The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas
The complement system is one of the major effector mechanisms of immune system, playing essential roles in both the innate and adaptive immune responses. In the present study, the counterparts of vertebrate complement components were identified by screening the sequenced genome of Crassostrea gigas,...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/143083 2023-05-15T15:57:44+02:00 The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas Wang, Lingling Zhang, Huan Wang, Leilei Zhang, Daoxiang Lv, Zhao Liu, Zhaoqun Wang, Weilin Zhou, Zhi Qiu, Limei Wang, Hao Li, Jun Song, Linsheng 2017-11-01 http://ir.qdio.ac.cn/handle/337002/143083 https://doi.org/10.1016/j.dci.2017.06.009 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Wang, Lingling,Zhang, Huan,Wang, Leilei,et al. The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,76:209-219. http://ir.qdio.ac.cn/handle/337002/143083 doi:10.1016/j.dci.2017.06.009 Crassostrea Gigas Complement Related Prrs Serine Protease Domain-containing Proteins C3 C3 Receptor-like Proteins Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.dci.2017.06.009 2022-06-27T05:38:10Z The complement system is one of the major effector mechanisms of immune system, playing essential roles in both the innate and adaptive immune responses. In the present study, the counterparts of vertebrate complement components were identified by screening the sequenced genome of Crassostrea gigas, resulting in the identification of 792 gene models containing complement-related domains. The transcriptome of haemocytes at 6, 12 and 24 h post lipopolysaccharides (LPS) stimulation showed differential expression of 77 Clq domain containing proteins, 53 C-type lectins and 42 fibrinogen-related proteins. mRNAs encoding 18 serine protease domain-containing (SPC) proteins, 4 MACPF-domain containing proteins and 11 C3 receptor-like proteins were up-regulated upon LPS stimulation, and CgC3 mRNA was significantly increased at 12 h. The presence of CgC3 was confirmed in cell free plasma and was present in three subunit chains as expected for the processed mature protein. The complement related PRRs with coiled coil regions and SPC proteins with CUB domains may function in the activation of CgC3, whereas, the C3-like receptors with integrin-alpha/beta domain mediated the phagocytosis of C3-labled pathogens. These PRRs appear to serve as opsonins to promote phagocytosis of opsonized pathogens. The overall results suggested the existence of a potential multi-component complement system in C gigas. (C) 2017 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 76 209 219 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Complement Related Prrs Serine Protease Domain-containing Proteins C3 C3 Receptor-like Proteins |
spellingShingle |
Crassostrea Gigas Complement Related Prrs Serine Protease Domain-containing Proteins C3 C3 Receptor-like Proteins Wang, Lingling Zhang, Huan Wang, Leilei Zhang, Daoxiang Lv, Zhao Liu, Zhaoqun Wang, Weilin Zhou, Zhi Qiu, Limei Wang, Hao Li, Jun Song, Linsheng The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas |
topic_facet |
Crassostrea Gigas Complement Related Prrs Serine Protease Domain-containing Proteins C3 C3 Receptor-like Proteins |
description |
The complement system is one of the major effector mechanisms of immune system, playing essential roles in both the innate and adaptive immune responses. In the present study, the counterparts of vertebrate complement components were identified by screening the sequenced genome of Crassostrea gigas, resulting in the identification of 792 gene models containing complement-related domains. The transcriptome of haemocytes at 6, 12 and 24 h post lipopolysaccharides (LPS) stimulation showed differential expression of 77 Clq domain containing proteins, 53 C-type lectins and 42 fibrinogen-related proteins. mRNAs encoding 18 serine protease domain-containing (SPC) proteins, 4 MACPF-domain containing proteins and 11 C3 receptor-like proteins were up-regulated upon LPS stimulation, and CgC3 mRNA was significantly increased at 12 h. The presence of CgC3 was confirmed in cell free plasma and was present in three subunit chains as expected for the processed mature protein. The complement related PRRs with coiled coil regions and SPC proteins with CUB domains may function in the activation of CgC3, whereas, the C3-like receptors with integrin-alpha/beta domain mediated the phagocytosis of C3-labled pathogens. These PRRs appear to serve as opsonins to promote phagocytosis of opsonized pathogens. The overall results suggested the existence of a potential multi-component complement system in C gigas. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Wang, Lingling Zhang, Huan Wang, Leilei Zhang, Daoxiang Lv, Zhao Liu, Zhaoqun Wang, Weilin Zhou, Zhi Qiu, Limei Wang, Hao Li, Jun Song, Linsheng |
author_facet |
Wang, Lingling Zhang, Huan Wang, Leilei Zhang, Daoxiang Lv, Zhao Liu, Zhaoqun Wang, Weilin Zhou, Zhi Qiu, Limei Wang, Hao Li, Jun Song, Linsheng |
author_sort |
Wang, Lingling |
title |
The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas |
title_short |
The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas |
title_full |
The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas |
title_fullStr |
The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas |
title_full_unstemmed |
The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas |
title_sort |
rna-seq analysis suggests a potential multi-component complement system in oyster crassostrea gigas |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/143083 https://doi.org/10.1016/j.dci.2017.06.009 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Wang, Lingling,Zhang, Huan,Wang, Leilei,et al. The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,76:209-219. http://ir.qdio.ac.cn/handle/337002/143083 doi:10.1016/j.dci.2017.06.009 |
op_doi |
https://doi.org/10.1016/j.dci.2017.06.009 |
container_title |
Developmental & Comparative Immunology |
container_volume |
76 |
container_start_page |
209 |
op_container_end_page |
219 |
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1766393433082036224 |