The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas
Pattern recognition receptors (PRRs) are essential in recognizing specific pathogen-associated molecular patterns (PAMPs) on microbes and triggering responses to eliminate the invading pathogens. Previous genomic studies have revealed a great number of PRR genes in the Pacific oyster Crassostrea gig...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/156145 http://ir.qdio.ac.cn/handle/337002/156146 https://doi.org/10.1016/j.dci.2018.09.021 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/156145 2023-05-15T15:58:03+02:00 The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas Wang, Lingling Zhang, Huan Wang, Mengqiang Zhou, Zhi Wang, Weilin Liu, Rui Huang, Mengmeng Yang, Chuanyan Qiu, Limei Song, Linsheng 2019-02-01 http://ir.qdio.ac.cn/handle/337002/156145 http://ir.qdio.ac.cn/handle/337002/156146 https://doi.org/10.1016/j.dci.2018.09.021 英语 eng ELSEVIER SCI LTD DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/156145 http://ir.qdio.ac.cn/handle/337002/156146 doi:10.1016/j.dci.2018.09.021 Bivalve immunity Pattern recognition receptors Crassostrea gigas Transcriptome RNA-Seq Fisheries Immunology Zoology 期刊论文 2019 ftchinacasciocas https://doi.org/10.1016/j.dci.2018.09.021 2022-07-29T12:11:37Z Pattern recognition receptors (PRRs) are essential in recognizing specific pathogen-associated molecular patterns (PAMPs) on microbes and triggering responses to eliminate the invading pathogens. Previous genomic studies have revealed a great number of PRR genes in the Pacific oyster Crassostrea gigas, a sessile and filter-feeder marine bivalve belonging to the phylum Mollusca. On the survey of PRRs in the assembly oyster reference genome version 9, a total of 1084 PRRs were identified, which were composed of at least 12 gene families. Some of the gene families were significantly expanded, including C-type lectins (CTLs), fibrinogen-related proteins (FREPs), scavenger receptor cysteine-rich repeat protein (SRCRs), leucine-rich repeat (LRR)-only proteins (LRRops), and especially Clq domain-containing proteins (ClqDCs). The transcriptomic profiles of these abundant PRRs in response to PAMP treatments were investigated by RNA-Seq using the SOLiD EZ BeadTM system. Compared to the control library, there were 6,655, 7,273, 7,593, 6,830, 6687 and 8250 differentially expressed genes in the haemocytes of oysters in response to lipopolysaccharide (LPS) stimulation for 6 h, 12 h and 24 h, and peptidoglycan (PGN), glucan (GLU) and poly I:C (IC) stimulation for 12 h, respectively. After stimulation for 12 h, there were 134, 97, 114 and 159 genes up-regulated in the LPS, PGN, GLU and IC library, respectively. Most of the gene families involved in immune response towards PAMPs were ClqDCs, CTLs and FREPs, while only a few members of LRR and immunoglobin-containing proteins (LRRIGs), retinoic acid-inducible gene I [RIG-I]-like receptors (RLRs) and Toll like receptors (TLRs) were up-regulated. After LPS stimulation, the expression level of 258 non-redundant PRR genes in oyster haemocytes increased significantly with different expression pattern, and most of them were ClqDCs, CTLs, LRRops and FREPs. The transcriptomic analyses indicated that there was a dynamic and orchestrated specific expression regulation of numerous PRR genes ... Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 91 1 7 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Bivalve immunity Pattern recognition receptors Crassostrea gigas Transcriptome RNA-Seq Fisheries Immunology Zoology |
spellingShingle |
Bivalve immunity Pattern recognition receptors Crassostrea gigas Transcriptome RNA-Seq Fisheries Immunology Zoology Wang, Lingling Zhang, Huan Wang, Mengqiang Zhou, Zhi Wang, Weilin Liu, Rui Huang, Mengmeng Yang, Chuanyan Qiu, Limei Song, Linsheng The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas |
topic_facet |
Bivalve immunity Pattern recognition receptors Crassostrea gigas Transcriptome RNA-Seq Fisheries Immunology Zoology |
description |
Pattern recognition receptors (PRRs) are essential in recognizing specific pathogen-associated molecular patterns (PAMPs) on microbes and triggering responses to eliminate the invading pathogens. Previous genomic studies have revealed a great number of PRR genes in the Pacific oyster Crassostrea gigas, a sessile and filter-feeder marine bivalve belonging to the phylum Mollusca. On the survey of PRRs in the assembly oyster reference genome version 9, a total of 1084 PRRs were identified, which were composed of at least 12 gene families. Some of the gene families were significantly expanded, including C-type lectins (CTLs), fibrinogen-related proteins (FREPs), scavenger receptor cysteine-rich repeat protein (SRCRs), leucine-rich repeat (LRR)-only proteins (LRRops), and especially Clq domain-containing proteins (ClqDCs). The transcriptomic profiles of these abundant PRRs in response to PAMP treatments were investigated by RNA-Seq using the SOLiD EZ BeadTM system. Compared to the control library, there were 6,655, 7,273, 7,593, 6,830, 6687 and 8250 differentially expressed genes in the haemocytes of oysters in response to lipopolysaccharide (LPS) stimulation for 6 h, 12 h and 24 h, and peptidoglycan (PGN), glucan (GLU) and poly I:C (IC) stimulation for 12 h, respectively. After stimulation for 12 h, there were 134, 97, 114 and 159 genes up-regulated in the LPS, PGN, GLU and IC library, respectively. Most of the gene families involved in immune response towards PAMPs were ClqDCs, CTLs and FREPs, while only a few members of LRR and immunoglobin-containing proteins (LRRIGs), retinoic acid-inducible gene I [RIG-I]-like receptors (RLRs) and Toll like receptors (TLRs) were up-regulated. After LPS stimulation, the expression level of 258 non-redundant PRR genes in oyster haemocytes increased significantly with different expression pattern, and most of them were ClqDCs, CTLs, LRRops and FREPs. The transcriptomic analyses indicated that there was a dynamic and orchestrated specific expression regulation of numerous PRR genes ... |
format |
Report |
author |
Wang, Lingling Zhang, Huan Wang, Mengqiang Zhou, Zhi Wang, Weilin Liu, Rui Huang, Mengmeng Yang, Chuanyan Qiu, Limei Song, Linsheng |
author_facet |
Wang, Lingling Zhang, Huan Wang, Mengqiang Zhou, Zhi Wang, Weilin Liu, Rui Huang, Mengmeng Yang, Chuanyan Qiu, Limei Song, Linsheng |
author_sort |
Wang, Lingling |
title |
The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas |
title_short |
The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas |
title_full |
The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas |
title_fullStr |
The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas |
title_full_unstemmed |
The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas |
title_sort |
transcriptomic expression of pattern recognition receptors: insight into molecular recognition of various invading pathogens in oyster crassostrea gigas |
publisher |
ELSEVIER SCI LTD |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/156145 http://ir.qdio.ac.cn/handle/337002/156146 https://doi.org/10.1016/j.dci.2018.09.021 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/156145 http://ir.qdio.ac.cn/handle/337002/156146 doi:10.1016/j.dci.2018.09.021 |
op_doi |
https://doi.org/10.1016/j.dci.2018.09.021 |
container_title |
Developmental & Comparative Immunology |
container_volume |
91 |
container_start_page |
1 |
op_container_end_page |
7 |
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1766393777509892096 |