Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus
A worldwide increase in the reports of diseases affecting marine organisms has paralleled the climate warming over the past few decades. In this study, we applied omics to explore the mechanisms underlying thermo-linked epizootics, by comparing both the transcriptome- and proteome-wide response of t...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/157997 2023-05-15T18:15:50+02:00 Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus Yue, Xin Huan, Pin Hu, Yonghua Liu, Baozhong 2018-01-30 http://ir.qdio.ac.cn/handle/337002/157997 https://doi.org/10.1038/s41598-018-20065-1 英语 eng NATURE PUBLISHING GROUP SCIENTIFIC REPORTS http://ir.qdio.ac.cn/handle/337002/157997 doi:10.1038/s41598-018-20065-1 Science & Technology - Other Topics Multidisciplinary Sciences DOUBLE-STRANDED-RNA POLY-I-C RAINBOW-TROUT OXIDATIVE STRESS CLIMATE-CHANGE ENVIRONMENTAL-TEMPERATURE EXPERIMENTAL-INFECTION ENDOPLASMIC-RETICULUM MOLECULAR-MECHANISMS ONCORHYNCHUS-MYKISS 期刊论文 2018 ftchinacasciocas https://doi.org/10.1038/s41598-018-20065-1 2022-06-27T05:39:38Z A worldwide increase in the reports of diseases affecting marine organisms has paralleled the climate warming over the past few decades. In this study, we applied omics to explore the mechanisms underlying thermo-linked epizootics, by comparing both the transcriptome- and proteome-wide response of turbots to a mimic pathogen (poly I:C) between high temperature and low temperature using a time-course approach. Our results showed that myeloperoxidase (MPO) and insulin were differentially expressed transcripts shared by all five time-points post poly I:C-injection between high and low temperature and also had a consistent expression trend as differentially expressed proteins at 24 h post injection. Combined with other data, it was suggested that the elevated temperature enhanced neutrophil-mediated immunity and the resultant MPO-mediated oxidative stress, which lasted for at least 5 days. The contents of malondialdehyde and protein carbonyls, markers of oxidative damage for lipids and proteins, respectively, were compared between different temperature groups, and the results further implied the emergence of oxidative damage under high temperature. It was also suggested that metabolism disorder likely occur considering the sustained expression changes of insulin. Hence, prolonged MPO-mediated oxidative stress and metabolic disorder might be involved in the thermo-linked epizootic. Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Scientific Reports 8 1 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Science & Technology - Other Topics Multidisciplinary Sciences DOUBLE-STRANDED-RNA POLY-I-C RAINBOW-TROUT OXIDATIVE STRESS CLIMATE-CHANGE ENVIRONMENTAL-TEMPERATURE EXPERIMENTAL-INFECTION ENDOPLASMIC-RETICULUM MOLECULAR-MECHANISMS ONCORHYNCHUS-MYKISS |
spellingShingle |
Science & Technology - Other Topics Multidisciplinary Sciences DOUBLE-STRANDED-RNA POLY-I-C RAINBOW-TROUT OXIDATIVE STRESS CLIMATE-CHANGE ENVIRONMENTAL-TEMPERATURE EXPERIMENTAL-INFECTION ENDOPLASMIC-RETICULUM MOLECULAR-MECHANISMS ONCORHYNCHUS-MYKISS Yue, Xin Huan, Pin Hu, Yonghua Liu, Baozhong Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus |
topic_facet |
Science & Technology - Other Topics Multidisciplinary Sciences DOUBLE-STRANDED-RNA POLY-I-C RAINBOW-TROUT OXIDATIVE STRESS CLIMATE-CHANGE ENVIRONMENTAL-TEMPERATURE EXPERIMENTAL-INFECTION ENDOPLASMIC-RETICULUM MOLECULAR-MECHANISMS ONCORHYNCHUS-MYKISS |
description |
A worldwide increase in the reports of diseases affecting marine organisms has paralleled the climate warming over the past few decades. In this study, we applied omics to explore the mechanisms underlying thermo-linked epizootics, by comparing both the transcriptome- and proteome-wide response of turbots to a mimic pathogen (poly I:C) between high temperature and low temperature using a time-course approach. Our results showed that myeloperoxidase (MPO) and insulin were differentially expressed transcripts shared by all five time-points post poly I:C-injection between high and low temperature and also had a consistent expression trend as differentially expressed proteins at 24 h post injection. Combined with other data, it was suggested that the elevated temperature enhanced neutrophil-mediated immunity and the resultant MPO-mediated oxidative stress, which lasted for at least 5 days. The contents of malondialdehyde and protein carbonyls, markers of oxidative damage for lipids and proteins, respectively, were compared between different temperature groups, and the results further implied the emergence of oxidative damage under high temperature. It was also suggested that metabolism disorder likely occur considering the sustained expression changes of insulin. Hence, prolonged MPO-mediated oxidative stress and metabolic disorder might be involved in the thermo-linked epizootic. |
format |
Report |
author |
Yue, Xin Huan, Pin Hu, Yonghua Liu, Baozhong |
author_facet |
Yue, Xin Huan, Pin Hu, Yonghua Liu, Baozhong |
author_sort |
Yue, Xin |
title |
Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus |
title_short |
Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus |
title_full |
Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus |
title_fullStr |
Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus |
title_full_unstemmed |
Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus |
title_sort |
integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot scophthalmus maximus |
publisher |
NATURE PUBLISHING GROUP |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/157997 https://doi.org/10.1038/s41598-018-20065-1 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
SCIENTIFIC REPORTS http://ir.qdio.ac.cn/handle/337002/157997 doi:10.1038/s41598-018-20065-1 |
op_doi |
https://doi.org/10.1038/s41598-018-20065-1 |
container_title |
Scientific Reports |
container_volume |
8 |
container_issue |
1 |
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1766189074066964480 |