TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses
As a benthic filter feeder of estuaries, the immune system of oysters provides one of the best models for studying the genetic and molecular basis of the innate immune pathway in marine invertebrates and examining the influence of environmental factors on the immune system. Here, the molecular funct...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/135836 https://doi.org/10.1016/j.fsi.2016.07.011 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/135836 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/135836 2023-05-15T15:58:10+02:00 TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan 2016-09-01 http://ir.qdio.ac.cn/handle/337002/135836 https://doi.org/10.1016/j.fsi.2016.07.011 英语 eng FISH & SHELLFISH IMMUNOLOGY Tang, Xueying,Huang, Baoyu,Zhang, Linlin,et al. TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:330-335. http://ir.qdio.ac.cn/handle/337002/135836 doi:10.1016/j.fsi.2016.07.011 Pacific Oyster Crassostrea Gigas Tbk1 Innate Immunity Sting Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.fsi.2016.07.011 2022-06-27T05:37:42Z As a benthic filter feeder of estuaries, the immune system of oysters provides one of the best models for studying the genetic and molecular basis of the innate immune pathway in marine invertebrates and examining the influence of environmental factors on the immune system. Here, the molecular function of molluscan TANK-binding kinase-1 (TBK1) (which we named CgTBK1) was studied in the Pacific oyster, Crassostrea gigas. Compared with known TBK1 proteins in other model organisms, CgTBK1 contains a conserved S-TKc domain and a coiled coil domain at the N- and C-terminals but lacks an important ubiquitin domain. Quantitative real-time PCR analysis revealed that the expression level of CgTBK1 was ubiquitous in all selected tissues, with highest expression in the gills. CgTBK1 expression was significantly upregulated in response to infections with Vibrio alginolyticus, ostreid herpesvirus 1 (OsHV-1 reference strain and par), and polyinosinic:polycytidylic acid sodium salt, suggesting its broad function in immune response. Subcellular localization showed the presence of CgTBK1 in the cytoplasm of HeLa cells, suggesting its potential function as the signal transducer between the receptor and transcription factor. We further demonstrated that CgTBK1 interacted with CgSTING in HEK293T cells, providing evidence that CgTBK1 could be activated by direct binding to CgSTING. In summary, we characterized the TBK1 gene in C gigas and demonstrated its role in the innate immune response to pathogen infections. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Fish & Shellfish Immunology 56 330 335 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pacific Oyster Crassostrea Gigas Tbk1 Innate Immunity Sting |
spellingShingle |
Pacific Oyster Crassostrea Gigas Tbk1 Innate Immunity Sting Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses |
topic_facet |
Pacific Oyster Crassostrea Gigas Tbk1 Innate Immunity Sting |
description |
As a benthic filter feeder of estuaries, the immune system of oysters provides one of the best models for studying the genetic and molecular basis of the innate immune pathway in marine invertebrates and examining the influence of environmental factors on the immune system. Here, the molecular function of molluscan TANK-binding kinase-1 (TBK1) (which we named CgTBK1) was studied in the Pacific oyster, Crassostrea gigas. Compared with known TBK1 proteins in other model organisms, CgTBK1 contains a conserved S-TKc domain and a coiled coil domain at the N- and C-terminals but lacks an important ubiquitin domain. Quantitative real-time PCR analysis revealed that the expression level of CgTBK1 was ubiquitous in all selected tissues, with highest expression in the gills. CgTBK1 expression was significantly upregulated in response to infections with Vibrio alginolyticus, ostreid herpesvirus 1 (OsHV-1 reference strain and par), and polyinosinic:polycytidylic acid sodium salt, suggesting its broad function in immune response. Subcellular localization showed the presence of CgTBK1 in the cytoplasm of HeLa cells, suggesting its potential function as the signal transducer between the receptor and transcription factor. We further demonstrated that CgTBK1 interacted with CgSTING in HEK293T cells, providing evidence that CgTBK1 could be activated by direct binding to CgSTING. In summary, we characterized the TBK1 gene in C gigas and demonstrated its role in the innate immune response to pathogen infections. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan |
author_facet |
Tang, Xueying Huang, Baoyu Zhang, Linlin Li, Li Zhang, Guofan |
author_sort |
Tang, Xueying |
title |
TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses |
title_short |
TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses |
title_full |
TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses |
title_fullStr |
TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses |
title_full_unstemmed |
TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses |
title_sort |
tank-binding kinase-1 broadly affects oyster immune response to bacteria and viruses |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/135836 https://doi.org/10.1016/j.fsi.2016.07.011 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Tang, Xueying,Huang, Baoyu,Zhang, Linlin,et al. TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:330-335. http://ir.qdio.ac.cn/handle/337002/135836 doi:10.1016/j.fsi.2016.07.011 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.07.011 |
container_title |
Fish & Shellfish Immunology |
container_volume |
56 |
container_start_page |
330 |
op_container_end_page |
335 |
_version_ |
1766393899905974272 |