The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus
Increasing evidences have demonstrated that the invertebrate gill is a predominant tissue participating in the immune response during pathogen challenge. In the present study, the hematopoiesis and immune activities in gill of Pacific oyster Crassostrea gigas were investigated. Stem-like cells with...
Published in: | Developmental & Comparative Immunology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/136728 https://doi.org/10.1016/j.dci.2017.01.024 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136728 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136728 2023-05-15T15:57:57+02:00 The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus Li, Yiqun Song, Xiaorui Wang, Weilin Wang, Lingling Yi, Qilin Jiang, Shuai Jia, Zhihao Du, Xinyu Qiu, Limei Song, Linsheng 2017-06-01 http://ir.qdio.ac.cn/handle/337002/136728 https://doi.org/10.1016/j.dci.2017.01.024 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Li, Yiqun,Song, Xiaorui,Wang, Weilin,et al. The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,71:59-69. http://ir.qdio.ac.cn/handle/337002/136728 doi:10.1016/j.dci.2017.01.024 Crassostrea Gigas Gill Hematopoiesis Immune Priming Cell Regeneration Enhanced Immune Response Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.dci.2017.01.024 2022-06-27T05:38:01Z Increasing evidences have demonstrated that the invertebrate gill is a predominant tissue participating in the immune response during pathogen challenge. In the present study, the hematopoiesis and immune activities in gill of Pacific oyster Crassostrea gigas were investigated. Stem-like cells with big nuclei and thin cytoplasm were found in the tubules of gill filaments, where DNA synthesis is active and hemocytes production are exuberant. The oysters primarily stimulated by formaldehyde-killed Vibrio splendidus exhibited stronger immune responses and enhanced cell regeneration in gill when they encountered the secondary challenge of live V. splendidus. After the secondary stimulation with V. splendidus, the expression levels of CgClec-4 and CgIFN in the gill of oysters pre-stimulated with formaldehyde-killed V. splendidus were significantly higher (p < 0.05) than that in the oysters pre-stimulated with filter-sterilized (0.22 mu m pore size) sea water, while the expression level of CgIL-17 was significantly decreased (p < 0.05). Meanwhile, the protein expression level of hematopoietic transcription factor CgGATA3 and immune-related protein CgEcSOD in gill increased apparently after the secondary challenge with V. splendidus. ROS production was also enhanced (p < 0.05) at 6 h and 24 h after the secondary challenge. The phagocytic rate in gill of oysters pre-stimulated with formaldehyde-killed V. splendidus was significantly increased (p < 0.05) at 6 h after the secondary challenge with live V. splendidus, showing faster response than that pre-stimulated with filter-sterilized sea water. These results collectively showed that the immune parameters in gill were apparently enhanced after secondary challenge With live V. splendidus, indicating that hematopoiesis might participate in immune priming in Pacific oyster C gigas. (C) 2017 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 71 59 69 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Gill Hematopoiesis Immune Priming Cell Regeneration Enhanced Immune Response |
spellingShingle |
Crassostrea Gigas Gill Hematopoiesis Immune Priming Cell Regeneration Enhanced Immune Response Li, Yiqun Song, Xiaorui Wang, Weilin Wang, Lingling Yi, Qilin Jiang, Shuai Jia, Zhihao Du, Xinyu Qiu, Limei Song, Linsheng The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus |
topic_facet |
Crassostrea Gigas Gill Hematopoiesis Immune Priming Cell Regeneration Enhanced Immune Response |
description |
Increasing evidences have demonstrated that the invertebrate gill is a predominant tissue participating in the immune response during pathogen challenge. In the present study, the hematopoiesis and immune activities in gill of Pacific oyster Crassostrea gigas were investigated. Stem-like cells with big nuclei and thin cytoplasm were found in the tubules of gill filaments, where DNA synthesis is active and hemocytes production are exuberant. The oysters primarily stimulated by formaldehyde-killed Vibrio splendidus exhibited stronger immune responses and enhanced cell regeneration in gill when they encountered the secondary challenge of live V. splendidus. After the secondary stimulation with V. splendidus, the expression levels of CgClec-4 and CgIFN in the gill of oysters pre-stimulated with formaldehyde-killed V. splendidus were significantly higher (p < 0.05) than that in the oysters pre-stimulated with filter-sterilized (0.22 mu m pore size) sea water, while the expression level of CgIL-17 was significantly decreased (p < 0.05). Meanwhile, the protein expression level of hematopoietic transcription factor CgGATA3 and immune-related protein CgEcSOD in gill increased apparently after the secondary challenge with V. splendidus. ROS production was also enhanced (p < 0.05) at 6 h and 24 h after the secondary challenge. The phagocytic rate in gill of oysters pre-stimulated with formaldehyde-killed V. splendidus was significantly increased (p < 0.05) at 6 h after the secondary challenge with live V. splendidus, showing faster response than that pre-stimulated with filter-sterilized sea water. These results collectively showed that the immune parameters in gill were apparently enhanced after secondary challenge With live V. splendidus, indicating that hematopoiesis might participate in immune priming in Pacific oyster C gigas. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Li, Yiqun Song, Xiaorui Wang, Weilin Wang, Lingling Yi, Qilin Jiang, Shuai Jia, Zhihao Du, Xinyu Qiu, Limei Song, Linsheng |
author_facet |
Li, Yiqun Song, Xiaorui Wang, Weilin Wang, Lingling Yi, Qilin Jiang, Shuai Jia, Zhihao Du, Xinyu Qiu, Limei Song, Linsheng |
author_sort |
Li, Yiqun |
title |
The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus |
title_short |
The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus |
title_full |
The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus |
title_fullStr |
The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus |
title_full_unstemmed |
The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus |
title_sort |
hematopoiesis in gill and its role in the immune response of pacific oyster crassostrea gigas against secondary challenge with vibrio splendidus |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/136728 https://doi.org/10.1016/j.dci.2017.01.024 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Li, Yiqun,Song, Xiaorui,Wang, Weilin,et al. The hematopoiesis in gill and its role in the immune response of Pacific oyster Crassostrea gigas against secondary challenge with Vibrio splendidus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,71:59-69. http://ir.qdio.ac.cn/handle/337002/136728 doi:10.1016/j.dci.2017.01.024 |
op_doi |
https://doi.org/10.1016/j.dci.2017.01.024 |
container_title |
Developmental & Comparative Immunology |
container_volume |
71 |
container_start_page |
59 |
op_container_end_page |
69 |
_version_ |
1766393653324939264 |