Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress
Bacterial infection and heat stress, as two major environmental threats of marine molluscs, could affect larval development and dramatically promote mortality of oysters. In the present study, next-generation sequencing, together with determinations of mRNA expression and measurements of enzyme acti...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136861 2023-05-15T15:57:44+02:00 Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress Liu, Zhaoqun Wang, Lingling Zhou, Zhi Liu, Yu Dong, Miren Wang, Weilin Song, Xiaorui Wang, Mengqiang Gao, Qiang Song, Linsheng 2017-08-01 http://ir.qdio.ac.cn/handle/337002/136861 https://doi.org/10.1016/j.dci.2017.03.005 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Liu, Zhaoqun,Wang, Lingling,Zhou, Zhi,et al. Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:52-60. http://ir.qdio.ac.cn/handle/337002/136861 doi:10.1016/j.dci.2017.03.005 Crassostrea Gigas Larvae Transcriptomic Analysis Acute Heat And Bacterial Stress Catecholaminergic System Immunomodulation Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.dci.2017.03.005 2022-06-27T05:38:04Z Bacterial infection and heat stress, as two major environmental threats of marine molluscs, could affect larval development and dramatically promote mortality of oysters. In the present study, next-generation sequencing, together with determinations of mRNA expression and measurements of enzyme activities, were employed to understand the response patterns of oyster larvae under acute heat and bacterial stress. After RNA-seq, a total of 9472 differentially expressed genes including 4895 significantly up regulated ones and 4577 significantly down-regulated ones were obtained from 12 transcriptome libraries. GO overrepresentation analysis of the up-regulated genes revealed that the neuroendocrine immunomodulation pathway was activated after acute heat and bacterial stimulation, in which the catecholaminergic regulation played an important role. GO overrepresentation analysis of the down regulated genes suggested that the immune capacity of Crassostrea gigas larvae was suppressed under stress, which was further validated since superoxide dismutase (SOD) and phenoloxidase (PO) activities in the total protein extract of larvae decreased dramatically after stress. Moreover, the shell formation of trochophore was inhibited and severe mortality was caused after acute heat and bacterial stress. These results collectively indicated that acute heat and bacterial stress could significantly inhibit larval development and suppress immune response of oyster C. gigas larvae. And the neuroendocrine immunomodulation, especially the catecholaminergic regulation, played an indispensable role in the stress response of molluscan larvae. (C) 2017 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 73 52 60 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Larvae Transcriptomic Analysis Acute Heat And Bacterial Stress Catecholaminergic System Immunomodulation |
spellingShingle |
Crassostrea Gigas Larvae Transcriptomic Analysis Acute Heat And Bacterial Stress Catecholaminergic System Immunomodulation Liu, Zhaoqun Wang, Lingling Zhou, Zhi Liu, Yu Dong, Miren Wang, Weilin Song, Xiaorui Wang, Mengqiang Gao, Qiang Song, Linsheng Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress |
topic_facet |
Crassostrea Gigas Larvae Transcriptomic Analysis Acute Heat And Bacterial Stress Catecholaminergic System Immunomodulation |
description |
Bacterial infection and heat stress, as two major environmental threats of marine molluscs, could affect larval development and dramatically promote mortality of oysters. In the present study, next-generation sequencing, together with determinations of mRNA expression and measurements of enzyme activities, were employed to understand the response patterns of oyster larvae under acute heat and bacterial stress. After RNA-seq, a total of 9472 differentially expressed genes including 4895 significantly up regulated ones and 4577 significantly down-regulated ones were obtained from 12 transcriptome libraries. GO overrepresentation analysis of the up-regulated genes revealed that the neuroendocrine immunomodulation pathway was activated after acute heat and bacterial stimulation, in which the catecholaminergic regulation played an important role. GO overrepresentation analysis of the down regulated genes suggested that the immune capacity of Crassostrea gigas larvae was suppressed under stress, which was further validated since superoxide dismutase (SOD) and phenoloxidase (PO) activities in the total protein extract of larvae decreased dramatically after stress. Moreover, the shell formation of trochophore was inhibited and severe mortality was caused after acute heat and bacterial stress. These results collectively indicated that acute heat and bacterial stress could significantly inhibit larval development and suppress immune response of oyster C. gigas larvae. And the neuroendocrine immunomodulation, especially the catecholaminergic regulation, played an indispensable role in the stress response of molluscan larvae. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Liu, Zhaoqun Wang, Lingling Zhou, Zhi Liu, Yu Dong, Miren Wang, Weilin Song, Xiaorui Wang, Mengqiang Gao, Qiang Song, Linsheng |
author_facet |
Liu, Zhaoqun Wang, Lingling Zhou, Zhi Liu, Yu Dong, Miren Wang, Weilin Song, Xiaorui Wang, Mengqiang Gao, Qiang Song, Linsheng |
author_sort |
Liu, Zhaoqun |
title |
Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress |
title_short |
Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress |
title_full |
Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress |
title_fullStr |
Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress |
title_full_unstemmed |
Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress |
title_sort |
transcriptomic analysis of oyster crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/136861 https://doi.org/10.1016/j.dci.2017.03.005 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Liu, Zhaoqun,Wang, Lingling,Zhou, Zhi,et al. Transcriptomic analysis of oyster Crassostrea gigas larvae illustrates the response patterns regulated by catecholaminergic system upon acute heat and bacterial stress[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:52-60. http://ir.qdio.ac.cn/handle/337002/136861 doi:10.1016/j.dci.2017.03.005 |
op_doi |
https://doi.org/10.1016/j.dci.2017.03.005 |
container_title |
Developmental & Comparative Immunology |
container_volume |
73 |
container_start_page |
52 |
op_container_end_page |
60 |
_version_ |
1766393432911118336 |