A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release
Sialic acid binding immunoglobulin-type lectin (siglec) belongs to the immunoglobulin superfamily (IgSF), which acts as regulator involved in glycan recognition and signal transduction in the immune and nervous systems. In the present study, a siglec gene (designated CgSiglec-1) was characterized fr...
Published in: | Developmental & Comparative Immunology |
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Language: | English |
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2016
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Online Access: | http://ir.qdio.ac.cn/handle/337002/126230 https://doi.org/10.1016/j.dci.2016.03.026 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/126230 2023-05-15T15:58:19+02:00 A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release Liu, Conghui Jiang, Shuai Wang, Mengqiang Wang, Lingling Chen, Hao Xu, Jiachao Lv, Zhao Song, Linsheng 2016-08-01 http://ir.qdio.ac.cn/handle/337002/126230 https://doi.org/10.1016/j.dci.2016.03.026 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Liu, Conghui,Jiang, Shuai,Wang, Mengqiang,et al. A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,61:136-144. http://ir.qdio.ac.cn/handle/337002/126230 doi:10.1016/j.dci.2016.03.026 Sialic Acid Binding Immunoglobulin-type Lectins Innate Immune Response Recognition Spectrum Cascading Inhibitory Phagocytosis Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.dci.2016.03.026 2022-06-27T05:37:35Z Sialic acid binding immunoglobulin-type lectin (siglec) belongs to the immunoglobulin superfamily (IgSF), which acts as regulator involved in glycan recognition and signal transduction in the immune and nervous systems. In the present study, a siglec gene (designated CgSiglec-1) was characterized from the Pacific oyster, Crassostrea gigas. The cDNA of CgSiglec-1 was of 1251 bp encoding a predicted polypeptide of 416 amino acids. CgSiglec-1 was composed of two I-set immunoglobulin (Ig) domains, one trans membrane (TM) domain and two ITIM motifs, sharing a sequence similarity with vertebrate CD22 homologs. The mRNA expression of CgSiglec-1 could be detected in all the selected tissues, with the highest level in hemocytes and labial palps. The confocal analysis revealed that CgSiglec-1 mainly distributed on the cytoplasmic membrane of the oyster hemocytes. In addition, the mRNA transcripts of CgSiglec-1 in hemocytes increased significantly (4.29-fold to that of control group, p < 0.05) after Vibrio splendidus stimulation. The recombinant CgSiglec-1 protein (rCgSiglec-1) could bind to poly sialic acid (pSIAS), lipopolysaccharides (LPS) and peptidoglycan (PGN) in a dose-dependent manner. The blockade of CgSiglec-1 by specific polyclonal antibodies could enhance the LPS-induced cell apoptosis, phagocytosis towards V. splendidus and the release of cytokines, such as CgTNF-1, CgIFNLP and CgIL-17. The results collectively indicated that CgSiglec-1 could act as a bridge molecule between invader recognition and signal transduction cascade, and modulate the immune response by inhibiting various important processes of immunity in oyster. (C) 2016 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 61 136 144 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Sialic Acid Binding Immunoglobulin-type Lectins Innate Immune Response Recognition Spectrum Cascading Inhibitory Phagocytosis |
spellingShingle |
Sialic Acid Binding Immunoglobulin-type Lectins Innate Immune Response Recognition Spectrum Cascading Inhibitory Phagocytosis Liu, Conghui Jiang, Shuai Wang, Mengqiang Wang, Lingling Chen, Hao Xu, Jiachao Lv, Zhao Song, Linsheng A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release |
topic_facet |
Sialic Acid Binding Immunoglobulin-type Lectins Innate Immune Response Recognition Spectrum Cascading Inhibitory Phagocytosis |
description |
Sialic acid binding immunoglobulin-type lectin (siglec) belongs to the immunoglobulin superfamily (IgSF), which acts as regulator involved in glycan recognition and signal transduction in the immune and nervous systems. In the present study, a siglec gene (designated CgSiglec-1) was characterized from the Pacific oyster, Crassostrea gigas. The cDNA of CgSiglec-1 was of 1251 bp encoding a predicted polypeptide of 416 amino acids. CgSiglec-1 was composed of two I-set immunoglobulin (Ig) domains, one trans membrane (TM) domain and two ITIM motifs, sharing a sequence similarity with vertebrate CD22 homologs. The mRNA expression of CgSiglec-1 could be detected in all the selected tissues, with the highest level in hemocytes and labial palps. The confocal analysis revealed that CgSiglec-1 mainly distributed on the cytoplasmic membrane of the oyster hemocytes. In addition, the mRNA transcripts of CgSiglec-1 in hemocytes increased significantly (4.29-fold to that of control group, p < 0.05) after Vibrio splendidus stimulation. The recombinant CgSiglec-1 protein (rCgSiglec-1) could bind to poly sialic acid (pSIAS), lipopolysaccharides (LPS) and peptidoglycan (PGN) in a dose-dependent manner. The blockade of CgSiglec-1 by specific polyclonal antibodies could enhance the LPS-induced cell apoptosis, phagocytosis towards V. splendidus and the release of cytokines, such as CgTNF-1, CgIFNLP and CgIL-17. The results collectively indicated that CgSiglec-1 could act as a bridge molecule between invader recognition and signal transduction cascade, and modulate the immune response by inhibiting various important processes of immunity in oyster. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Liu, Conghui Jiang, Shuai Wang, Mengqiang Wang, Lingling Chen, Hao Xu, Jiachao Lv, Zhao Song, Linsheng |
author_facet |
Liu, Conghui Jiang, Shuai Wang, Mengqiang Wang, Lingling Chen, Hao Xu, Jiachao Lv, Zhao Song, Linsheng |
author_sort |
Liu, Conghui |
title |
A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release |
title_short |
A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release |
title_full |
A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release |
title_fullStr |
A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release |
title_full_unstemmed |
A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release |
title_sort |
novel siglec (cgsiglec-1) from the pacific oyster (crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/126230 https://doi.org/10.1016/j.dci.2016.03.026 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Liu, Conghui,Jiang, Shuai,Wang, Mengqiang,et al. A novel siglec (CgSiglec-1) from the Pacific oyster (Crassostrea gigas) with broad recognition spectrum and inhibitory activity to apoptosis, phagocytosis and cytokine release[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,61:136-144. http://ir.qdio.ac.cn/handle/337002/126230 doi:10.1016/j.dci.2016.03.026 |
op_doi |
https://doi.org/10.1016/j.dci.2016.03.026 |
container_title |
Developmental & Comparative Immunology |
container_volume |
61 |
container_start_page |
136 |
op_container_end_page |
144 |
_version_ |
1766394044002336768 |