An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2
Nuclear factor (NF)-kappa B pathway is an evolutionally conserved pathway in activating immune response, in which I kappa Bs can repress the activation. In the present study, cgi-miR-2d, an invertebrate-specific microRNA, was proved to regulate CgI kappa B2 expression and haemocyte phagocytosis duri...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/130961 2023-05-15T15:58:49+02:00 An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 Chen, Hao Zhou, Zhi Wang, Hao Wang, Lingling Wang, Weilin Liu, Rui Qiu, Limei Song, Linsheng 2016-07-12 http://ir.qdio.ac.cn/handle/337002/130961 https://doi.org/10.1038/srep29591 英语 eng SCIENTIFIC REPORTS Chen, Hao,Zhou, Zhi,Wang, Hao,et al. An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2[J]. SCIENTIFIC REPORTS,2016,6. http://ir.qdio.ac.cn/handle/337002/130961 doi:10.1038/srep29591 Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1038/srep29591 2022-06-27T05:37:39Z Nuclear factor (NF)-kappa B pathway is an evolutionally conserved pathway in activating immune response, in which I kappa Bs can repress the activation. In the present study, cgi-miR-2d, an invertebrate-specific microRNA, was proved to regulate CgI kappa B2 expression and haemocyte phagocytosis during bacterial infection in oyster Crassostrea gigas. The expression of cgi-miR-2d was significantly up-regulated after Vibrio splendidus challenge, while CgI kappa B2 transcripts decreased. Significant decreases in both luminescence and CgI kappa B2 3'UTR level was observed after transfection of cgi-miR-2d in CgI kappa B2 3'UTR luciferase reporter assay. CgI kappa B2 mRNA level decreased significantly (0.51-fold of control group, p < 0.05) in gain-of-function assay of cgi-miR-2d in vivo while it increased markedly (1.27-fold, p < 0.05) when cgi-miR-2d was repressed (0.10-fold, p < 0.01). A significant increase of haemocyte phagocytosis rate was observed in cgi-miR-2d overexpression group (p < 0.01), consistent with results in CgI kappa B2 knock-down group (p < 0.01). Moreover, the apoptosis rate of haemocytes was found significantly declined (28.57%, p < 0.01) in gain-of-function assay of cgi-miR-2d. Together, those results not only depicted the functional conservation of miR-2d family in anti-apoptosis of oysters but also highlighted its interaction with phagocytosis by modulating NF-kappa B pathway, which might dedicate critically to the well-balance of host immune response. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Scientific Reports 6 1 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
description |
Nuclear factor (NF)-kappa B pathway is an evolutionally conserved pathway in activating immune response, in which I kappa Bs can repress the activation. In the present study, cgi-miR-2d, an invertebrate-specific microRNA, was proved to regulate CgI kappa B2 expression and haemocyte phagocytosis during bacterial infection in oyster Crassostrea gigas. The expression of cgi-miR-2d was significantly up-regulated after Vibrio splendidus challenge, while CgI kappa B2 transcripts decreased. Significant decreases in both luminescence and CgI kappa B2 3'UTR level was observed after transfection of cgi-miR-2d in CgI kappa B2 3'UTR luciferase reporter assay. CgI kappa B2 mRNA level decreased significantly (0.51-fold of control group, p < 0.05) in gain-of-function assay of cgi-miR-2d in vivo while it increased markedly (1.27-fold, p < 0.05) when cgi-miR-2d was repressed (0.10-fold, p < 0.01). A significant increase of haemocyte phagocytosis rate was observed in cgi-miR-2d overexpression group (p < 0.01), consistent with results in CgI kappa B2 knock-down group (p < 0.01). Moreover, the apoptosis rate of haemocytes was found significantly declined (28.57%, p < 0.01) in gain-of-function assay of cgi-miR-2d. Together, those results not only depicted the functional conservation of miR-2d family in anti-apoptosis of oysters but also highlighted its interaction with phagocytosis by modulating NF-kappa B pathway, which might dedicate critically to the well-balance of host immune response. |
format |
Article in Journal/Newspaper |
author |
Chen, Hao Zhou, Zhi Wang, Hao Wang, Lingling Wang, Weilin Liu, Rui Qiu, Limei Song, Linsheng |
spellingShingle |
Chen, Hao Zhou, Zhi Wang, Hao Wang, Lingling Wang, Weilin Liu, Rui Qiu, Limei Song, Linsheng An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 |
author_facet |
Chen, Hao Zhou, Zhi Wang, Hao Wang, Lingling Wang, Weilin Liu, Rui Qiu, Limei Song, Linsheng |
author_sort |
Chen, Hao |
title |
An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 |
title_short |
An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 |
title_full |
An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 |
title_fullStr |
An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 |
title_full_unstemmed |
An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2 |
title_sort |
invertebrate-specific and immune-responsive microrna augments oyster haemocyte phagocytosis by targeting cgi kappa b2 |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/130961 https://doi.org/10.1038/srep29591 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
SCIENTIFIC REPORTS Chen, Hao,Zhou, Zhi,Wang, Hao,et al. An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgI kappa B2[J]. SCIENTIFIC REPORTS,2016,6. http://ir.qdio.ac.cn/handle/337002/130961 doi:10.1038/srep29591 |
op_doi |
https://doi.org/10.1038/srep29591 |
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Scientific Reports |
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6 |
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1 |
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1766394591902171136 |