Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection
Megalocytivirus is a DNA virus with a broad host range among farmed fish including tongue sole (Cynoglossus semilaevis). In this study, label-free proteomics was performed to examine protein expression in tongue sole spleen induced by megalocytivirus at 8 and 12 days post infection (dpi). Compared t...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/155594 https://doi.org/10.1016/j.dci.2018.11.006 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/155594 2023-05-15T18:15:50+02:00 Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection Zhang, Jian Sun, Li 2019-03-01 http://ir.qdio.ac.cn/handle/337002/155594 https://doi.org/10.1016/j.dci.2018.11.006 英语 eng ELSEVIER SCI LTD DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/155594 doi:10.1016/j.dci.2018.11.006 Cynoglossus semilaevis Megalocytivirus Viral infection Immune defense Ubiquitin proteasome system Fisheries Immunology Zoology FLOUNDER PARALICHTHYS-OLIVACEUS TURBOT SCOPHTHALMUS-MAXIMUS IRIDOVIRUS-LIKE AGENT MANDARIN FISH SINIPERCA-CHUATSI JAPANESE FLOUNDER NECROSIS VIRUS ENDOSOMAL PROTEOLYSIS MANNOSE RECEPTOR CATHEPSIN-L 期刊论文 2019 ftchinacasciocas https://doi.org/10.1016/j.dci.2018.11.006 2022-06-27T05:38:46Z Megalocytivirus is a DNA virus with a broad host range among farmed fish including tongue sole (Cynoglossus semilaevis). In this study, label-free proteomics was performed to examine protein expression in tongue sole spleen induced by megalocytivirus at 8 and 12 days post infection (dpi). Compared to uninfected control fish, virus-infected fish displayed 315 up-regulated proteins and 111 down-regulated proteins at 8 dpi, and 48 up regulated proteins and 43 down-regulated proteins at 12 dpi. The expressions of five differentially expressed proteins were confirmed by Western blot. The differentially expressed proteins were enriched in the pathways and processes associated with innate immune response and viral infection. Interference with the expression of two up-regulated proteins of the ubiquitin proteasome system (UPS), i.e. proteasome assembly chaperone 2 and proteasome maturation protein, significantly reduced viral propagation in fish, whereas overexpression of these two proteins significantly enhanced viral propagation. Consistently, inhibition of the functioning of proteasome significantly impaired viral replication in vivo. This study provided the first global protein profile responsive to megalocytivirus in tongue sole, and revealed an essential role of UPS in viral infection. Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 92 150 159 |
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Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Cynoglossus semilaevis Megalocytivirus Viral infection Immune defense Ubiquitin proteasome system Fisheries Immunology Zoology FLOUNDER PARALICHTHYS-OLIVACEUS TURBOT SCOPHTHALMUS-MAXIMUS IRIDOVIRUS-LIKE AGENT MANDARIN FISH SINIPERCA-CHUATSI JAPANESE FLOUNDER NECROSIS VIRUS ENDOSOMAL PROTEOLYSIS MANNOSE RECEPTOR CATHEPSIN-L |
spellingShingle |
Cynoglossus semilaevis Megalocytivirus Viral infection Immune defense Ubiquitin proteasome system Fisheries Immunology Zoology FLOUNDER PARALICHTHYS-OLIVACEUS TURBOT SCOPHTHALMUS-MAXIMUS IRIDOVIRUS-LIKE AGENT MANDARIN FISH SINIPERCA-CHUATSI JAPANESE FLOUNDER NECROSIS VIRUS ENDOSOMAL PROTEOLYSIS MANNOSE RECEPTOR CATHEPSIN-L Zhang, Jian Sun, Li Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection |
topic_facet |
Cynoglossus semilaevis Megalocytivirus Viral infection Immune defense Ubiquitin proteasome system Fisheries Immunology Zoology FLOUNDER PARALICHTHYS-OLIVACEUS TURBOT SCOPHTHALMUS-MAXIMUS IRIDOVIRUS-LIKE AGENT MANDARIN FISH SINIPERCA-CHUATSI JAPANESE FLOUNDER NECROSIS VIRUS ENDOSOMAL PROTEOLYSIS MANNOSE RECEPTOR CATHEPSIN-L |
description |
Megalocytivirus is a DNA virus with a broad host range among farmed fish including tongue sole (Cynoglossus semilaevis). In this study, label-free proteomics was performed to examine protein expression in tongue sole spleen induced by megalocytivirus at 8 and 12 days post infection (dpi). Compared to uninfected control fish, virus-infected fish displayed 315 up-regulated proteins and 111 down-regulated proteins at 8 dpi, and 48 up regulated proteins and 43 down-regulated proteins at 12 dpi. The expressions of five differentially expressed proteins were confirmed by Western blot. The differentially expressed proteins were enriched in the pathways and processes associated with innate immune response and viral infection. Interference with the expression of two up-regulated proteins of the ubiquitin proteasome system (UPS), i.e. proteasome assembly chaperone 2 and proteasome maturation protein, significantly reduced viral propagation in fish, whereas overexpression of these two proteins significantly enhanced viral propagation. Consistently, inhibition of the functioning of proteasome significantly impaired viral replication in vivo. This study provided the first global protein profile responsive to megalocytivirus in tongue sole, and revealed an essential role of UPS in viral infection. |
format |
Report |
author |
Zhang, Jian Sun, Li |
author_facet |
Zhang, Jian Sun, Li |
author_sort |
Zhang, Jian |
title |
Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection |
title_short |
Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection |
title_full |
Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection |
title_fullStr |
Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection |
title_full_unstemmed |
Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection |
title_sort |
global profiling of megalocytivirus-induced proteins in tongue sole (cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection |
publisher |
ELSEVIER SCI LTD |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/155594 https://doi.org/10.1016/j.dci.2018.11.006 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/155594 doi:10.1016/j.dci.2018.11.006 |
op_doi |
https://doi.org/10.1016/j.dci.2018.11.006 |
container_title |
Developmental & Comparative Immunology |
container_volume |
92 |
container_start_page |
150 |
op_container_end_page |
159 |
_version_ |
1766189064541700096 |