Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination
The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantl...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/126271 2023-05-15T18:15:43+02:00 Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination Wang, Yujuan Wang, Xiuhua Huang, Jie Li, Jun 2016-03-01 http://ir.qdio.ac.cn/handle/337002/126271 https://doi.org/10.3390/ijms17030325 英语 eng INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Wang, Yujuan,Wang, Xiuhua,Huang, Jie,et al. Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(3). http://ir.qdio.ac.cn/handle/337002/126271 doi:10.3390/ijms17030325 Vaccination Relative Percent Of Survival (Rps) Quillaja Saponaria Saponins Scophthalmus Maximus Adjuvant Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.3390/ijms17030325 2022-06-27T05:37:35Z The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 +/- 13.6)%, (81.7 +/- 8.2)%, (77.8 +/- 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR International Journal of Molecular Sciences 17 3 325 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Vaccination Relative Percent Of Survival (Rps) Quillaja Saponaria Saponins Scophthalmus Maximus Adjuvant |
spellingShingle |
Vaccination Relative Percent Of Survival (Rps) Quillaja Saponaria Saponins Scophthalmus Maximus Adjuvant Wang, Yujuan Wang, Xiuhua Huang, Jie Li, Jun Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
topic_facet |
Vaccination Relative Percent Of Survival (Rps) Quillaja Saponaria Saponins Scophthalmus Maximus Adjuvant |
description |
The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 +/- 13.6)%, (81.7 +/- 8.2)%, (77.8 +/- 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route. |
format |
Article in Journal/Newspaper |
author |
Wang, Yujuan Wang, Xiuhua Huang, Jie Li, Jun |
author_facet |
Wang, Yujuan Wang, Xiuhua Huang, Jie Li, Jun |
author_sort |
Wang, Yujuan |
title |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_short |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_full |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_fullStr |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_full_unstemmed |
Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination |
title_sort |
adjuvant effect of quillaja saponaria saponin (qss) on protective efficacy and igm generation in turbot (scophthalmus maximus) upon immersion vaccination |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/126271 https://doi.org/10.3390/ijms17030325 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Wang, Yujuan,Wang, Xiuhua,Huang, Jie,et al. Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(3). http://ir.qdio.ac.cn/handle/337002/126271 doi:10.3390/ijms17030325 |
op_doi |
https://doi.org/10.3390/ijms17030325 |
container_title |
International Journal of Molecular Sciences |
container_volume |
17 |
container_issue |
3 |
container_start_page |
325 |
_version_ |
1766188908917293056 |