The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas
Hemocytes comprise diverse cell types with morphological and functional heterogeneity and play indispensable roles in immunological homeostasis of invertebrates. The morphological classification of different hemocytes in mollusk has been studied since the 1970's, yet the involvement of the diff...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/136251 https://doi.org/10.1016/j.dci.2016.09.017 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136251 2023-05-15T15:58:02+02:00 The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas Wang, Weilin Li, Meijia Wang, Lingling Chen, Hao Liu, Zhaoqun Jia, Zhihao Qiu, Limei Song, Linsheng 2017-02-01 http://ir.qdio.ac.cn/handle/337002/136251 https://doi.org/10.1016/j.dci.2016.09.017 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Wang, Weilin,Li, Meijia,Wang, Lingling,et al. The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:221-228. http://ir.qdio.ac.cn/handle/337002/136251 doi:10.1016/j.dci.2016.09.017 Crassostrea Gigas Hemocyte Separation Granulocytes Immunocompetent Functional Features Article 期刊论文 2017 ftchinacasciocas https://doi.org/10.1016/j.dci.2016.09.017 2022-06-27T05:37:51Z Hemocytes comprise diverse cell types with morphological and functional heterogeneity and play indispensable roles in immunological homeostasis of invertebrates. The morphological classification of different hemocytes in mollusk has been studied since the 1970's, yet the involvement of the different sub-populations in immune functions is far from clear. In the present study, three types of hemocytes were morphologically identified and separated as agranulocytes, semi-granulocytes and granulocytes by flow cytometry and Percoll density gradient centrifugation. The granulocytes were characterized functionally as the main phagocytic and encapsulating population, while semi-granulocytes and agranulocytes exhibited low or no such capacities, respectively. Meanwhile, the lysosome activity and the productions of ROS and NO were all mainly concentrated in granulocytes under both normal and immune-activated situations. Further, the mRNA transcripts of some immune related genes, including CgTLR, CgClathrin, CgATPeV, CgLysozyme, CgDefensin and CgIL-17, were mainly expressed in granulocytes, lower in semi-granulocytes and agranulocytes. These results collectively suggested that the granulocytes were the main immunocompetent hemocytes in oyster C. gigas, and a differentiation relationship among these three sub-population hemocytes was inferred based on the gradual changes in morphological, functional and molecular features. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 67 221 228 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Hemocyte Separation Granulocytes Immunocompetent Functional Features |
spellingShingle |
Crassostrea Gigas Hemocyte Separation Granulocytes Immunocompetent Functional Features Wang, Weilin Li, Meijia Wang, Lingling Chen, Hao Liu, Zhaoqun Jia, Zhihao Qiu, Limei Song, Linsheng The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas |
topic_facet |
Crassostrea Gigas Hemocyte Separation Granulocytes Immunocompetent Functional Features |
description |
Hemocytes comprise diverse cell types with morphological and functional heterogeneity and play indispensable roles in immunological homeostasis of invertebrates. The morphological classification of different hemocytes in mollusk has been studied since the 1970's, yet the involvement of the different sub-populations in immune functions is far from clear. In the present study, three types of hemocytes were morphologically identified and separated as agranulocytes, semi-granulocytes and granulocytes by flow cytometry and Percoll density gradient centrifugation. The granulocytes were characterized functionally as the main phagocytic and encapsulating population, while semi-granulocytes and agranulocytes exhibited low or no such capacities, respectively. Meanwhile, the lysosome activity and the productions of ROS and NO were all mainly concentrated in granulocytes under both normal and immune-activated situations. Further, the mRNA transcripts of some immune related genes, including CgTLR, CgClathrin, CgATPeV, CgLysozyme, CgDefensin and CgIL-17, were mainly expressed in granulocytes, lower in semi-granulocytes and agranulocytes. These results collectively suggested that the granulocytes were the main immunocompetent hemocytes in oyster C. gigas, and a differentiation relationship among these three sub-population hemocytes was inferred based on the gradual changes in morphological, functional and molecular features. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Wang, Weilin Li, Meijia Wang, Lingling Chen, Hao Liu, Zhaoqun Jia, Zhihao Qiu, Limei Song, Linsheng |
author_facet |
Wang, Weilin Li, Meijia Wang, Lingling Chen, Hao Liu, Zhaoqun Jia, Zhihao Qiu, Limei Song, Linsheng |
author_sort |
Wang, Weilin |
title |
The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas |
title_short |
The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas |
title_full |
The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas |
title_fullStr |
The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas |
title_full_unstemmed |
The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas |
title_sort |
granulocytes are the main immunocompetent hemocytes in crassostrea gigas |
publishDate |
2017 |
url |
http://ir.qdio.ac.cn/handle/337002/136251 https://doi.org/10.1016/j.dci.2016.09.017 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Wang, Weilin,Li, Meijia,Wang, Lingling,et al. The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:221-228. http://ir.qdio.ac.cn/handle/337002/136251 doi:10.1016/j.dci.2016.09.017 |
op_doi |
https://doi.org/10.1016/j.dci.2016.09.017 |
container_title |
Developmental & Comparative Immunology |
container_volume |
67 |
container_start_page |
221 |
op_container_end_page |
228 |
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1766393753203900416 |