Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining
Cytomorphological and cytochemical staining are important methods for the identification of cell types, in particular in fish which often lack biological tools such as specific antibodies. Myeloperoxidase (MPO) is usually used as an intracellular marker of neutrophil accumulation in tissues and a ma...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/143292 2023-05-15T18:41:08+02:00 Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining Chi, Heng Wen, Li-lian Sui, Zhi-Hai Sun, Qing-Lei Sun, Li 2017-12-01 http://ir.qdio.ac.cn/handle/337002/143292 https://doi.org/10.1016/j.tice.2017.10.008 英语 eng TISSUE & CELL Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,et al. Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining[J]. TISSUE & CELL,2017,49(6):751-755. http://ir.qdio.ac.cn/handle/337002/143292 doi:10.1016/j.tice.2017.10.008 Cytochemical Identification Ki-pyy Turbot Mpo-positive Granulocytes Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.tice.2017.10.008 2022-06-27T05:38:16Z Cytomorphological and cytochemical staining are important methods for the identification of cell types, in particular in fish which often lack biological tools such as specific antibodies. Myeloperoxidase (MPO) is usually used as an intracellular marker of neutrophil accumulation in tissues and a marker of neutrophil activity in plasma. In this study, we reported a potassium iodide and oxidized pyronine Y (KI-PyY) staining method for rapid and highly sensitive detection of MPO-positive cells in turbot blood, peritoneum, and tissues. MPO-positive cells, which mostly represented neutrophils, were stained brown and clearly distinguished from other cells, such as lymphocytes, monocytes, and macrophages, which were stained pink. Following bacterial stimulation, the proportions of neutrophils were 27.49% and 38.05% in peripheral blood leukocytes and peritoneum, respectively, judging by the stained MPO. Kidney granulocytes contained abundant MPO-positive cells which were probably immature neutrophils with low expression of MPO. It is noteworthy that MPO-positive cells were detected in the tissue sections of kidney, spleen, and gut, with distribution profiles specific to each tissue. However, the cell morphology was not distinct in the stained tissue sections. These results indicate that the KI-PyY staining method is highly sensitive, applicable to different types of samples, and will be useful for the study of neutrophils in different compartments of fish. Article in Journal/Newspaper Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Tissue and Cell 49 6 751 755 |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Cytochemical Identification Ki-pyy Turbot Mpo-positive Granulocytes |
spellingShingle |
Cytochemical Identification Ki-pyy Turbot Mpo-positive Granulocytes Chi, Heng Wen, Li-lian Sui, Zhi-Hai Sun, Qing-Lei Sun, Li Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining |
topic_facet |
Cytochemical Identification Ki-pyy Turbot Mpo-positive Granulocytes |
description |
Cytomorphological and cytochemical staining are important methods for the identification of cell types, in particular in fish which often lack biological tools such as specific antibodies. Myeloperoxidase (MPO) is usually used as an intracellular marker of neutrophil accumulation in tissues and a marker of neutrophil activity in plasma. In this study, we reported a potassium iodide and oxidized pyronine Y (KI-PyY) staining method for rapid and highly sensitive detection of MPO-positive cells in turbot blood, peritoneum, and tissues. MPO-positive cells, which mostly represented neutrophils, were stained brown and clearly distinguished from other cells, such as lymphocytes, monocytes, and macrophages, which were stained pink. Following bacterial stimulation, the proportions of neutrophils were 27.49% and 38.05% in peripheral blood leukocytes and peritoneum, respectively, judging by the stained MPO. Kidney granulocytes contained abundant MPO-positive cells which were probably immature neutrophils with low expression of MPO. It is noteworthy that MPO-positive cells were detected in the tissue sections of kidney, spleen, and gut, with distribution profiles specific to each tissue. However, the cell morphology was not distinct in the stained tissue sections. These results indicate that the KI-PyY staining method is highly sensitive, applicable to different types of samples, and will be useful for the study of neutrophils in different compartments of fish. |
format |
Article in Journal/Newspaper |
author |
Chi, Heng Wen, Li-lian Sui, Zhi-Hai Sun, Qing-Lei Sun, Li |
author_facet |
Chi, Heng Wen, Li-lian Sui, Zhi-Hai Sun, Qing-Lei Sun, Li |
author_sort |
Chi, Heng |
title |
Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining |
title_short |
Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining |
title_full |
Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining |
title_fullStr |
Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining |
title_full_unstemmed |
Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining |
title_sort |
cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine y staining |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/143292 https://doi.org/10.1016/j.tice.2017.10.008 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
TISSUE & CELL Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,et al. Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining[J]. TISSUE & CELL,2017,49(6):751-755. http://ir.qdio.ac.cn/handle/337002/143292 doi:10.1016/j.tice.2017.10.008 |
op_doi |
https://doi.org/10.1016/j.tice.2017.10.008 |
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Tissue and Cell |
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49 |
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6 |
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755 |
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1766230622810931200 |