A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway
Interferon is a highly pleiotropic cytokine, once binding to its receptors, can activate JAK kinases and STAT transcription factors to initiate the transcription of genes downstream from interferon-stimulated response elements. In the present study, a cytokine receptor-like 3 molecule was selected a...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/135895 2023-05-15T15:57:42+02:00 A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway Zhang, Ran Liu, Rui Xin, Lusheng Chen, Hao Li, Chenghua Wang, Lingling Song, Linsheng 2016-12-01 http://ir.qdio.ac.cn/handle/337002/135895 https://doi.org/10.1016/j.dci.2016.06.010 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Zhang, Ran,Liu, Rui,Xin, Lusheng,et al. A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:98-106. http://ir.qdio.ac.cn/handle/337002/135895 doi:10.1016/j.dci.2016.06.010 Crassostrea Gigas Interferon Interferon Receptor Poly (i: c) Interferon Stimulated Genes Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.dci.2016.06.010 2022-06-27T05:37:45Z Interferon is a highly pleiotropic cytokine, once binding to its receptors, can activate JAK kinases and STAT transcription factors to initiate the transcription of genes downstream from interferon-stimulated response elements. In the present study, a cytokine receptor-like 3 molecule was selected and cloned from oyster Crassostrea gigas, which contained a fibronectin type III domain (designed CgIFNR-3). The expression pattern of CgIFNR-3 mRNA was detected in all the tested tissues including mantle, gills, hepatopancreas, muscle, and hemocytes, with the highest expression level in hemocytes. After poly (I: C) stimulation, the expression level of CgIFNR-3 in hemocytes was observed to significantly increase at 3 h (13.06-fold, p < 0.01). CgIFNR-3 was indicated to interact with CgIFNLP by in vitro GST pull-down assay, and to activate the expression of transcription factors including ISRE, STAT3 and GAS, in human Janus kinase signal transducer and activator of transcription (JAK/STAT) pathway after co-transfection in HEK-293T cells in the reporter luciferase activity assay. These results suggested that CgIFNR-3 could bind to CgIFNLP as an interferon receptor and participate in the activation of JAK/STAT pathway in human, which will benefit for intensive studies of interferon signaling pathway in mollusc. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Janus ENVELOPE(163.100,163.100,-71.067,-71.067) Developmental & Comparative Immunology 65 98 106 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Interferon Interferon Receptor Poly (i: c) Interferon Stimulated Genes |
spellingShingle |
Crassostrea Gigas Interferon Interferon Receptor Poly (i: c) Interferon Stimulated Genes Zhang, Ran Liu, Rui Xin, Lusheng Chen, Hao Li, Chenghua Wang, Lingling Song, Linsheng A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway |
topic_facet |
Crassostrea Gigas Interferon Interferon Receptor Poly (i: c) Interferon Stimulated Genes |
description |
Interferon is a highly pleiotropic cytokine, once binding to its receptors, can activate JAK kinases and STAT transcription factors to initiate the transcription of genes downstream from interferon-stimulated response elements. In the present study, a cytokine receptor-like 3 molecule was selected and cloned from oyster Crassostrea gigas, which contained a fibronectin type III domain (designed CgIFNR-3). The expression pattern of CgIFNR-3 mRNA was detected in all the tested tissues including mantle, gills, hepatopancreas, muscle, and hemocytes, with the highest expression level in hemocytes. After poly (I: C) stimulation, the expression level of CgIFNR-3 in hemocytes was observed to significantly increase at 3 h (13.06-fold, p < 0.01). CgIFNR-3 was indicated to interact with CgIFNLP by in vitro GST pull-down assay, and to activate the expression of transcription factors including ISRE, STAT3 and GAS, in human Janus kinase signal transducer and activator of transcription (JAK/STAT) pathway after co-transfection in HEK-293T cells in the reporter luciferase activity assay. These results suggested that CgIFNR-3 could bind to CgIFNLP as an interferon receptor and participate in the activation of JAK/STAT pathway in human, which will benefit for intensive studies of interferon signaling pathway in mollusc. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Zhang, Ran Liu, Rui Xin, Lusheng Chen, Hao Li, Chenghua Wang, Lingling Song, Linsheng |
author_facet |
Zhang, Ran Liu, Rui Xin, Lusheng Chen, Hao Li, Chenghua Wang, Lingling Song, Linsheng |
author_sort |
Zhang, Ran |
title |
A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway |
title_short |
A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway |
title_full |
A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway |
title_fullStr |
A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway |
title_full_unstemmed |
A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway |
title_sort |
cgifnlp receptor from crassostrea gigas and its activation of the related genes in human jak/stat signaling pathway |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/135895 https://doi.org/10.1016/j.dci.2016.06.010 |
long_lat |
ENVELOPE(163.100,163.100,-71.067,-71.067) |
geographic |
Janus |
geographic_facet |
Janus |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Zhang, Ran,Liu, Rui,Xin, Lusheng,et al. A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:98-106. http://ir.qdio.ac.cn/handle/337002/135895 doi:10.1016/j.dci.2016.06.010 |
op_doi |
https://doi.org/10.1016/j.dci.2016.06.010 |
container_title |
Developmental & Comparative Immunology |
container_volume |
65 |
container_start_page |
98 |
op_container_end_page |
106 |
_version_ |
1766393373209395200 |