Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity
Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens iso...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/135725 2023-05-15T18:41:14+02:00 Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity Sun, Yuan-Yuan Chi, Heng Sun, Li 2016-08-23 http://ir.qdio.ac.cn/handle/337002/135725 https://doi.org/10.3389/fmicb.2010.01320 英语 eng FRONTIERS IN MICROBIOLOGY Sun, Yuan-Yuan,Chi, Heng,Sun, Li. Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity[J]. FRONTIERS IN MICROBIOLOGY,2016,7. http://ir.qdio.ac.cn/handle/337002/135725 doi:10.3389/fmicb.2010.01320 Pseudomonas Fluorescens Filamentous Hemagglutinin Motility Adhesion Virulence Vaccine Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.3389/fmicb.2010.01320 2022-06-27T05:37:42Z Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens isolate. In vitro study showed that compared to the wild type, the fha mutant TSSfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth and producing extracellular matrix, (ii) displayed no apparent flagella and motility, (iii) was defective in the attachment to host cells and unable to form self-aggregation, (iv) displayed markedly reduced capacity of hemagglutination and surviving in host serum. In vivo infection analysis revealed that TSSfha was significantly attenuated in the ability of dissemination in fish tissues and inducing host mortality, and that antibody blocking of the natural FHA produced by the wild type TSS impaired the infectivity of the pathogen. Furthermore, when introduced into turbot as a subunit vaccine, recombinant FHA elicited a significant protection against lethal TSS challenge. Taken together, these results indicate for the first time that P fluorescens FHA is a key virulence factor essential to multiple biological processes associated with pathogenicity. Article in Journal/Newspaper Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Pseudomonas Fluorescens Filamentous Hemagglutinin Motility Adhesion Virulence Vaccine |
spellingShingle |
Pseudomonas Fluorescens Filamentous Hemagglutinin Motility Adhesion Virulence Vaccine Sun, Yuan-Yuan Chi, Heng Sun, Li Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity |
topic_facet |
Pseudomonas Fluorescens Filamentous Hemagglutinin Motility Adhesion Virulence Vaccine |
description |
Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens isolate. In vitro study showed that compared to the wild type, the fha mutant TSSfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth and producing extracellular matrix, (ii) displayed no apparent flagella and motility, (iii) was defective in the attachment to host cells and unable to form self-aggregation, (iv) displayed markedly reduced capacity of hemagglutination and surviving in host serum. In vivo infection analysis revealed that TSSfha was significantly attenuated in the ability of dissemination in fish tissues and inducing host mortality, and that antibody blocking of the natural FHA produced by the wild type TSS impaired the infectivity of the pathogen. Furthermore, when introduced into turbot as a subunit vaccine, recombinant FHA elicited a significant protection against lethal TSS challenge. Taken together, these results indicate for the first time that P fluorescens FHA is a key virulence factor essential to multiple biological processes associated with pathogenicity. |
format |
Article in Journal/Newspaper |
author |
Sun, Yuan-Yuan Chi, Heng Sun, Li |
author_facet |
Sun, Yuan-Yuan Chi, Heng Sun, Li |
author_sort |
Sun, Yuan-Yuan |
title |
Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity |
title_short |
Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity |
title_full |
Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity |
title_fullStr |
Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity |
title_full_unstemmed |
Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity |
title_sort |
pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/135725 https://doi.org/10.3389/fmicb.2010.01320 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
FRONTIERS IN MICROBIOLOGY Sun, Yuan-Yuan,Chi, Heng,Sun, Li. Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity[J]. FRONTIERS IN MICROBIOLOGY,2016,7. http://ir.qdio.ac.cn/handle/337002/135725 doi:10.3389/fmicb.2010.01320 |
op_doi |
https://doi.org/10.3389/fmicb.2010.01320 |
_version_ |
1766230729847472128 |