Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus
Megalocytivirus is a severe pathogen to turbot (Scophthalmus maximus), a popular aquaculture species in many countries. In this study, we investigated the effect of temperature on the antiviral response of turbot at transcriptome level. We found that when turbot were infected with megalocytivirus RB...
Published in: | Fish & Shellfish Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/143216 2023-05-15T18:15:42+02:00 Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus Zhang, Jian Sun, Li 2017-09-01 http://ir.qdio.ac.cn/handle/337002/143216 https://doi.org/10.1016/j.fsi.2017.07.038 英语 eng FISH & SHELLFISH IMMUNOLOGY Zhang, Jian,Sun, Li. Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus[J]. FISH & SHELLFISH IMMUNOLOGY,2017,68:359-367. http://ir.qdio.ac.cn/handle/337002/143216 doi:10.1016/j.fsi.2017.07.038 Scophthalmus Maximus Transcriptome Viral Infection Immune Defense Cell Junctions Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.fsi.2017.07.038 2022-06-27T05:38:13Z Megalocytivirus is a severe pathogen to turbot (Scophthalmus maximus), a popular aquaculture species in many countries. In this study, we investigated the effect of temperature on the antiviral response of turbot at transcriptome level. We found that when turbot were infected with megalocytivirus RBIV-C1 at low temperatures (14 degrees C, 16 degrees C, and 18 degrees C), viral replication was undetectable or moderate and no fish mortality occurred; in contrast, when turbot were infected with RBIV-C1 at high temperatures (20 degrees C, 22 degrees C, and 24 degrees C), viral replication was robust and 100% host mortality was observed. During the course of viral infection, downward temperature shift curbed viral replication and augmented host survival, whereas upward temperature shift promoted viral replication and reduced host survival. Comparative transcriptome analyses were conducted to examine the whole-genome transcription of turbot infected with RBIV-C1 at 16 degrees C and 22 degrees C for 4 days (samples S16-4d and S22-4d, respectively) and 8 days (samples S16-8d and S22-8d, respectively). The results showed that compared to S22-4d and S22-8d, 1600 and 5927 upregulated unigenes of various functional categories were identified in S16-4d and S16-8d, respectively. Of these genes, 22 were immune-related, most of which were detected in S16-8d and exhibited more genetic subtypes in S16-8d than in S16-4d. In addition, upregulated genes associated with cell junctions and cell membrane were also identified. These results indicate that temperature had a profound effect on the global transcription of turbot, which consequently affects the immune as well as physical resistance of the fish against viral infection. (C) 2017 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 68 359 367 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Scophthalmus Maximus Transcriptome Viral Infection Immune Defense Cell Junctions |
spellingShingle |
Scophthalmus Maximus Transcriptome Viral Infection Immune Defense Cell Junctions Zhang, Jian Sun, Li Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus |
topic_facet |
Scophthalmus Maximus Transcriptome Viral Infection Immune Defense Cell Junctions |
description |
Megalocytivirus is a severe pathogen to turbot (Scophthalmus maximus), a popular aquaculture species in many countries. In this study, we investigated the effect of temperature on the antiviral response of turbot at transcriptome level. We found that when turbot were infected with megalocytivirus RBIV-C1 at low temperatures (14 degrees C, 16 degrees C, and 18 degrees C), viral replication was undetectable or moderate and no fish mortality occurred; in contrast, when turbot were infected with RBIV-C1 at high temperatures (20 degrees C, 22 degrees C, and 24 degrees C), viral replication was robust and 100% host mortality was observed. During the course of viral infection, downward temperature shift curbed viral replication and augmented host survival, whereas upward temperature shift promoted viral replication and reduced host survival. Comparative transcriptome analyses were conducted to examine the whole-genome transcription of turbot infected with RBIV-C1 at 16 degrees C and 22 degrees C for 4 days (samples S16-4d and S22-4d, respectively) and 8 days (samples S16-8d and S22-8d, respectively). The results showed that compared to S22-4d and S22-8d, 1600 and 5927 upregulated unigenes of various functional categories were identified in S16-4d and S16-8d, respectively. Of these genes, 22 were immune-related, most of which were detected in S16-8d and exhibited more genetic subtypes in S16-8d than in S16-4d. In addition, upregulated genes associated with cell junctions and cell membrane were also identified. These results indicate that temperature had a profound effect on the global transcription of turbot, which consequently affects the immune as well as physical resistance of the fish against viral infection. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Zhang, Jian Sun, Li |
author_facet |
Zhang, Jian Sun, Li |
author_sort |
Zhang, Jian |
title |
Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus |
title_short |
Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus |
title_full |
Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus |
title_fullStr |
Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus |
title_full_unstemmed |
Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus |
title_sort |
transcriptome analysis reveals temperature-regulated antiviral response in turbot scophthalmus maximus |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/143216 https://doi.org/10.1016/j.fsi.2017.07.038 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Zhang, Jian,Sun, Li. Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus[J]. FISH & SHELLFISH IMMUNOLOGY,2017,68:359-367. http://ir.qdio.ac.cn/handle/337002/143216 doi:10.1016/j.fsi.2017.07.038 |
op_doi |
https://doi.org/10.1016/j.fsi.2017.07.038 |
container_title |
Fish & Shellfish Immunology |
container_volume |
68 |
container_start_page |
359 |
op_container_end_page |
367 |
_version_ |
1766188880604692480 |