A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas
Glutamic acid decarboxylase (GAD), a rate-limiting enzyme to catalyze the reaction converting the excitatory neurotransmitter glutamate to inhibitory neurotransmitter gamma-aminobutyric acid (GABA), not only functions in nervous system, but also plays important roles in immunomodulation in vertebrat...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/130896 https://doi.org/10.1016/j.dci.2016.05.010 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/130896 2023-05-15T15:57:39+02:00 A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas Li, Meijia Wang, Lingling Qiu, Limei Wang, Weilin Xin, Lusheng Xu, Jiachao Wang, Hao Song, Linsheng 2016-10-01 http://ir.qdio.ac.cn/handle/337002/130896 https://doi.org/10.1016/j.dci.2016.05.010 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Li, Meijia,Wang, Lingling,Qiu, Limei,et al. A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,63:56-65. http://ir.qdio.ac.cn/handle/337002/130896 doi:10.1016/j.dci.2016.05.010 Crassostrea Gigas Gaba Synthase Immune Response Hemocyte Specific Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.dci.2016.05.010 2022-06-27T05:37:35Z Glutamic acid decarboxylase (GAD), a rate-limiting enzyme to catalyze the reaction converting the excitatory neurotransmitter glutamate to inhibitory neurotransmitter gamma-aminobutyric acid (GABA), not only functions in nervous system, but also plays important roles in immunomodulation in vertebrates. However, GAD has rarely been reported in invertebrates, and never in molluscs. In the present study, one GAD homologue (designed as CgGAD) was identified from Pacific oyster Crassostrea gigas. The full length cDNA of CgGAD was 1689 bp encoding a polypeptide of 562 amino acids containing a conserved pyridoxal-dependent decarboxylase domain. CgGAD mRNA and protein could be detected in ganglion and hemocytes of oysters, and their abundance in hemocytes was unexpectedly much higher than those in ganglion. More importantly, CgGAD was mostly located in those granulocytes without phagocytic capacity in oysters, and could dynamically respond to LPS stimulation. Further, after being transfected into HEK293 cells, CgGAD could promote the production of GABA. Collectively, these findings suggested that CgGAD, as a GABA synthase and molecular marker of GABAergic system, was mainly distributed in hemocytes and ganglion and involved in neuroendocrine-immune regulation network in oysters, which also provided a novel insight to the co-evolution between nervous system and immune system. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 63 56 65 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Gaba Synthase Immune Response Hemocyte Specific |
spellingShingle |
Crassostrea Gigas Gaba Synthase Immune Response Hemocyte Specific Li, Meijia Wang, Lingling Qiu, Limei Wang, Weilin Xin, Lusheng Xu, Jiachao Wang, Hao Song, Linsheng A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea Gigas Gaba Synthase Immune Response Hemocyte Specific |
description |
Glutamic acid decarboxylase (GAD), a rate-limiting enzyme to catalyze the reaction converting the excitatory neurotransmitter glutamate to inhibitory neurotransmitter gamma-aminobutyric acid (GABA), not only functions in nervous system, but also plays important roles in immunomodulation in vertebrates. However, GAD has rarely been reported in invertebrates, and never in molluscs. In the present study, one GAD homologue (designed as CgGAD) was identified from Pacific oyster Crassostrea gigas. The full length cDNA of CgGAD was 1689 bp encoding a polypeptide of 562 amino acids containing a conserved pyridoxal-dependent decarboxylase domain. CgGAD mRNA and protein could be detected in ganglion and hemocytes of oysters, and their abundance in hemocytes was unexpectedly much higher than those in ganglion. More importantly, CgGAD was mostly located in those granulocytes without phagocytic capacity in oysters, and could dynamically respond to LPS stimulation. Further, after being transfected into HEK293 cells, CgGAD could promote the production of GABA. Collectively, these findings suggested that CgGAD, as a GABA synthase and molecular marker of GABAergic system, was mainly distributed in hemocytes and ganglion and involved in neuroendocrine-immune regulation network in oysters, which also provided a novel insight to the co-evolution between nervous system and immune system. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Li, Meijia Wang, Lingling Qiu, Limei Wang, Weilin Xin, Lusheng Xu, Jiachao Wang, Hao Song, Linsheng |
author_facet |
Li, Meijia Wang, Lingling Qiu, Limei Wang, Weilin Xin, Lusheng Xu, Jiachao Wang, Hao Song, Linsheng |
author_sort |
Li, Meijia |
title |
A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas |
title_short |
A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas |
title_full |
A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas |
title_fullStr |
A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas |
title_full_unstemmed |
A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas |
title_sort |
glutamic acid decarboxylase (cggad) highly expressed in hemocytes of pacific oyster crassostrea gigas |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/130896 https://doi.org/10.1016/j.dci.2016.05.010 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Li, Meijia,Wang, Lingling,Qiu, Limei,et al. A glutamic acid decarboxylase (CgGAD) highly expressed in hemocytes of Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,63:56-65. http://ir.qdio.ac.cn/handle/337002/130896 doi:10.1016/j.dci.2016.05.010 |
op_doi |
https://doi.org/10.1016/j.dci.2016.05.010 |
container_title |
Developmental & Comparative Immunology |
container_volume |
63 |
container_start_page |
56 |
op_container_end_page |
65 |
_version_ |
1766393324337364992 |