A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas
Molluscan development involves important features that are important to understanding not only molluscan ontogeny but also animal evolution. To gain insight into the gamete proteome and protein function in fertilization and early development, we analyzed the proteomes of unfertilized oocytes and ear...
Published in: | PROTEOMICS |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/136405 https://doi.org/10.1002/pmic.201600251 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136405 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136405 2023-05-15T15:57:40+02:00 A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas Huang, Xiaohong Huan, Pin Liu, Baozhong 2017 http://ir.qdio.ac.cn/handle/337002/136405 https://doi.org/10.1002/pmic.201600251 英语 eng PROTEOMICS Huang, Xiaohong,Huan, Pin,Liu, Baozhong. A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas[J]. PROTEOMICS,2017,17(1-2). http://ir.qdio.ac.cn/handle/337002/136405 doi:10.1002/pmic.201600251 2de Animal Proteomics Crassostrea Gigas Development Fertilization Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1002/pmic.201600251 2022-06-27T05:37:54Z Molluscan development involves important features that are important to understanding not only molluscan ontogeny but also animal evolution. To gain insight into the gamete proteome and protein function in fertilization and early development, we analyzed the proteomes of unfertilized oocytes and early embryos (2/4-cell stage) of the Pacific oyster, Crassostrea gigas. An oocyte reference map containing 116 protein spots, of which 69 were identified, revealed a high abundance of vitellogenin-derived protein spots. The differentially regulated protein spots during fertilization were screened using comparative proteomic approaches. In total, 18 differentially regulated protein spots were screened, and 15 of these were identified and divided into three groups. The proteins belonging to the first group function in energy supply and antioxidation and are proposed to ensure successful fertilization by regulating the levels of adenosine triphosphate, resisting oxidative stress, and preventing polyspermy. The proteins of the second group are associated with protein synthesis and modification, reflecting active protein synthesis after fertilization. The three proteins belonging to the final group are hypothesized to function in the regulation of embryonic development through the establishment of cell polarity and modulation of methylation reactions in nuclei. These results will enhance our knowledge of molluscan fertilization and development. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific PROTEOMICS 17 1-2 1600251 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
2de Animal Proteomics Crassostrea Gigas Development Fertilization |
spellingShingle |
2de Animal Proteomics Crassostrea Gigas Development Fertilization Huang, Xiaohong Huan, Pin Liu, Baozhong A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas |
topic_facet |
2de Animal Proteomics Crassostrea Gigas Development Fertilization |
description |
Molluscan development involves important features that are important to understanding not only molluscan ontogeny but also animal evolution. To gain insight into the gamete proteome and protein function in fertilization and early development, we analyzed the proteomes of unfertilized oocytes and early embryos (2/4-cell stage) of the Pacific oyster, Crassostrea gigas. An oocyte reference map containing 116 protein spots, of which 69 were identified, revealed a high abundance of vitellogenin-derived protein spots. The differentially regulated protein spots during fertilization were screened using comparative proteomic approaches. In total, 18 differentially regulated protein spots were screened, and 15 of these were identified and divided into three groups. The proteins belonging to the first group function in energy supply and antioxidation and are proposed to ensure successful fertilization by regulating the levels of adenosine triphosphate, resisting oxidative stress, and preventing polyspermy. The proteins of the second group are associated with protein synthesis and modification, reflecting active protein synthesis after fertilization. The three proteins belonging to the final group are hypothesized to function in the regulation of embryonic development through the establishment of cell polarity and modulation of methylation reactions in nuclei. These results will enhance our knowledge of molluscan fertilization and development. |
format |
Article in Journal/Newspaper |
author |
Huang, Xiaohong Huan, Pin Liu, Baozhong |
author_facet |
Huang, Xiaohong Huan, Pin Liu, Baozhong |
author_sort |
Huang, Xiaohong |
title |
A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas |
title_short |
A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas |
title_full |
A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas |
title_fullStr |
A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas |
title_full_unstemmed |
A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas |
title_sort |
comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster crassostrea gigas |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/136405 https://doi.org/10.1002/pmic.201600251 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
PROTEOMICS Huang, Xiaohong,Huan, Pin,Liu, Baozhong. A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas[J]. PROTEOMICS,2017,17(1-2). http://ir.qdio.ac.cn/handle/337002/136405 doi:10.1002/pmic.201600251 |
op_doi |
https://doi.org/10.1002/pmic.201600251 |
container_title |
PROTEOMICS |
container_volume |
17 |
container_issue |
1-2 |
container_start_page |
1600251 |
_version_ |
1766393336444223488 |