Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway
Interleukin-1 receptor-associated kinases (IRAKs) play important roles in MyD88-dependent TLR signaling, the crucial innate immune pathway in molluscs. In this study, we examined the full-length IRAK4 genetic sequence in the Pacific oyster (Crassostrea gigas) by molecular cloning. Phylogenetic analy...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/137057 2023-05-15T15:57:35+02:00 Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan 2017-07-01 http://ir.qdio.ac.cn/handle/337002/137057 https://doi.org/10.1016/j.dci.2017.02.004 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Tang, Xueying,Huang, Baoyu,Zhang, Linlin,et al. Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,72:21-29. http://ir.qdio.ac.cn/handle/337002/137057 doi:10.1016/j.dci.2017.02.004 Crassostrea Gigas Irak4 Myd88 Tlr Nf-kappa b Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.dci.2017.02.004 2022-06-27T05:38:04Z Interleukin-1 receptor-associated kinases (IRAKs) play important roles in MyD88-dependent TLR signaling, the crucial innate immune pathway in molluscs. In this study, we examined the full-length IRAK4 genetic sequence in the Pacific oyster (Crassostrea gigas) by molecular cloning. Phylogenetic analysis revealed that CgIRAK4 is most closely related to Mytilus edulis, and forms a Glade with other molluscs. CgIRAK4 transcripts are widely expressed in all tissues, with the highest expression observed in the hemocytes and gill. Moreover, CgIRAK4 is significantly upregulated after Oyster herpesvirus-1 microvariant (0sHV-1 mu var), Vibrio alginolyticus, and poly I:C challenge. Yeast two-hybrid and co-immunoprecipitation assays reveal that the CgIRAK4 death domain is necessary to mediate interaction between CgIRAK4 and two CgMyD88 isoforms. In addition, CgIRAK4 overexpression cannot induce NF-kappa B transcriptional activity, but blocks that induced by CgMyD88 in HEK293T cells. These findings elucidate the mechanisms of MyD88-dependent TLR signaling in molluscs, and the differences in IRAK-mediated pathway activation between invertebrates and vertebrates. (C) 2017 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 72 21 29 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Irak4 Myd88 Tlr Nf-kappa b |
spellingShingle |
Crassostrea Gigas Irak4 Myd88 Tlr Nf-kappa b Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway |
topic_facet |
Crassostrea Gigas Irak4 Myd88 Tlr Nf-kappa b |
description |
Interleukin-1 receptor-associated kinases (IRAKs) play important roles in MyD88-dependent TLR signaling, the crucial innate immune pathway in molluscs. In this study, we examined the full-length IRAK4 genetic sequence in the Pacific oyster (Crassostrea gigas) by molecular cloning. Phylogenetic analysis revealed that CgIRAK4 is most closely related to Mytilus edulis, and forms a Glade with other molluscs. CgIRAK4 transcripts are widely expressed in all tissues, with the highest expression observed in the hemocytes and gill. Moreover, CgIRAK4 is significantly upregulated after Oyster herpesvirus-1 microvariant (0sHV-1 mu var), Vibrio alginolyticus, and poly I:C challenge. Yeast two-hybrid and co-immunoprecipitation assays reveal that the CgIRAK4 death domain is necessary to mediate interaction between CgIRAK4 and two CgMyD88 isoforms. In addition, CgIRAK4 overexpression cannot induce NF-kappa B transcriptional activity, but blocks that induced by CgMyD88 in HEK293T cells. These findings elucidate the mechanisms of MyD88-dependent TLR signaling in molluscs, and the differences in IRAK-mediated pathway activation between invertebrates and vertebrates. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan |
author_facet |
Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan |
author_sort |
Tang, Xueying |
title |
Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway |
title_short |
Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway |
title_full |
Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway |
title_fullStr |
Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway |
title_full_unstemmed |
Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway |
title_sort |
molecular characterization of pacific oyster (crassostrea gigas) irak4 gene and its role in myd88-dependent pathway |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/137057 https://doi.org/10.1016/j.dci.2017.02.004 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Tang, Xueying,Huang, Baoyu,Zhang, Linlin,et al. Molecular characterization of Pacific oyster (Crassostrea gigas) IRAK4 gene and its role in MyD88-dependent pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,72:21-29. http://ir.qdio.ac.cn/handle/337002/137057 doi:10.1016/j.dci.2017.02.004 |
op_doi |
https://doi.org/10.1016/j.dci.2017.02.004 |
container_title |
Developmental & Comparative Immunology |
container_volume |
72 |
container_start_page |
21 |
op_container_end_page |
29 |
_version_ |
1766393254865010688 |