ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection
Aldehyde dehydrogenases (ALDHs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. However, the activity and function of these detoxifying proteins remain unknown, especially...
Published in: | Fish & Shellfish Immunology |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/157315 2023-05-15T15:30:32+02:00 ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying 2017-11-01 http://ir.qdio.ac.cn/handle/337002/157314 http://ir.qdio.ac.cn/handle/337002/157315 https://doi.org/10.1016/j.fsi.2017.08.043 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/157314 http://ir.qdio.ac.cn/handle/337002/157315 doi:10.1016/j.fsi.2017.08.043 ALDH7A1 Aeromonas salmonicida Atlantic salmon Recombinant protein Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.fsi.2017.08.043 2022-06-27T05:39:24Z Aldehyde dehydrogenases (ALDHs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. However, the activity and function of these detoxifying proteins remain unknown, especially in fish. In our research, we aimed to study functions of aldehyde dehydrogenase 7A1 (ALDH7A1) in Atlantic salmon infected by Aeromonas salmonicida. Recombinant ALDH7A1 (rALDH7A1) was verified by SDS-PAGE and western blot. The molecular mass of the deduced amino acid sequence of rALDH7A1 is 58.9 kDa with an estimated pI of 7.09. Only a low complexity region ((141)yvegvgevqeyvdv(153)) without a signal peptide existed in rALDH7A1. Results of ELISA indicated that rALDH7A1 exhibited apparent binding activities with A. salmonicida and its expression was highest in fish kidney. A Real Time PCR (qRT-PCR) assay in kidneys confirmed that fish in this experiment were authentically infected and bacterial loads in rALDH7A1-adminsitered fish were significantly reduced at an early stage of infection. Meanwhile, we found the mRNA expression of NF-k beta, P-38 MAPK, caspase-3 and TNF-alpha were mainly up regulated at 72 h in the kidneys and livers of highly infected fish injected with rALDH7A1, and the same variation trend existed in fish spleens at 12 h. Consistent with these observations, neutralization experiments in vivo indicated that rALDH7A1 could obviously reduce the death rate compared to the BSA and control group. Taken together, we concluded that rALDH7A1 could act in host immune defense against bacterial infection and decrease the mortality rate of Atlantic salmon at early stages of infection with A. salmonicida. (C) 2017 Elsevier Ltd. All rights reserved. Report Atlantic salmon Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 70 30 39 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
ALDH7A1 Aeromonas salmonicida Atlantic salmon Recombinant protein Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
ALDH7A1 Aeromonas salmonicida Atlantic salmon Recombinant protein Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection |
topic_facet |
ALDH7A1 Aeromonas salmonicida Atlantic salmon Recombinant protein Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Aldehyde dehydrogenases (ALDHs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. However, the activity and function of these detoxifying proteins remain unknown, especially in fish. In our research, we aimed to study functions of aldehyde dehydrogenase 7A1 (ALDH7A1) in Atlantic salmon infected by Aeromonas salmonicida. Recombinant ALDH7A1 (rALDH7A1) was verified by SDS-PAGE and western blot. The molecular mass of the deduced amino acid sequence of rALDH7A1 is 58.9 kDa with an estimated pI of 7.09. Only a low complexity region ((141)yvegvgevqeyvdv(153)) without a signal peptide existed in rALDH7A1. Results of ELISA indicated that rALDH7A1 exhibited apparent binding activities with A. salmonicida and its expression was highest in fish kidney. A Real Time PCR (qRT-PCR) assay in kidneys confirmed that fish in this experiment were authentically infected and bacterial loads in rALDH7A1-adminsitered fish were significantly reduced at an early stage of infection. Meanwhile, we found the mRNA expression of NF-k beta, P-38 MAPK, caspase-3 and TNF-alpha were mainly up regulated at 72 h in the kidneys and livers of highly infected fish injected with rALDH7A1, and the same variation trend existed in fish spleens at 12 h. Consistent with these observations, neutralization experiments in vivo indicated that rALDH7A1 could obviously reduce the death rate compared to the BSA and control group. Taken together, we concluded that rALDH7A1 could act in host immune defense against bacterial infection and decrease the mortality rate of Atlantic salmon at early stages of infection with A. salmonicida. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Report |
author |
Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying |
author_facet |
Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying |
author_sort |
Liu, Peng-fei |
title |
ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection |
title_short |
ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection |
title_full |
ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection |
title_fullStr |
ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection |
title_full_unstemmed |
ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection |
title_sort |
aldh7a1 is a protein that protects atlantic salmon against aeromonas salmonicida at the early stages of infection |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/157314 http://ir.qdio.ac.cn/handle/337002/157315 https://doi.org/10.1016/j.fsi.2017.08.043 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/157314 http://ir.qdio.ac.cn/handle/337002/157315 doi:10.1016/j.fsi.2017.08.043 |
op_doi |
https://doi.org/10.1016/j.fsi.2017.08.043 |
container_title |
Fish & Shellfish Immunology |
container_volume |
70 |
container_start_page |
30 |
op_container_end_page |
39 |
_version_ |
1766360982994550784 |