Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis
The Antarctic green alga Chlamydomonas sp. ICE-L was isolated from sea ice. As a psychrophilic microalga, it can tolerate the environmental stress in the sea-ice brine, such as freezing temperature and high salinity. We performed a transcriptome analysis to identify freezing stress responding genes...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/130964 2023-05-15T13:40:37+02:00 Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis Liu, Chenlin Wang, Xiuliang Wang, Xingna Sun, Chengjun 2016-07-01 http://ir.qdio.ac.cn/handle/337002/130964 https://doi.org/10.1007/s00792-016-0834-x 英语 eng EXTREMOPHILES Liu, Chenlin,Wang, Xiuliang,Wang, Xingna,et al. Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis[J]. EXTREMOPHILES,2016,20(4):437-450. http://ir.qdio.ac.cn/handle/337002/130964 doi:10.1007/s00792-016-0834-x Antarctic Sea Ice Freezing Acclimation Horizontal Gene Transfer Psychrophilic Green Microalga Transcriptomic Analysis Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1007/s00792-016-0834-x 2022-06-27T05:37:39Z The Antarctic green alga Chlamydomonas sp. ICE-L was isolated from sea ice. As a psychrophilic microalga, it can tolerate the environmental stress in the sea-ice brine, such as freezing temperature and high salinity. We performed a transcriptome analysis to identify freezing stress responding genes and explore the extreme environmental acclimation-related strategies. Here, we show that many genes in ICE-L transcriptome that encoding PUFA synthesis enzymes, molecular chaperon proteins, and cell membrane transport proteins have high similarity to the gens from Antarctic bacteria. These ICE-L genes are supposed to be acquired through horizontal gene transfer from its symbiotic microbes in the sea-ice brine. The presence of these genes in both sea-ice microalgae and bacteria indicated the biological processes they involved in are possibly contributing to ICE-L success in sea ice. In addition, the biological pathways were compared between ICE-L and its closely related sister species, Chlamydomonas reinhardtii and Volvox carteri. In ICE-L transcripome, many sequences homologous to the plant or bacteria proteins in the post-transcriptional, post-translational modification, and signal-transduction KEGG pathways, are absent in the nonpsychrophilic green algae. These complex structural components might imply enhanced stress adaptation capacity. At last, differential gene expression analysis at the transcriptome level of ICE-L indicated that genes that associated with post-translational modification, lipid metabolism, and nitrogen metabolism are responding to the freezing treatment. In conclusion, the transcriptome of Chlamydomonas sp. ICE-L is very useful for exploring the mutualistic interaction between microalgae and bacteria in sea ice; and discovering the specific genes and metabolism pathways responding to the freezing acclimation in psychrophilic microalgae. The Antarctic green alga Chlamydomonas sp. ICE-L was isolated from sea ice. As a psychrophilic microalga, it can tolerate the environmental stress in the ... Article in Journal/Newspaper Antarc* Antarctic Sea ice Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Antarctic The Antarctic Extremophiles 20 4 437 450 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Antarctic Sea Ice Freezing Acclimation Horizontal Gene Transfer Psychrophilic Green Microalga Transcriptomic Analysis |
spellingShingle |
Antarctic Sea Ice Freezing Acclimation Horizontal Gene Transfer Psychrophilic Green Microalga Transcriptomic Analysis Liu, Chenlin Wang, Xiuliang Wang, Xingna Sun, Chengjun Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis |
topic_facet |
Antarctic Sea Ice Freezing Acclimation Horizontal Gene Transfer Psychrophilic Green Microalga Transcriptomic Analysis |
description |
The Antarctic green alga Chlamydomonas sp. ICE-L was isolated from sea ice. As a psychrophilic microalga, it can tolerate the environmental stress in the sea-ice brine, such as freezing temperature and high salinity. We performed a transcriptome analysis to identify freezing stress responding genes and explore the extreme environmental acclimation-related strategies. Here, we show that many genes in ICE-L transcriptome that encoding PUFA synthesis enzymes, molecular chaperon proteins, and cell membrane transport proteins have high similarity to the gens from Antarctic bacteria. These ICE-L genes are supposed to be acquired through horizontal gene transfer from its symbiotic microbes in the sea-ice brine. The presence of these genes in both sea-ice microalgae and bacteria indicated the biological processes they involved in are possibly contributing to ICE-L success in sea ice. In addition, the biological pathways were compared between ICE-L and its closely related sister species, Chlamydomonas reinhardtii and Volvox carteri. In ICE-L transcripome, many sequences homologous to the plant or bacteria proteins in the post-transcriptional, post-translational modification, and signal-transduction KEGG pathways, are absent in the nonpsychrophilic green algae. These complex structural components might imply enhanced stress adaptation capacity. At last, differential gene expression analysis at the transcriptome level of ICE-L indicated that genes that associated with post-translational modification, lipid metabolism, and nitrogen metabolism are responding to the freezing treatment. In conclusion, the transcriptome of Chlamydomonas sp. ICE-L is very useful for exploring the mutualistic interaction between microalgae and bacteria in sea ice; and discovering the specific genes and metabolism pathways responding to the freezing acclimation in psychrophilic microalgae. The Antarctic green alga Chlamydomonas sp. ICE-L was isolated from sea ice. As a psychrophilic microalga, it can tolerate the environmental stress in the ... |
format |
Article in Journal/Newspaper |
author |
Liu, Chenlin Wang, Xiuliang Wang, Xingna Sun, Chengjun |
author_facet |
Liu, Chenlin Wang, Xiuliang Wang, Xingna Sun, Chengjun |
author_sort |
Liu, Chenlin |
title |
Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis |
title_short |
Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis |
title_full |
Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis |
title_fullStr |
Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis |
title_full_unstemmed |
Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis |
title_sort |
acclimation of antarctic chlamydomonas to the sea-ice environment: a transcriptomic analysis |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/130964 https://doi.org/10.1007/s00792-016-0834-x |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Sea ice |
genre_facet |
Antarc* Antarctic Sea ice |
op_relation |
EXTREMOPHILES Liu, Chenlin,Wang, Xiuliang,Wang, Xingna,et al. Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis[J]. EXTREMOPHILES,2016,20(4):437-450. http://ir.qdio.ac.cn/handle/337002/130964 doi:10.1007/s00792-016-0834-x |
op_doi |
https://doi.org/10.1007/s00792-016-0834-x |
container_title |
Extremophiles |
container_volume |
20 |
container_issue |
4 |
container_start_page |
437 |
op_container_end_page |
450 |
_version_ |
1766137550132477952 |