Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors
In teleost, sex steroid hormones are critical for reproduction. Progestin is known to promote spermiation. To further understand the functions of progestin via its receptors during the annual reproductive cycle in male turbot (Scophthalmus maximus), we observed testicular development, quantified sev...
Published in: | Fish Physiology and Biochemistry |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/154338 https://doi.org/10.1007/s10695-017-0411-y |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/154338 2023-05-15T18:15:42+02:00 Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors Feng, Chengcheng Xu, Shihong Liu, Yifan Wang, Yanfeng Wang, Wenqi Yang, Jingkun Zhao, Chunyan Liu, Qinghua Li, Jun 2018-02-01 http://ir.qdio.ac.cn/handle/337002/154338 https://doi.org/10.1007/s10695-017-0411-y 英语 eng FISH PHYSIOLOGY AND BIOCHEMISTRY Feng, Chengcheng,Xu, Shihong,Liu, Yifan,et al. Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(1):35-48. http://ir.qdio.ac.cn/handle/337002/154338 doi:10.1007/s10695-017-0411-y Sex Steroid Hormones Progestin Receptors Testicular Development Sperm Motility Scophthalmus Maximus Article 期刊论文 2018 ftchinacasciocas https://doi.org/10.1007/s10695-017-0411-y 2022-06-27T05:38:16Z In teleost, sex steroid hormones are critical for reproduction. Progestin is known to promote spermiation. To further understand the functions of progestin via its receptors during the annual reproductive cycle in male turbot (Scophthalmus maximus), we observed testicular development, quantified several sex steroid hormones, detected the expression of progestin receptors, and measured various sperm parameters. Results showed that the turbot testicular structure was of the lobular type. During breeding season, a number of spermatocytes (stage III) developed into spermatids (stage IV), then differentiated into sperm during spermiogenesis (stage V), and finally regressed to spermatocytes (stage VI). Concomitant with testicular development, serum progesterone (P4) and 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (DHP) exhibited higher levels from stage IV to V than other stages. Furthermore, males with higher motility sperm showed higher levels of P4 and DHP compared with fish with lower motility sperm. These results indicated that P4 and DHP might induce spermatogenesis due to seasonal changes. Concurrently, in testes, the nuclear progesterone receptor (pgr) was expressed throughout the reproductive cycle and its level peaked during spermiogenesis while expression of membrane progestin receptor alpha (mPR alpha) did not change significantly. However, in sperm, mPR alpha expression was higher than in testes and had a significant positive correlation with curvilinear velocities (VCL), sperm motility, and motility duration. In conclusion, progestin appears to exert a direct pgr-mediated effect on spermiogenesis and improve sperm motility characteristics depending on the abundance of mPR alpha protein in sperm during spermiation. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish Physiology and Biochemistry 44 1 35 48 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Sex Steroid Hormones Progestin Receptors Testicular Development Sperm Motility Scophthalmus Maximus |
spellingShingle |
Sex Steroid Hormones Progestin Receptors Testicular Development Sperm Motility Scophthalmus Maximus Feng, Chengcheng Xu, Shihong Liu, Yifan Wang, Yanfeng Wang, Wenqi Yang, Jingkun Zhao, Chunyan Liu, Qinghua Li, Jun Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors |
topic_facet |
Sex Steroid Hormones Progestin Receptors Testicular Development Sperm Motility Scophthalmus Maximus |
description |
In teleost, sex steroid hormones are critical for reproduction. Progestin is known to promote spermiation. To further understand the functions of progestin via its receptors during the annual reproductive cycle in male turbot (Scophthalmus maximus), we observed testicular development, quantified several sex steroid hormones, detected the expression of progestin receptors, and measured various sperm parameters. Results showed that the turbot testicular structure was of the lobular type. During breeding season, a number of spermatocytes (stage III) developed into spermatids (stage IV), then differentiated into sperm during spermiogenesis (stage V), and finally regressed to spermatocytes (stage VI). Concomitant with testicular development, serum progesterone (P4) and 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (DHP) exhibited higher levels from stage IV to V than other stages. Furthermore, males with higher motility sperm showed higher levels of P4 and DHP compared with fish with lower motility sperm. These results indicated that P4 and DHP might induce spermatogenesis due to seasonal changes. Concurrently, in testes, the nuclear progesterone receptor (pgr) was expressed throughout the reproductive cycle and its level peaked during spermiogenesis while expression of membrane progestin receptor alpha (mPR alpha) did not change significantly. However, in sperm, mPR alpha expression was higher than in testes and had a significant positive correlation with curvilinear velocities (VCL), sperm motility, and motility duration. In conclusion, progestin appears to exert a direct pgr-mediated effect on spermiogenesis and improve sperm motility characteristics depending on the abundance of mPR alpha protein in sperm during spermiation. |
format |
Article in Journal/Newspaper |
author |
Feng, Chengcheng Xu, Shihong Liu, Yifan Wang, Yanfeng Wang, Wenqi Yang, Jingkun Zhao, Chunyan Liu, Qinghua Li, Jun |
author_facet |
Feng, Chengcheng Xu, Shihong Liu, Yifan Wang, Yanfeng Wang, Wenqi Yang, Jingkun Zhao, Chunyan Liu, Qinghua Li, Jun |
author_sort |
Feng, Chengcheng |
title |
Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors |
title_short |
Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors |
title_full |
Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors |
title_fullStr |
Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors |
title_full_unstemmed |
Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors |
title_sort |
progestin is important for testicular development of male turbot (scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/154338 https://doi.org/10.1007/s10695-017-0411-y |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FISH PHYSIOLOGY AND BIOCHEMISTRY Feng, Chengcheng,Xu, Shihong,Liu, Yifan,et al. Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(1):35-48. http://ir.qdio.ac.cn/handle/337002/154338 doi:10.1007/s10695-017-0411-y |
op_doi |
https://doi.org/10.1007/s10695-017-0411-y |
container_title |
Fish Physiology and Biochemistry |
container_volume |
44 |
container_issue |
1 |
container_start_page |
35 |
op_container_end_page |
48 |
_version_ |
1766188880775610368 |