Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas
Apoptosis is a form of programmed cell death process controlled by a family of cysteine proteases called caspases, which plays a crucial role in the immune system homeostasis. The apoptosis and the detailed regulation mechanism have been well studied in vertebrate, but the information in lower anima...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/126232 https://doi.org/10.1016/j.dci.2016.03.015 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/126232 2023-05-15T15:57:50+02:00 Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas Xu, Jiachao Jiang, Shuai Li, Yiqun Li, Meijia Cheng, Qi Zhao, Depeng Yang, Bin Jia, Zhihao Wang, Lingling Song, Linsheng 2016-08-01 http://ir.qdio.ac.cn/handle/337002/126232 https://doi.org/10.1016/j.dci.2016.03.015 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Xu, Jiachao,Jiang, Shuai,Li, Yiqun,et al. Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,61:1-12. http://ir.qdio.ac.cn/handle/337002/126232 doi:10.1016/j.dci.2016.03.015 Crassostrea Gigas Caspase-3 Intracellular Immune Receptor Lps Immune Response Apoptosis Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.dci.2016.03.015 2022-06-27T05:37:35Z Apoptosis is a form of programmed cell death process controlled by a family of cysteine proteases called caspases, which plays a crucial role in the immune system homeostasis. The apoptosis and the detailed regulation mechanism have been well studied in vertebrate, but the information in lower animals, especially invertebrates, is still very limited. In the present study, Caspase-3 in the Pacific oyster Crassostrea gigas (designated CgCaspase-3) was enriched by lipopolysaccharide (LPS) affinity chromatography and further identified by MALDI-TOF/TOF-mass spectrometry. The binding activity of CgCaspase-3 to LPS was confirmed by enzyme-linked immunosorbent assay, and surface plasmon resonance analysis revealed its high binding specificity and moderate binding affinity (K-D = 1.08 x 10(-6) M) to LPS. The recombinant CgCaspase-3 exhibited high proteolytic activity to substrate Ac-DEVD-pNA and relatively weak activity to substrate Ac-DMQD-pNA and Ac-VDQQD-pNA. The binding of CgCaspase-3 to LPS significantly inhibited its proteolytic activity toward AC-DEVD-pNA in vitro. The over-expression of CgCaspase-3 leaded to the phosphatidylserine exposure on the external plasma membrane and the cleavage of poly (ADP-ribose) polymerase, which reduced cell viability, and finally induced cell apoptosis. But the cell apoptosis mediated by CgCaspase-3 in vivo was significantly inhibited by the treatment of LPS. These results collectively indicated that CgCaspase-3 could serve as an intracellular LPS receptor, and the interaction of LPS with CgCaspase-3 specifically inhibited the cell apoptosis induced by CgCaspase-3. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 61 1 12 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Caspase-3 Intracellular Immune Receptor Lps Immune Response Apoptosis |
spellingShingle |
Crassostrea Gigas Caspase-3 Intracellular Immune Receptor Lps Immune Response Apoptosis Xu, Jiachao Jiang, Shuai Li, Yiqun Li, Meijia Cheng, Qi Zhao, Depeng Yang, Bin Jia, Zhihao Wang, Lingling Song, Linsheng Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas |
topic_facet |
Crassostrea Gigas Caspase-3 Intracellular Immune Receptor Lps Immune Response Apoptosis |
description |
Apoptosis is a form of programmed cell death process controlled by a family of cysteine proteases called caspases, which plays a crucial role in the immune system homeostasis. The apoptosis and the detailed regulation mechanism have been well studied in vertebrate, but the information in lower animals, especially invertebrates, is still very limited. In the present study, Caspase-3 in the Pacific oyster Crassostrea gigas (designated CgCaspase-3) was enriched by lipopolysaccharide (LPS) affinity chromatography and further identified by MALDI-TOF/TOF-mass spectrometry. The binding activity of CgCaspase-3 to LPS was confirmed by enzyme-linked immunosorbent assay, and surface plasmon resonance analysis revealed its high binding specificity and moderate binding affinity (K-D = 1.08 x 10(-6) M) to LPS. The recombinant CgCaspase-3 exhibited high proteolytic activity to substrate Ac-DEVD-pNA and relatively weak activity to substrate Ac-DMQD-pNA and Ac-VDQQD-pNA. The binding of CgCaspase-3 to LPS significantly inhibited its proteolytic activity toward AC-DEVD-pNA in vitro. The over-expression of CgCaspase-3 leaded to the phosphatidylserine exposure on the external plasma membrane and the cleavage of poly (ADP-ribose) polymerase, which reduced cell viability, and finally induced cell apoptosis. But the cell apoptosis mediated by CgCaspase-3 in vivo was significantly inhibited by the treatment of LPS. These results collectively indicated that CgCaspase-3 could serve as an intracellular LPS receptor, and the interaction of LPS with CgCaspase-3 specifically inhibited the cell apoptosis induced by CgCaspase-3. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Xu, Jiachao Jiang, Shuai Li, Yiqun Li, Meijia Cheng, Qi Zhao, Depeng Yang, Bin Jia, Zhihao Wang, Lingling Song, Linsheng |
author_facet |
Xu, Jiachao Jiang, Shuai Li, Yiqun Li, Meijia Cheng, Qi Zhao, Depeng Yang, Bin Jia, Zhihao Wang, Lingling Song, Linsheng |
author_sort |
Xu, Jiachao |
title |
Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas |
title_short |
Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas |
title_full |
Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas |
title_fullStr |
Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas |
title_full_unstemmed |
Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas |
title_sort |
caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster crassostrea gigas |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/126232 https://doi.org/10.1016/j.dci.2016.03.015 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Xu, Jiachao,Jiang, Shuai,Li, Yiqun,et al. Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,61:1-12. http://ir.qdio.ac.cn/handle/337002/126232 doi:10.1016/j.dci.2016.03.015 |
op_doi |
https://doi.org/10.1016/j.dci.2016.03.015 |
container_title |
Developmental & Comparative Immunology |
container_volume |
61 |
container_start_page |
1 |
op_container_end_page |
12 |
_version_ |
1766393528421711872 |