The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response

B-cell translocation gene 1 (BTG1) is a member of the anti-proliferative gene family, which plays important roles in regulation of cell cycle. In the present study, a B-cell translocation gene 1 molecule homologue (designed CgBTG1) are identified and characterized in oyster Crassostrea gigas. CgBTG1...

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Published in:Fish & Shellfish Immunology
Main Authors: Liu, Rui, Cheng, Qi, Wang, Xiudan, Chen, Hao, Wang, Weilin, Zhang, Huan, Wang, Lingling, Song, Linsheng
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Online Access:http://ir.qdio.ac.cn/handle/337002/136789
https://doi.org/10.1016/j.fsi.2016.12.005
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spelling ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136789 2023-05-15T15:57:47+02:00 The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response Liu, Rui Cheng, Qi Wang, Xiudan Chen, Hao Wang, Weilin Zhang, Huan Wang, Lingling Song, Linsheng 2017-02-01 http://ir.qdio.ac.cn/handle/337002/136789 https://doi.org/10.1016/j.fsi.2016.12.005 英语 eng FISH & SHELLFISH IMMUNOLOGY Liu, Rui,Cheng, Qi,Wang, Xiudan,et al. The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2017,61:68-78. http://ir.qdio.ac.cn/handle/337002/136789 doi:10.1016/j.fsi.2016.12.005 B-cell Translocation Gene 1 Crassostrea Gigas Anti-proliferation Apoptosis Immune Response Pathogen Infection Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.fsi.2016.12.005 2022-06-27T05:38:01Z B-cell translocation gene 1 (BTG1) is a member of the anti-proliferative gene family, which plays important roles in regulation of cell cycle. In the present study, a B-cell translocation gene 1 molecule homologue (designed CgBTG1) are identified and characterized in oyster Crassostrea gigas. CgBTG1 contains a conserved BTG domain with Box A and Box B motifs, and it shares high similarities with both BTG1 and BTG2 proteins in vertebrates. CgBTG1 mRNA is predominantly expressed in hemocytes, and its expression level in hemocytes is significantly up-regulated at 6 h (5.40-fold, p < 0.01) post Vibrio splendidus stimulation. The apoptosis rate of oyster hemocytes is significantly decreased (p < 0.05) after CgBTG1 interfered by dsRNA (dsCgBTG1). This is indicated that CgBTG1 participated in the regulation of oyster hemocytes apoptosis. Furthermore, CgBTG1 could also induce the apoptosis of cancer cells (HeLa, A549 and BEL7402) in vitro. Compared with normal oysters, both vessel-like structures and muscle fibers in CgBTG1 interfered oysters are severely damaged after V. splendidus challenge in paraffin section, considering that CgBTG1 possessed an analogous feature of angiogenesis for maintenance of vessel-like structures in adductor muscle of oyster. The results suggests that CgBTG1 is a multi-functional molecule involved in the immune response of C. gigas against pathogen infection, which provides more clues for intensive studies of BTG family proteins in invertebrates. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 61 68 78
institution Open Polar
collection Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR
op_collection_id ftchinacasciocas
language English
topic B-cell Translocation Gene 1
Crassostrea Gigas
Anti-proliferation
Apoptosis
Immune Response
Pathogen Infection
spellingShingle B-cell Translocation Gene 1
Crassostrea Gigas
Anti-proliferation
Apoptosis
Immune Response
Pathogen Infection
Liu, Rui
Cheng, Qi
Wang, Xiudan
Chen, Hao
Wang, Weilin
Zhang, Huan
Wang, Lingling
Song, Linsheng
The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response
topic_facet B-cell Translocation Gene 1
Crassostrea Gigas
Anti-proliferation
Apoptosis
Immune Response
Pathogen Infection
description B-cell translocation gene 1 (BTG1) is a member of the anti-proliferative gene family, which plays important roles in regulation of cell cycle. In the present study, a B-cell translocation gene 1 molecule homologue (designed CgBTG1) are identified and characterized in oyster Crassostrea gigas. CgBTG1 contains a conserved BTG domain with Box A and Box B motifs, and it shares high similarities with both BTG1 and BTG2 proteins in vertebrates. CgBTG1 mRNA is predominantly expressed in hemocytes, and its expression level in hemocytes is significantly up-regulated at 6 h (5.40-fold, p < 0.01) post Vibrio splendidus stimulation. The apoptosis rate of oyster hemocytes is significantly decreased (p < 0.05) after CgBTG1 interfered by dsRNA (dsCgBTG1). This is indicated that CgBTG1 participated in the regulation of oyster hemocytes apoptosis. Furthermore, CgBTG1 could also induce the apoptosis of cancer cells (HeLa, A549 and BEL7402) in vitro. Compared with normal oysters, both vessel-like structures and muscle fibers in CgBTG1 interfered oysters are severely damaged after V. splendidus challenge in paraffin section, considering that CgBTG1 possessed an analogous feature of angiogenesis for maintenance of vessel-like structures in adductor muscle of oyster. The results suggests that CgBTG1 is a multi-functional molecule involved in the immune response of C. gigas against pathogen infection, which provides more clues for intensive studies of BTG family proteins in invertebrates. (C) 2016 Elsevier Ltd. All rights reserved.
format Article in Journal/Newspaper
author Liu, Rui
Cheng, Qi
Wang, Xiudan
Chen, Hao
Wang, Weilin
Zhang, Huan
Wang, Lingling
Song, Linsheng
author_facet Liu, Rui
Cheng, Qi
Wang, Xiudan
Chen, Hao
Wang, Weilin
Zhang, Huan
Wang, Lingling
Song, Linsheng
author_sort Liu, Rui
title The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response
title_short The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response
title_full The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response
title_fullStr The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response
title_full_unstemmed The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response
title_sort b-cell translocation gene 1 (cgbtg1) identified in oyster crassostrea gigas exhibit multiple functions in immune response
publishDate 2017
url http://ir.qdio.ac.cn/handle/337002/136789
https://doi.org/10.1016/j.fsi.2016.12.005
genre Crassostrea gigas
genre_facet Crassostrea gigas
op_relation FISH & SHELLFISH IMMUNOLOGY
Liu, Rui,Cheng, Qi,Wang, Xiudan,et al. The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2017,61:68-78.
http://ir.qdio.ac.cn/handle/337002/136789
doi:10.1016/j.fsi.2016.12.005
op_doi https://doi.org/10.1016/j.fsi.2016.12.005
container_title Fish & Shellfish Immunology
container_volume 61
container_start_page 68
op_container_end_page 78
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