121988325 clean reads, and then 1505 abundant exosomal shuttle mRNAs (esmRNAs) were identified. Gene ontology (GO) analysis revealed that these abundant esmRNAs could be categorized into 15 cellular components, 12 molecular functions and 21 biological processes, and these abundant esmRNAs were mapped onto 62 biological signaling pathways by KEGG. In total, 68 significant differentially expressed genes (DEGs, Fold change >= 2, Q-value < 0.05) were identified between S. aureus stimulated group and control group, including 21 up-regulated and 47 down regulated ones. While 99 significant DEGs between V. splendidus challenged group and control group were identified, including 42 up-regulated and 57 down-regulated ones. To validate the transcriptomic data, 24 DEGs were randomly selected and confirmed via quantitative real-time PCR (qRT-PCR) and the results showed that their expression patterns agreed well with the RNA-Seq analysis. This study would enrich the C. gigas transcriptome database and provide insight into the immune functions of oyster exosomes against bacterial infection. |
format |
Report |
author |
Wang, Mengqiang Liu, Mei Wang, Baojie Jiang, Keyong Jia, Zhihao Wang, Lingling Wang, Lei |
author_facet |
Wang, Mengqiang Liu, Mei Wang, Baojie Jiang, Keyong Jia, Zhihao Wang, Lingling Wang, Lei |
author_sort |
Wang, Mengqiang |
title |
Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation |
title_short |
Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation |
title_full |
Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation |
title_fullStr |
Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation |
title_full_unstemmed |
Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation |
title_sort |
transcriptomic analysis of exosomal shuttle mrna in pacific oyster crassostrea gigas during bacterial stimulation |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/158118 https://doi.org/10.1016/j.fsi.2018.01.017 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/158118 doi:10.1016/j.fsi.2018.01.017 |
op_doi |
https://doi.org/10.1016/j.fsi.2018.01.017 |
container_title |
Fish & Shellfish Immunology |
container_volume |
74 |
container_start_page |
540 |
op_container_end_page |
550 |
_version_ |
1766393554063589376 |