Comparative study of beta-thymosin in two scallop species Argopecten irradians and Chlamys farreri

• Published in: Fish & Shellfish Immunology
• Main Authors: Wang, Mengqiang, Wang, Baojie, Liu, Mei, Jiang, Keyong, Wang, Lei
• Format: Report
• Language: English
• Published: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 2019
• Subjects: beta-thymosin; Innate immunity; Immunomodulation; Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences; OYSTER CRASSOSTREA-GIGAS; TRANSCRIPTOMIC RESPONSE; PATINOPECTEN-YESSOENSIS; IMMUNE-RESPONSE; COPPER EXPOSURE; EXPRESSION; GENES; IDENTIFICATION; PHAGOCYTOSIS; Crassostrea gigas
• Online Access: http://ir.qdio.ac.cn/handle/337002/155417; https://doi.org/10.1016/j.fsi.2018.11.050

The beta-thymosin (T beta) proteins participate in numerous biological processes, such as cell proliferation and differentiation, anti-inflammatory and antimicrobial mechanism. To date, T beta proteins have been well studied in vertebrates, especially mammals. While limited T beta or T beta-like proteins have been reported in invertebrates. Moreover, rare information of T beta or T beta-like proteins is available in scallop species yet. In the present study, two T beta homologues, AiT beta and CfT beta, were identified and characterized from two scallop species bay scallop Argopecten irradians and Zhikong scallop Chlamys farreri. They were both 41 amino acid peptide and contained one THY domain, a highly conserved actin-binding motif and two conserved helix forming regions. Tissue distribution and expression profiles of their mRNA transcripts were roughly similar yet different in detail, while their recombinant proteins exhibited different immunomodulation activity on the downstream immune parameters. These results collectively indicated that the function of T beta family in scallop were functionally differentiated.