Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR
Aeromonas salmonicida, an important pathogenic bacterium which induces furunculosis, is globally causing increased risks in Atlantic salmon (Salmo salar) farming. Although the kidney is the main target organ of A. salmonicida, the metabolic profiling of kidney in response to A. salmonicida in vivo r...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/136193 2023-05-15T15:29:57+02:00 Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying 2016-11-01 http://ir.qdio.ac.cn/handle/337002/136193 https://doi.org/10.1016/j.fsi.2016.08.055 英语 eng FISH & SHELLFISH IMMUNOLOGY Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:292-301. http://ir.qdio.ac.cn/handle/337002/136193 doi:10.1016/j.fsi.2016.08.055 Metabolomics Nmr H-1 Nmr Spectroscopy Aeromonas Salmonicida Atlantic Salmon Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.fsi.2016.08.055 2022-06-27T05:37:48Z Aeromonas salmonicida, an important pathogenic bacterium which induces furunculosis, is globally causing increased risks in Atlantic salmon (Salmo salar) farming. Although the kidney is the main target organ of A. salmonicida, the metabolic profiling of kidney in response to A. salmonicida in vivo remains unknown. Here, we used H-1 nuclear magnetic resonance (NMR) to comprehensively analyze the metabolic changes in the kidney of Atlantic salmon. Through the NOESYPRID spectrum combined with multi-variate pattern recognition analysis, including principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) models, significant metabolic changes were observed seven and 14 days post-infection and in a control group. Hence, the main objective of this study was to estimate the significant metabolites with resistance to furunculosis and further understand the mechanism of A. salmonicida in Atlantic salmon. Notably, substantial alterations of kidney metabolites were observed, such as with fumarate, alanine, valine, glycine, aspartate, choline, glycerophosphocholine and betaine; and summarized by metabolic pathways including the citrate cycle, glycolysis/gluconeo-genesis, tryptophan metabolism, and urea cycle, respectively. Changes were also observed in 3-hydroxybutyrate and phosphocholine which were not involved in these four metabolic pathways. After analyzing the alteration trend of these metabolites, we inferred that A. salmonicida caused absorption inhibition of amino acids and disturbed protein metabolism as well as cell metabolism in favor of its replication. These observations offered novel insights into the mechanisms of infection at a functional level and facilitated further assessment and clarification of fish disease from A. salmonicida exposure. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Atlantic salmon Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 58 292 301 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Metabolomics Nmr H-1 Nmr Spectroscopy Aeromonas Salmonicida Atlantic Salmon |
spellingShingle |
Metabolomics Nmr H-1 Nmr Spectroscopy Aeromonas Salmonicida Atlantic Salmon Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR |
topic_facet |
Metabolomics Nmr H-1 Nmr Spectroscopy Aeromonas Salmonicida Atlantic Salmon |
description |
Aeromonas salmonicida, an important pathogenic bacterium which induces furunculosis, is globally causing increased risks in Atlantic salmon (Salmo salar) farming. Although the kidney is the main target organ of A. salmonicida, the metabolic profiling of kidney in response to A. salmonicida in vivo remains unknown. Here, we used H-1 nuclear magnetic resonance (NMR) to comprehensively analyze the metabolic changes in the kidney of Atlantic salmon. Through the NOESYPRID spectrum combined with multi-variate pattern recognition analysis, including principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) models, significant metabolic changes were observed seven and 14 days post-infection and in a control group. Hence, the main objective of this study was to estimate the significant metabolites with resistance to furunculosis and further understand the mechanism of A. salmonicida in Atlantic salmon. Notably, substantial alterations of kidney metabolites were observed, such as with fumarate, alanine, valine, glycine, aspartate, choline, glycerophosphocholine and betaine; and summarized by metabolic pathways including the citrate cycle, glycolysis/gluconeo-genesis, tryptophan metabolism, and urea cycle, respectively. Changes were also observed in 3-hydroxybutyrate and phosphocholine which were not involved in these four metabolic pathways. After analyzing the alteration trend of these metabolites, we inferred that A. salmonicida caused absorption inhibition of amino acids and disturbed protein metabolism as well as cell metabolism in favor of its replication. These observations offered novel insights into the mechanisms of infection at a functional level and facilitated further assessment and clarification of fish disease from A. salmonicida exposure. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying |
author_facet |
Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Liu, Ying |
author_sort |
Liu, Peng-fei |
title |
Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR |
title_short |
Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR |
title_full |
Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR |
title_fullStr |
Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR |
title_full_unstemmed |
Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR |
title_sort |
metabolic profiling in kidneys of atlantic salmon infected with aeromonas salmonicida based on h-1 nmr |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/136193 https://doi.org/10.1016/j.fsi.2016.08.055 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:292-301. http://ir.qdio.ac.cn/handle/337002/136193 doi:10.1016/j.fsi.2016.08.055 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.08.055 |
container_title |
Fish & Shellfish Immunology |
container_volume |
58 |
container_start_page |
292 |
op_container_end_page |
301 |
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1766360407538139136 |