Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT
C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/143079 https://doi.org/10.1016/j.dci.2017.09.014 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/143079 2023-05-15T15:59:06+02:00 Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT Lv, Zhao Qiu, Limei Wang, Mengqiang Jia, Zhihao Wang, Weilin Xin, Lusheng Liu, Zhaoqun Wang, Lingling Song, Linsheng 2018 http://ir.qdio.ac.cn/handle/337002/143079 https://doi.org/10.1016/j.dci.2017.09.014 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Lv, Zhao,Qiu, Limei,Wang, Mengqiang,et al. Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,78:42-51. http://ir.qdio.ac.cn/handle/337002/143079 doi:10.1016/j.dci.2017.09.014 C1qdc Proteins Gram-negative Bacteria Recognition Specificity Lps Binding Affinity Article 期刊论文 2018 ftchinacasciocas https://doi.org/10.1016/j.dci.2017.09.014 2022-06-27T05:38:10Z C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas(CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind Iipoteichoic acid (LTA), beta-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Correspondingly, they all exhibited higher binding activities towards Gram-negative bacteria Vibrio anguillarum and V. splendidus. Moreover, they could enhance the phagocytosis of oyster hemocytes, and the enhancements towards Gram-negative bacteria were significantly higher than that towards Gram-positive bacteria (p < 0.01). The LPS binding affinity of rCgC1qDC-3 (K-D = 8.74 x 10(-7) M) was higher than that of rCgC1qDC-2 (K-D = 7.76 x 10(-5) M) and rCgC1qDC-4 (K-D = 1.09 x 10(-5) M). Meanwhile, rCgC1qDC-3 exhibited significantly higher enhancement on phagocytosis of oyster hemocytes towards Gram-negative bacteria than that of rCgC1qDC-2 and rCgC1qDC-4 (p < 0.05). After the secondary challenge with V. splendidus, the up-regulations of CgC1qDC-2 and CgC1qDC-4 mRNA in hemocytes occurred at 6 h, while that of CgC1qDC-3 was observed at 3 h and lasted for 24 h. And CgC1qDC-3 responded with high mRNA level for tested 24 h upon the secondary challenge with V. anguillarum as well. These results collectively suggested that three CgC1qDCs could serve as PRRs to specifically recognize certain Gram-negative bacteria and opsonins to enhance phagocytosis. CgC1qDC-3, with higher binding affinity to LPS, stronger opsonization and more rapid and persistent mRNA expression response upon the secondary challenge with homologous Vibrios, might exert efficient ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 78 42 51 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
C1qdc Proteins Gram-negative Bacteria Recognition Specificity Lps Binding Affinity |
spellingShingle |
C1qdc Proteins Gram-negative Bacteria Recognition Specificity Lps Binding Affinity Lv, Zhao Qiu, Limei Wang, Mengqiang Jia, Zhihao Wang, Weilin Xin, Lusheng Liu, Zhaoqun Wang, Lingling Song, Linsheng Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT |
topic_facet |
C1qdc Proteins Gram-negative Bacteria Recognition Specificity Lps Binding Affinity |
description |
C1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas(CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind Iipoteichoic acid (LTA), beta-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Correspondingly, they all exhibited higher binding activities towards Gram-negative bacteria Vibrio anguillarum and V. splendidus. Moreover, they could enhance the phagocytosis of oyster hemocytes, and the enhancements towards Gram-negative bacteria were significantly higher than that towards Gram-positive bacteria (p < 0.01). The LPS binding affinity of rCgC1qDC-3 (K-D = 8.74 x 10(-7) M) was higher than that of rCgC1qDC-2 (K-D = 7.76 x 10(-5) M) and rCgC1qDC-4 (K-D = 1.09 x 10(-5) M). Meanwhile, rCgC1qDC-3 exhibited significantly higher enhancement on phagocytosis of oyster hemocytes towards Gram-negative bacteria than that of rCgC1qDC-2 and rCgC1qDC-4 (p < 0.05). After the secondary challenge with V. splendidus, the up-regulations of CgC1qDC-2 and CgC1qDC-4 mRNA in hemocytes occurred at 6 h, while that of CgC1qDC-3 was observed at 3 h and lasted for 24 h. And CgC1qDC-3 responded with high mRNA level for tested 24 h upon the secondary challenge with V. anguillarum as well. These results collectively suggested that three CgC1qDCs could serve as PRRs to specifically recognize certain Gram-negative bacteria and opsonins to enhance phagocytosis. CgC1qDC-3, with higher binding affinity to LPS, stronger opsonization and more rapid and persistent mRNA expression response upon the secondary challenge with homologous Vibrios, might exert efficient ... |
format |
Article in Journal/Newspaper |
author |
Lv, Zhao Qiu, Limei Wang, Mengqiang Jia, Zhihao Wang, Weilin Xin, Lusheng Liu, Zhaoqun Wang, Lingling Song, Linsheng |
author_facet |
Lv, Zhao Qiu, Limei Wang, Mengqiang Jia, Zhihao Wang, Weilin Xin, Lusheng Liu, Zhaoqun Wang, Lingling Song, Linsheng |
author_sort |
Lv, Zhao |
title |
Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT |
title_short |
Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT |
title_full |
Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT |
title_fullStr |
Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT |
title_full_unstemmed |
Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT |
title_sort |
comparative study of three c1q domain containing proteins from pacific oyster &itcrassostrea gigas&it |
publishDate |
2018 |
url |
http://ir.qdio.ac.cn/handle/337002/143079 https://doi.org/10.1016/j.dci.2017.09.014 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Lv, Zhao,Qiu, Limei,Wang, Mengqiang,et al. Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,78:42-51. http://ir.qdio.ac.cn/handle/337002/143079 doi:10.1016/j.dci.2017.09.014 |
op_doi |
https://doi.org/10.1016/j.dci.2017.09.014 |
container_title |
Developmental & Comparative Immunology |
container_volume |
78 |
container_start_page |
42 |
op_container_end_page |
51 |
_version_ |
1766394879136497664 |