Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida
A rapid, economical, specific, and sensitive quantitative real-time polymerase chain reaction (qPCR) assay coupled with SYBR Green I chemistry was developed for the quantitative detection of Aeromonas salmonicida from farmed Atlantic salmon, Salmo salar, with the symptoms of furunculosis. The set of...
Published in: | Journal of the World Aquaculture Society |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/143089 2023-05-15T15:29:57+02:00 Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida Du, Yishuai Liu, Ying Xiao, Peng Meng, Lingjie Liu, Pengfei 2017-08-01 http://ir.qdio.ac.cn/handle/337002/143089 https://doi.org/10.1111/jwas.12395 英语 eng JOURNAL OF THE WORLD AQUACULTURE SOCIETY Du, Yishuai,Liu, Ying,Xiao, Peng,et al. Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(4):574-582. http://ir.qdio.ac.cn/handle/337002/143089 doi:10.1111/jwas.12395 Atlantic Salmon Aeromonas Salmonicida Real-time Pcr Sybr Green i Quantitative Detection Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1111/jwas.12395 2022-06-27T05:38:10Z A rapid, economical, specific, and sensitive quantitative real-time polymerase chain reaction (qPCR) assay coupled with SYBR Green I chemistry was developed for the quantitative detection of Aeromonas salmonicida from farmed Atlantic salmon, Salmo salar, with the symptoms of furunculosis. The set of primers designed from the virulence array protein (vapA) gene was specific to A. salmonicida. Compared with the conventional PCR, qPCR had a lower detection limit of 5.6 copies of the positive plasmids. The standard curve, which showed the relationship between the copies of A. salmonicida and its quantification cycle (C-q) value, could be described as follows: log (copies of A. salmonicida)=-0.3213 C-q + 10.721. The quantitative detection of copies of A. salmonicida in different tissues of the moribund Atlantic salmon showed that A. salmonicida could be detected in all tissues; the spleen contained the largest number of A. salmonicida and then the kidney. These results suggest that the qPCR assay reported here is a specific, sensitive, and quantitative method for detecting A. salmonicida. It can be used for the routine tests of A. salmonicida in local aquaculture enterprise and for the research of infection routes of A. salmonicida to Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Journal of the World Aquaculture Society 48 4 574 582 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Atlantic Salmon Aeromonas Salmonicida Real-time Pcr Sybr Green i Quantitative Detection |
spellingShingle |
Atlantic Salmon Aeromonas Salmonicida Real-time Pcr Sybr Green i Quantitative Detection Du, Yishuai Liu, Ying Xiao, Peng Meng, Lingjie Liu, Pengfei Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida |
topic_facet |
Atlantic Salmon Aeromonas Salmonicida Real-time Pcr Sybr Green i Quantitative Detection |
description |
A rapid, economical, specific, and sensitive quantitative real-time polymerase chain reaction (qPCR) assay coupled with SYBR Green I chemistry was developed for the quantitative detection of Aeromonas salmonicida from farmed Atlantic salmon, Salmo salar, with the symptoms of furunculosis. The set of primers designed from the virulence array protein (vapA) gene was specific to A. salmonicida. Compared with the conventional PCR, qPCR had a lower detection limit of 5.6 copies of the positive plasmids. The standard curve, which showed the relationship between the copies of A. salmonicida and its quantification cycle (C-q) value, could be described as follows: log (copies of A. salmonicida)=-0.3213 C-q + 10.721. The quantitative detection of copies of A. salmonicida in different tissues of the moribund Atlantic salmon showed that A. salmonicida could be detected in all tissues; the spleen contained the largest number of A. salmonicida and then the kidney. These results suggest that the qPCR assay reported here is a specific, sensitive, and quantitative method for detecting A. salmonicida. It can be used for the routine tests of A. salmonicida in local aquaculture enterprise and for the research of infection routes of A. salmonicida to Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Du, Yishuai Liu, Ying Xiao, Peng Meng, Lingjie Liu, Pengfei |
author_facet |
Du, Yishuai Liu, Ying Xiao, Peng Meng, Lingjie Liu, Pengfei |
author_sort |
Du, Yishuai |
title |
Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida |
title_short |
Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida |
title_full |
Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida |
title_fullStr |
Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida |
title_full_unstemmed |
Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida |
title_sort |
development and application of a quantitative real-time polymerase chain reaction assay for the detection of aeromonas salmonicida |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/143089 https://doi.org/10.1111/jwas.12395 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
JOURNAL OF THE WORLD AQUACULTURE SOCIETY Du, Yishuai,Liu, Ying,Xiao, Peng,et al. Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(4):574-582. http://ir.qdio.ac.cn/handle/337002/143089 doi:10.1111/jwas.12395 |
op_doi |
https://doi.org/10.1111/jwas.12395 |
container_title |
Journal of the World Aquaculture Society |
container_volume |
48 |
container_issue |
4 |
container_start_page |
574 |
op_container_end_page |
582 |
_version_ |
1766360397874462720 |