The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture
Pathogen contamination in the environment is inevitable with the rapid development of intensive aquaculture. Therefore, alternative ecofriendly biological strategies to control pathogenic bacteria are required. However, our aim was to investigate the ability of oysters (Crassostrea gigas) to filter...
Published in: | Applied Microbiology and Biotechnology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/137033 2023-05-15T15:32:50+02:00 The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture Ma, Xiaona Li, Xian Sun, Guoxiang Sharawy, Zaki Zaki Qiu, Tianlong Du, Yishuai Liu, Ying 2017-07-01 http://ir.qdio.ac.cn/handle/337002/137033 https://doi.org/10.1007/s00253-017-8296-3 英语 eng APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Ma, Xiaona,Li, Xian,Sun, Guoxiang,et al. The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(14):5869-5880. http://ir.qdio.ac.cn/handle/337002/137033 doi:10.1007/s00253-017-8296-3 Bacterial Control Aeromonas Salmonicida Oysters Biofilter Wastewater Treatments Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1007/s00253-017-8296-3 2022-06-27T05:38:04Z Pathogen contamination in the environment is inevitable with the rapid development of intensive aquaculture. Therefore, alternative ecofriendly biological strategies to control pathogenic bacteria are required. However, our aim was to investigate the ability of oysters (Crassostrea gigas) to filter the important opportunistic pathogen, Aeromonas salmonicida (strain C4), using a green fluorescent protein tag (GFP) in the Atlantic salmon (Salmo salar) farming wastewater. Hence, A. salmonicida removal efficiency and ingestion rate were detected in two different oyster stages (larvae and adults). To evaluate the practical performance of oysters as A. salmonicida biofilter, adult oysters were applied to an integrated constructed wetlands system (ICWS) and their long-term C4-GFP removal efficiency was recorded for 60 days. Overall, our results clearly indicated that oysters had substantial A. salmonicida removal ability via their ingestion process when observed under a fluorescent microscope. Approximately 88-95% of C4-GFP was removed by oyster larvae at an ingestion rate of 6.4 x 10(3)-6.2 x 10(5) CFU/h center dot ind, while 79-92% of C4-GFP was removed by adult oysters at an ingestion rate of 2.1 x 10(4)-3.1 x 10(6) CFU/h center dot ind. Furthermore, 57.9 +/- 17.2% of C4-GFP removal efficiency was achieved when oysters were applied to ICWS. We, therefore, concluded that using oysters as a biofilter represents an effective alternative for removing A. salmonicida from aquaculture wastewater. However, the fate of oysters after ingesting the pathogenic bacteria, acting as a potential reservoir or vector for pathogens, is still debatable. This research provides the basis for the application of oysters as a biofilter to remove pathogens from aquaculture wastewater in industrialized production. Article in Journal/Newspaper Atlantic salmon Crassostrea gigas Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Applied Microbiology and Biotechnology 101 14 5869 5880 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Bacterial Control Aeromonas Salmonicida Oysters Biofilter Wastewater Treatments |
spellingShingle |
Bacterial Control Aeromonas Salmonicida Oysters Biofilter Wastewater Treatments Ma, Xiaona Li, Xian Sun, Guoxiang Sharawy, Zaki Zaki Qiu, Tianlong Du, Yishuai Liu, Ying The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture |
topic_facet |
Bacterial Control Aeromonas Salmonicida Oysters Biofilter Wastewater Treatments |
description |
Pathogen contamination in the environment is inevitable with the rapid development of intensive aquaculture. Therefore, alternative ecofriendly biological strategies to control pathogenic bacteria are required. However, our aim was to investigate the ability of oysters (Crassostrea gigas) to filter the important opportunistic pathogen, Aeromonas salmonicida (strain C4), using a green fluorescent protein tag (GFP) in the Atlantic salmon (Salmo salar) farming wastewater. Hence, A. salmonicida removal efficiency and ingestion rate were detected in two different oyster stages (larvae and adults). To evaluate the practical performance of oysters as A. salmonicida biofilter, adult oysters were applied to an integrated constructed wetlands system (ICWS) and their long-term C4-GFP removal efficiency was recorded for 60 days. Overall, our results clearly indicated that oysters had substantial A. salmonicida removal ability via their ingestion process when observed under a fluorescent microscope. Approximately 88-95% of C4-GFP was removed by oyster larvae at an ingestion rate of 6.4 x 10(3)-6.2 x 10(5) CFU/h center dot ind, while 79-92% of C4-GFP was removed by adult oysters at an ingestion rate of 2.1 x 10(4)-3.1 x 10(6) CFU/h center dot ind. Furthermore, 57.9 +/- 17.2% of C4-GFP removal efficiency was achieved when oysters were applied to ICWS. We, therefore, concluded that using oysters as a biofilter represents an effective alternative for removing A. salmonicida from aquaculture wastewater. However, the fate of oysters after ingesting the pathogenic bacteria, acting as a potential reservoir or vector for pathogens, is still debatable. This research provides the basis for the application of oysters as a biofilter to remove pathogens from aquaculture wastewater in industrialized production. |
format |
Article in Journal/Newspaper |
author |
Ma, Xiaona Li, Xian Sun, Guoxiang Sharawy, Zaki Zaki Qiu, Tianlong Du, Yishuai Liu, Ying |
author_facet |
Ma, Xiaona Li, Xian Sun, Guoxiang Sharawy, Zaki Zaki Qiu, Tianlong Du, Yishuai Liu, Ying |
author_sort |
Ma, Xiaona |
title |
The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture |
title_short |
The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture |
title_full |
The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture |
title_fullStr |
The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture |
title_full_unstemmed |
The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture |
title_sort |
biofilteration ability of oysters (crassostrea gigas) to reduce aeromonas salmonicida in salmon culture |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/137033 https://doi.org/10.1007/s00253-017-8296-3 |
genre |
Atlantic salmon Crassostrea gigas Salmo salar |
genre_facet |
Atlantic salmon Crassostrea gigas Salmo salar |
op_relation |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Ma, Xiaona,Li, Xian,Sun, Guoxiang,et al. The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(14):5869-5880. http://ir.qdio.ac.cn/handle/337002/137033 doi:10.1007/s00253-017-8296-3 |
op_doi |
https://doi.org/10.1007/s00253-017-8296-3 |
container_title |
Applied Microbiology and Biotechnology |
container_volume |
101 |
container_issue |
14 |
container_start_page |
5869 |
op_container_end_page |
5880 |
_version_ |
1766363322415841280 |