A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder
Streptococcosis and vibriosis caused by Streptococcus iniae and Vibrio anguillarum respectively have affected fish culture industries around the world. Previous studies have indicated that the S. iniae antigen Sia10 and the V. anguillarum outer membrane protein OmpU, when used as DNA vaccines, induc...
Published in: | Fish & Shellfish Immunology |
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2012
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Online Access: | http://ir.qdio.ac.cn/handle/337002/12256 https://doi.org/10.1016/j.fsi.2012.03.024 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/12256 2023-05-15T18:15:53+02:00 A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder Sun, Yun Zhang, Min Liu, Chun-sheng Qiu, Reng Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. 2012-06-01 http://ir.qdio.ac.cn/handle/337002/12256 https://doi.org/10.1016/j.fsi.2012.03.024 英语 eng FISH & SHELLFISH IMMUNOLOGY Sun, Yun; Zhang, Min; Liu, Chunsheng; Qiu, Reng; Sun, Li.A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder,FISH & SHELLFISH IMMUNOLOGY,2012,32(6):1216-1222 http://ir.qdio.ac.cn/handle/337002/12256 doi:10.1016/j.fsi.2012.03.024 Streptococcus Iniae Vibrio Anguillarum Paralichthys Olivaceus Dna Vaccine Cross Protection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Article 期刊论文 2012 ftchinacasciocas https://doi.org/10.1016/j.fsi.2012.03.024 2022-06-27T05:34:23Z Streptococcosis and vibriosis caused by Streptococcus iniae and Vibrio anguillarum respectively have affected fish culture industries around the world. Previous studies have indicated that the S. iniae antigen Sia10 and the V. anguillarum outer membrane protein OmpU, when used as DNA vaccines, induce protection in turbot (Scophthalmus maximus) and Asian seabass (Lates calcarifer) respectively. In this study, with an effort to develop effective vaccines against S. iniae and V. anguillarum, we constructed three DNA vaccines based on Sia10 and OmpU and examined their immune effects in a model of Japanese flounder (Paralichthys olivaceus), which in China is known to suffer from both streptococcosis and vibriosis. Of the three DNA vaccines constructed in this study, pIDSia10 and pIDOmpU express Sia10 and OmpU respectively, while pSiVal expresses Sia10 and OmpU as two individual proteins translated from a single bicistronic mRNA transcript. At 7 and 28 days post-vaccination, vaccine plasmids and expression of the vaccine-encoding genes were detected in the muscle, spleen, kidney, and liver of the vaccinated fish. lmmunocolloidal gold electron microscopy detected production of Sia10 and OmpU proteins in pIDSia10- and pIDOmpU-vaccinated fish respectively, while both Sia10 and OmpU proteins were detected in pSiVa1-vaccinated fish. At one and two months post-vaccination, fish vaccinated with pIDSia10 and pSiVa1 exhibited comparable relative percent of survival (RPS) rates (80%-87%) following lethal S. iniae challenge. Similar protection rates were produced by fish vaccinated with pIDOmpU and pSiVal following lethal V. anguillarum challenge. Immunological analysis showed that (i) all vaccines induced specific serum antibody production which enhanced complement-mediated bactericidal activity, and (ii) pSiVal modulated the expression of a wide spectrum of immune relevant genes in a time-dependent manner. Together these results indicate that pSiVal is an effective bivalent vaccine that induces strong cross protection in ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 32 6 1216 1222 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Streptococcus Iniae Vibrio Anguillarum Paralichthys Olivaceus Dna Vaccine Cross Protection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Streptococcus Iniae Vibrio Anguillarum Paralichthys Olivaceus Dna Vaccine Cross Protection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Sun, Yun Zhang, Min Liu, Chun-sheng Qiu, Reng Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder |
topic_facet |
Streptococcus Iniae Vibrio Anguillarum Paralichthys Olivaceus Dna Vaccine Cross Protection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Streptococcosis and vibriosis caused by Streptococcus iniae and Vibrio anguillarum respectively have affected fish culture industries around the world. Previous studies have indicated that the S. iniae antigen Sia10 and the V. anguillarum outer membrane protein OmpU, when used as DNA vaccines, induce protection in turbot (Scophthalmus maximus) and Asian seabass (Lates calcarifer) respectively. In this study, with an effort to develop effective vaccines against S. iniae and V. anguillarum, we constructed three DNA vaccines based on Sia10 and OmpU and examined their immune effects in a model of Japanese flounder (Paralichthys olivaceus), which in China is known to suffer from both streptococcosis and vibriosis. Of the three DNA vaccines constructed in this study, pIDSia10 and pIDOmpU express Sia10 and OmpU respectively, while pSiVal expresses Sia10 and OmpU as two individual proteins translated from a single bicistronic mRNA transcript. At 7 and 28 days post-vaccination, vaccine plasmids and expression of the vaccine-encoding genes were detected in the muscle, spleen, kidney, and liver of the vaccinated fish. lmmunocolloidal gold electron microscopy detected production of Sia10 and OmpU proteins in pIDSia10- and pIDOmpU-vaccinated fish respectively, while both Sia10 and OmpU proteins were detected in pSiVa1-vaccinated fish. At one and two months post-vaccination, fish vaccinated with pIDSia10 and pSiVa1 exhibited comparable relative percent of survival (RPS) rates (80%-87%) following lethal S. iniae challenge. Similar protection rates were produced by fish vaccinated with pIDOmpU and pSiVal following lethal V. anguillarum challenge. Immunological analysis showed that (i) all vaccines induced specific serum antibody production which enhanced complement-mediated bactericidal activity, and (ii) pSiVal modulated the expression of a wide spectrum of immune relevant genes in a time-dependent manner. Together these results indicate that pSiVal is an effective bivalent vaccine that induces strong cross protection in ... |
format |
Article in Journal/Newspaper |
author |
Sun, Yun Zhang, Min Liu, Chun-sheng Qiu, Reng Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_facet |
Sun, Yun Zhang, Min Liu, Chun-sheng Qiu, Reng Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_sort |
Sun, Yun |
title |
A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder |
title_short |
A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder |
title_full |
A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder |
title_fullStr |
A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder |
title_full_unstemmed |
A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder |
title_sort |
divalent dna vaccine based on sia10 and ompu induces cross protection against streptococcus iniae and vibrio anguillarum in japanese flounder |
publishDate |
2012 |
url |
http://ir.qdio.ac.cn/handle/337002/12256 https://doi.org/10.1016/j.fsi.2012.03.024 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Sun, Yun; Zhang, Min; Liu, Chunsheng; Qiu, Reng; Sun, Li.A divalent DNA vaccine based on Sia10 and OmpU induces cross protection against Streptococcus iniae and Vibrio anguillarum in Japanese flounder,FISH & SHELLFISH IMMUNOLOGY,2012,32(6):1216-1222 http://ir.qdio.ac.cn/handle/337002/12256 doi:10.1016/j.fsi.2012.03.024 |
op_doi |
https://doi.org/10.1016/j.fsi.2012.03.024 |
container_title |
Fish & Shellfish Immunology |
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32 |
container_issue |
6 |
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1216 |
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1222 |
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1766189128025636864 |