The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas
MyD88 serves as a critical cytosolic adaptor mediating activation of NF-kappa B in innate immunity. It has been found that there is a considerable expansion of MyD88 in Crassostrea gigas. In the present study, four typical MyD88 genes in Crassostrea gigas (CgMyD88-A to CgMyD88-D) were successfully c...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/130995 2023-05-15T15:57:41+02:00 The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas Xin, Lusheng Wang, Mengqiang Zhang, Huan Li, Meijia Wang, Hao Wang, Lingling Song, Linsheng 2016-07-01 http://ir.qdio.ac.cn/handle/337002/130995 https://doi.org/10.1016/j.fsi.2016.04.014 英语 eng FISH & SHELLFISH IMMUNOLOGY Xin, Lusheng,Wang, Mengqiang,Zhang, Huan,et al. The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:118-127. http://ir.qdio.ac.cn/handle/337002/130995 doi:10.1016/j.fsi.2016.04.014 Crassostrea Gigas Myd88 Innate Immunity Death Domain Tir Domain Nf-kappa b Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.fsi.2016.04.014 2022-06-27T05:37:39Z MyD88 serves as a critical cytosolic adaptor mediating activation of NF-kappa B in innate immunity. It has been found that there is a considerable expansion of MyD88 in Crassostrea gigas. In the present study, four typical MyD88 genes in Crassostrea gigas (CgMyD88-A to CgMyD88-D) were successfully cloned and their potential functions were investigated together with another two known ones (CgMyD88-T1 and CgMyD88-T2). Multiple alignments revealed that CgMyD88-B and CgMyD88-C remained the conserved DD and TIR domains, while there was a significant variation of E51Q in the DD of CgMyD88-A, and some variations in both DD and TIR domains of CgMyD88-D, respectively. Both truncated CgMyD88-T1 and CgMyD88-T2 lacked Box II in their only TIR domains. Expression pattern analysis showed that CgMyD88-B and CgMyD88-C genes possessed higher expression in normal tissues, compared with the other four. When oysters were under bacteria challenge, CgMyD88-B, CgMyD88-C, CgMyD88-T1 and CgMyD88-T2 were firstly induced, while CgMyD88-A and CgMyD88-D were suppressed. Dual luciferase reporter assays showed that CgMyD88-B and CgMyD88-C could promote the activation of NF-kappa B signaling pathway, while the other four CgMyD88 genes failed or even suppressed the activities of CgMyD88-B and CgMyD88-C on the activation of NF-kappa B signaling. It was deduced that after oysters were challenged by bacteria, CgMyD88-B and CgMyD88-C could rapidly and efficiently activate NF-kappa B signaling pathway to elicit anti-pathogen responses before suppressor CgMyD88 genes (CgMyD88-T1 and CgMyD88-T2) exceeding their expression level. These results suggested that there was mutual modulation of expanded CgMyD88 genes on activating NE-kappa B signaling pathway in oyster C. gigas. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 54 118 127 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Myd88 Innate Immunity Death Domain Tir Domain Nf-kappa b |
spellingShingle |
Crassostrea Gigas Myd88 Innate Immunity Death Domain Tir Domain Nf-kappa b Xin, Lusheng Wang, Mengqiang Zhang, Huan Li, Meijia Wang, Hao Wang, Lingling Song, Linsheng The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas |
topic_facet |
Crassostrea Gigas Myd88 Innate Immunity Death Domain Tir Domain Nf-kappa b |
description |
MyD88 serves as a critical cytosolic adaptor mediating activation of NF-kappa B in innate immunity. It has been found that there is a considerable expansion of MyD88 in Crassostrea gigas. In the present study, four typical MyD88 genes in Crassostrea gigas (CgMyD88-A to CgMyD88-D) were successfully cloned and their potential functions were investigated together with another two known ones (CgMyD88-T1 and CgMyD88-T2). Multiple alignments revealed that CgMyD88-B and CgMyD88-C remained the conserved DD and TIR domains, while there was a significant variation of E51Q in the DD of CgMyD88-A, and some variations in both DD and TIR domains of CgMyD88-D, respectively. Both truncated CgMyD88-T1 and CgMyD88-T2 lacked Box II in their only TIR domains. Expression pattern analysis showed that CgMyD88-B and CgMyD88-C genes possessed higher expression in normal tissues, compared with the other four. When oysters were under bacteria challenge, CgMyD88-B, CgMyD88-C, CgMyD88-T1 and CgMyD88-T2 were firstly induced, while CgMyD88-A and CgMyD88-D were suppressed. Dual luciferase reporter assays showed that CgMyD88-B and CgMyD88-C could promote the activation of NF-kappa B signaling pathway, while the other four CgMyD88 genes failed or even suppressed the activities of CgMyD88-B and CgMyD88-C on the activation of NF-kappa B signaling. It was deduced that after oysters were challenged by bacteria, CgMyD88-B and CgMyD88-C could rapidly and efficiently activate NF-kappa B signaling pathway to elicit anti-pathogen responses before suppressor CgMyD88 genes (CgMyD88-T1 and CgMyD88-T2) exceeding their expression level. These results suggested that there was mutual modulation of expanded CgMyD88 genes on activating NE-kappa B signaling pathway in oyster C. gigas. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Xin, Lusheng Wang, Mengqiang Zhang, Huan Li, Meijia Wang, Hao Wang, Lingling Song, Linsheng |
author_facet |
Xin, Lusheng Wang, Mengqiang Zhang, Huan Li, Meijia Wang, Hao Wang, Lingling Song, Linsheng |
author_sort |
Xin, Lusheng |
title |
The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas |
title_short |
The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas |
title_full |
The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas |
title_fullStr |
The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas |
title_full_unstemmed |
The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas |
title_sort |
categorization and mutual modulation of expanded myd88s in crassostrea gigas |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/130995 https://doi.org/10.1016/j.fsi.2016.04.014 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Xin, Lusheng,Wang, Mengqiang,Zhang, Huan,et al. The categorization and mutual modulation of expanded MyD88s in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:118-127. http://ir.qdio.ac.cn/handle/337002/130995 doi:10.1016/j.fsi.2016.04.014 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.04.014 |
container_title |
Fish & Shellfish Immunology |
container_volume |
54 |
container_start_page |
118 |
op_container_end_page |
127 |
_version_ |
1766393355358437376 |