Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus
To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot (Scophthalmus maximus), water temperature was lowered rapidly from 18 to 1 degrees C. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and...
Published in: | Chinese Journal of Oceanology and Limnology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/131069 2023-05-15T18:15:44+02:00 Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus Ji Liqin Jiang Keyong Liu Mei Wang Baojie Han Longjiang Zhang Mingming Wang Lei 2016-05-01 http://ir.qdio.ac.cn/handle/337002/131069 https://doi.org/10.1007/s00343-016-4367-z 英语 eng CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY Ji Liqin,Jiang Keyong,Liu Mei,et al. Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(3):430-440. http://ir.qdio.ac.cn/handle/337002/131069 doi:10.1007/s00343-016-4367-z Hematology Hsp70 Hsp90 Mrna Expression Low Temperature Stress Fish Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1007/s00343-016-4367-z 2022-06-27T05:37:42Z To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot (Scophthalmus maximus), water temperature was lowered rapidly from 18 to 1 degrees C. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1 degrees C. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly (P < 0.05) as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and gamma-glutamyl transpeptidase activities displayed no obvious changes above 1 degrees C and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8 degrees C, and creatinine concentration was highest at 3 degrees C. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly (P < 0.05) as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5 degrees C may induce a stress response in S. maximus and that temperature should be kept above 8 degrees C in the aquaculture setting to avoid damage to the fish. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Chinese Journal of Oceanology and Limnology 34 3 430 440 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Hematology Hsp70 Hsp90 Mrna Expression Low Temperature Stress Fish |
spellingShingle |
Hematology Hsp70 Hsp90 Mrna Expression Low Temperature Stress Fish Ji Liqin Jiang Keyong Liu Mei Wang Baojie Han Longjiang Zhang Mingming Wang Lei Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus |
topic_facet |
Hematology Hsp70 Hsp90 Mrna Expression Low Temperature Stress Fish |
description |
To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot (Scophthalmus maximus), water temperature was lowered rapidly from 18 to 1 degrees C. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1 degrees C. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly (P < 0.05) as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and gamma-glutamyl transpeptidase activities displayed no obvious changes above 1 degrees C and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8 degrees C, and creatinine concentration was highest at 3 degrees C. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly (P < 0.05) as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5 degrees C may induce a stress response in S. maximus and that temperature should be kept above 8 degrees C in the aquaculture setting to avoid damage to the fish. |
format |
Article in Journal/Newspaper |
author |
Ji Liqin Jiang Keyong Liu Mei Wang Baojie Han Longjiang Zhang Mingming Wang Lei |
author_facet |
Ji Liqin Jiang Keyong Liu Mei Wang Baojie Han Longjiang Zhang Mingming Wang Lei |
author_sort |
Ji Liqin |
title |
Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus |
title_short |
Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus |
title_full |
Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus |
title_fullStr |
Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus |
title_full_unstemmed |
Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus |
title_sort |
low temperature stress on the hematological parameters and hsp gene expression in the turbot scophthalmus maximus |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/131069 https://doi.org/10.1007/s00343-016-4367-z |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY Ji Liqin,Jiang Keyong,Liu Mei,et al. Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(3):430-440. http://ir.qdio.ac.cn/handle/337002/131069 doi:10.1007/s00343-016-4367-z |
op_doi |
https://doi.org/10.1007/s00343-016-4367-z |
container_title |
Chinese Journal of Oceanology and Limnology |
container_volume |
34 |
container_issue |
3 |
container_start_page |
430 |
op_container_end_page |
440 |
_version_ |
1766188943279128576 |