Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas
Fibrinogen-related proteins (FREPs) are lectins with at least a fibrinogen-like domain. They are widespread in Mollusca and play an important role as immune pattern-recognition receptors. These diverse molecules provide interesting models for the study of the evolution of innate immunity. Although o...
Published in: | Chinese Science Bulletin |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2012
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/12245 https://doi.org/10.1007/s11434-012-5155-6 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/12245 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/12245 2023-05-15T15:57:50+02:00 Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas Zhang LinLin Li Li Zhang GuoFan Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. 2012-09-01 http://ir.qdio.ac.cn/handle/337002/12245 https://doi.org/10.1007/s11434-012-5155-6 英语 eng CHINESE SCIENCE BULLETIN Zhang LinLin; Li Li; Zhang GuoFan.Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas,CHINESE SCIENCE BULLETIN,2012,57(25):3312-3319 http://ir.qdio.ac.cn/handle/337002/12245 doi:10.1007/s11434-012-5155-6 Crassostrea Gigas Freps Fibrinogen-related Proteins Recombination Oysters Multidisciplinary Sciences Article 期刊论文 2012 ftchinacasciocas https://doi.org/10.1007/s11434-012-5155-6 2022-06-27T05:34:23Z Fibrinogen-related proteins (FREPs) are lectins with at least a fibrinogen-like domain. They are widespread in Mollusca and play an important role as immune pattern-recognition receptors. These diverse molecules provide interesting models for the study of the evolution of innate immunity. Although oysters are among the most studied mollusks, there have been few investigations of this gene family in oysters. This paper reports the cloning of the first oyster FREP, named CgFREP, from the Pacific oyster Crassostrea gigas. Five types of FREP (CgFREP-1 to CgFREP-5) were obtained based on a single pair of primers, confirming their high diversity. The proteins deduced from these FREPs contained 253-363 amino acids and were divided into three subfamilies based on sequence identities (cutoff point < 86%). The topological structure of the phylogenetic tree showed the five CgFREPs clustered together and were completely separated from the subgroup of FREPs obtained from Biomphalaria glabrata (BgFREPs). This implies that FREP diversity evolved before the BgFREPs branched off. An interesting aspect of the CgFREPs was their individual hypermutation. Ninety-three SNPs and five indels were characterized among CgFREPs. Some mutations (e.g. an insertion in CgFREP-5) could cause premature translation termination. Multiple alignments suggest recombination events might have occurred during exchanges between alleles, or by somatic diversification, or both. Individual sequence variability parallels the extraordinary complexity of the innate immune system, suggesting a potential capacity to recognize and eliminate many kinds of pathogens, even in a primitive system. The diversity of immune pattern-recognition receptors may offer an alternative strategy that functions in the absence of highly specific immune globulins, which only emerged in vertebrates. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Chinese Science Bulletin 57 25 3312 3319 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Freps Fibrinogen-related Proteins Recombination Oysters Multidisciplinary Sciences |
spellingShingle |
Crassostrea Gigas Freps Fibrinogen-related Proteins Recombination Oysters Multidisciplinary Sciences Zhang LinLin Li Li Zhang GuoFan Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas |
topic_facet |
Crassostrea Gigas Freps Fibrinogen-related Proteins Recombination Oysters Multidisciplinary Sciences |
description |
Fibrinogen-related proteins (FREPs) are lectins with at least a fibrinogen-like domain. They are widespread in Mollusca and play an important role as immune pattern-recognition receptors. These diverse molecules provide interesting models for the study of the evolution of innate immunity. Although oysters are among the most studied mollusks, there have been few investigations of this gene family in oysters. This paper reports the cloning of the first oyster FREP, named CgFREP, from the Pacific oyster Crassostrea gigas. Five types of FREP (CgFREP-1 to CgFREP-5) were obtained based on a single pair of primers, confirming their high diversity. The proteins deduced from these FREPs contained 253-363 amino acids and were divided into three subfamilies based on sequence identities (cutoff point < 86%). The topological structure of the phylogenetic tree showed the five CgFREPs clustered together and were completely separated from the subgroup of FREPs obtained from Biomphalaria glabrata (BgFREPs). This implies that FREP diversity evolved before the BgFREPs branched off. An interesting aspect of the CgFREPs was their individual hypermutation. Ninety-three SNPs and five indels were characterized among CgFREPs. Some mutations (e.g. an insertion in CgFREP-5) could cause premature translation termination. Multiple alignments suggest recombination events might have occurred during exchanges between alleles, or by somatic diversification, or both. Individual sequence variability parallels the extraordinary complexity of the innate immune system, suggesting a potential capacity to recognize and eliminate many kinds of pathogens, even in a primitive system. The diversity of immune pattern-recognition receptors may offer an alternative strategy that functions in the absence of highly specific immune globulins, which only emerged in vertebrates. |
format |
Article in Journal/Newspaper |
author |
Zhang LinLin Li Li Zhang GuoFan Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
author_facet |
Zhang LinLin Li Li Zhang GuoFan Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
author_sort |
Zhang LinLin |
title |
Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas |
title_short |
Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas |
title_full |
Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas |
title_fullStr |
Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas |
title_full_unstemmed |
Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas |
title_sort |
sequence variability of fibrinogen-related proteins (freps) in crassostrea gigas |
publishDate |
2012 |
url |
http://ir.qdio.ac.cn/handle/337002/12245 https://doi.org/10.1007/s11434-012-5155-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
CHINESE SCIENCE BULLETIN Zhang LinLin; Li Li; Zhang GuoFan.Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas,CHINESE SCIENCE BULLETIN,2012,57(25):3312-3319 http://ir.qdio.ac.cn/handle/337002/12245 doi:10.1007/s11434-012-5155-6 |
op_doi |
https://doi.org/10.1007/s11434-012-5155-6 |
container_title |
Chinese Science Bulletin |
container_volume |
57 |
container_issue |
25 |
container_start_page |
3312 |
op_container_end_page |
3319 |
_version_ |
1766393527677222912 |