The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas
Phagocytes have been proved to play vital roles in the innate immune response. However, the cellular characteristics of phagocytes in invertebrates, especially in molluscs, remain largely unknown. In the present study, fluorescence activated cell sorting (FRCS) was employed to sort the phagocytes fr...
Published in: | Fish & Shellfish Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/135879 https://doi.org/10.1016/j.fsi.2016.06.024 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/135879 2023-05-15T15:57:50+02:00 The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas Jiang, Shuai Jia, Zhihao Xin, Lusheng Sun, Ying Zhang, Ran Wang, Weilin Wang, Lingling Song, Linsheng 2016-08-01 http://ir.qdio.ac.cn/handle/337002/135879 https://doi.org/10.1016/j.fsi.2016.06.024 英语 eng FISH & SHELLFISH IMMUNOLOGY Jiang, Shuai,Jia, Zhihao,Xin, Lusheng,et al. The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:490-498. http://ir.qdio.ac.cn/handle/337002/135879 doi:10.1016/j.fsi.2016.06.024 Crassostrea Gigas Phagocyte Cytochemical Staining Ultrastructural Analysis Cytokine Lysosome Reactive Oxygen Species Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.fsi.2016.06.024 2022-06-27T05:37:45Z Phagocytes have been proved to play vital roles in the innate immune response. However, the cellular characteristics of phagocytes in invertebrates, especially in molluscs, remain largely unknown. In the present study, fluorescence activated cell sorting (FRCS) was employed to sort the phagocytes from the non-phagocytic haemocytes of the Pacific oyster Crassostrea gigas. The cytochemical staining analysis revealed that phagocytes were positive staining for alpha-naphthyl acetate esterase and myeloperoxidase, while negative staining for toluidine blue and periodic acid-Schiff. The non-phagocytic haemocytes exhibited positive staining for periodic acid-Schiff, weak positive staining for toluidine blue, but negative staining for alpha-naphthyl acetate esterase and myeloperoxidase. In addition, phagocytes exhibited ultra structural cellular features similar to those of macrophages, with large cell diameter, rough cell membrane and extended pseudopodia revealed by the scanning electron microscopy, while the non-phagocytic haemocytes exhibited small cell diameter, smooth cell surface and round spherical shape. Transmission electron microscopy further demonstrated that phagocytes were abundant of cytoplasmic bodies and mitochondria, while non-phagocytic haemocytes were characterized as the comparatively large cell nucleus with contorted and condensed heterochromatin adherent to the nuclear envelope. Moreover, compared with non-phagocytic haemocytes, phagocytes exhibited significantly higher levels of intracellular cytokines, including tumor necrosis factor, interferon-like protein and interleukin-17, and significantly higher abundance of lysosome and reactive oxygen species, which were of great importance to the activation of immune response and pathogen clearance. Taken together, these findings revealed the different cytochemical and ultrastructural features between phagocytes and non-phagocytic haemocytes in C. gigas, which would provide an important clue to investigate the mechanism of phagocytosis underlying the ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Fish & Shellfish Immunology 55 490 498 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Phagocyte Cytochemical Staining Ultrastructural Analysis Cytokine Lysosome Reactive Oxygen Species |
spellingShingle |
Crassostrea Gigas Phagocyte Cytochemical Staining Ultrastructural Analysis Cytokine Lysosome Reactive Oxygen Species Jiang, Shuai Jia, Zhihao Xin, Lusheng Sun, Ying Zhang, Ran Wang, Weilin Wang, Lingling Song, Linsheng The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea Gigas Phagocyte Cytochemical Staining Ultrastructural Analysis Cytokine Lysosome Reactive Oxygen Species |
description |
Phagocytes have been proved to play vital roles in the innate immune response. However, the cellular characteristics of phagocytes in invertebrates, especially in molluscs, remain largely unknown. In the present study, fluorescence activated cell sorting (FRCS) was employed to sort the phagocytes from the non-phagocytic haemocytes of the Pacific oyster Crassostrea gigas. The cytochemical staining analysis revealed that phagocytes were positive staining for alpha-naphthyl acetate esterase and myeloperoxidase, while negative staining for toluidine blue and periodic acid-Schiff. The non-phagocytic haemocytes exhibited positive staining for periodic acid-Schiff, weak positive staining for toluidine blue, but negative staining for alpha-naphthyl acetate esterase and myeloperoxidase. In addition, phagocytes exhibited ultra structural cellular features similar to those of macrophages, with large cell diameter, rough cell membrane and extended pseudopodia revealed by the scanning electron microscopy, while the non-phagocytic haemocytes exhibited small cell diameter, smooth cell surface and round spherical shape. Transmission electron microscopy further demonstrated that phagocytes were abundant of cytoplasmic bodies and mitochondria, while non-phagocytic haemocytes were characterized as the comparatively large cell nucleus with contorted and condensed heterochromatin adherent to the nuclear envelope. Moreover, compared with non-phagocytic haemocytes, phagocytes exhibited significantly higher levels of intracellular cytokines, including tumor necrosis factor, interferon-like protein and interleukin-17, and significantly higher abundance of lysosome and reactive oxygen species, which were of great importance to the activation of immune response and pathogen clearance. Taken together, these findings revealed the different cytochemical and ultrastructural features between phagocytes and non-phagocytic haemocytes in C. gigas, which would provide an important clue to investigate the mechanism of phagocytosis underlying the ... |
format |
Article in Journal/Newspaper |
author |
Jiang, Shuai Jia, Zhihao Xin, Lusheng Sun, Ying Zhang, Ran Wang, Weilin Wang, Lingling Song, Linsheng |
author_facet |
Jiang, Shuai Jia, Zhihao Xin, Lusheng Sun, Ying Zhang, Ran Wang, Weilin Wang, Lingling Song, Linsheng |
author_sort |
Jiang, Shuai |
title |
The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas |
title_short |
The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas |
title_full |
The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas |
title_fullStr |
The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas |
title_sort |
cytochemical and ultrastructural characteristics of phagocytes in the pacific oyster crassostrea gigas |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/135879 https://doi.org/10.1016/j.fsi.2016.06.024 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Jiang, Shuai,Jia, Zhihao,Xin, Lusheng,et al. The cytochemical and ultrastructural characteristics of phagocytes in the Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:490-498. http://ir.qdio.ac.cn/handle/337002/135879 doi:10.1016/j.fsi.2016.06.024 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.06.024 |
container_title |
Fish & Shellfish Immunology |
container_volume |
55 |
container_start_page |
490 |
op_container_end_page |
498 |
_version_ |
1766393528817025024 |