Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei
Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa...
Published in: | Molecular Reproduction and Development |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/12227 https://doi.org/10.1002/mrd.22120 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/12227 2023-05-15T18:15:44+02:00 Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei Lin, Fan Xu, Shihong Ma, Daoyuan Xiao, Zhizhong Zhao, Chunyan Xiao, Yongshuang Chi, Liang Liu, Qinghua Li, Jun Liu, QH (reprint author), Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. 2012-11-01 http://ir.qdio.ac.cn/handle/337002/12227 https://doi.org/10.1002/mrd.22120 英语 eng MOLECULAR REPRODUCTION AND DEVELOPMENT Lin, Fan; Xu, Shihong; Ma, Daoyuan; Xiao, Zhizhong; Zhao, Chunyan; Xiao, Yongshuang; Chi, Liang; Liu, Qinghua; Li, Jun.Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei,MOLECULAR REPRODUCTION AND DEVELOPMENT,2012,79(11):803-813 http://ir.qdio.ac.cn/handle/337002/12227 doi:10.1002/mrd.22120 Differential Expression Rainbow-trout Teleost Fish Cell Lineage Zebrafish Rna Generation Oogenesis Embryos Cloning Biochemistry & Molecular Biology Cell Biology Developmental Biology Reproductive Biology Article 期刊论文 2012 ftchinacasciocas https://doi.org/10.1002/mrd.22120 2022-06-27T05:34:23Z Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa cDNA (Smvas) from turbot (Scophthalmus maximus), a marine Euteleostei species, and investigated its expression patterns by RT-PCR and in situ hybridization during embryogenesis and gametogenesis to identify the germ cell lineage in this species. The deduced amino acid sequence of the isolated cDNA shared typical characteristics of Vasa protein and high identity to Vasa homologues in medaka (76.9%) and zebrafish (68.5%). The Smvas transcripts were exclusively detected in germ cells of testis and ovary, and exhibited an interesting dynamic localization pattern during oogenesis. The distribution pattern of Smvas during embyogenesis in this Euteleostei closely resembled the pattern observed in zebrafish (belonging to Osteriophysans) rather than medaka (belonging to Euteleostei). Thus, it is concluded that Smvas isolated in this study is a germ cell specific molecular marker in turbot. Furthermore, we hypothesize that Euteleostei could localize vasa mRNA by a special mode. The results not only facilitate the germ cell manipulation of the turbot, but also improve our understanding of germline development and evolution of vasa localization in teleost. Mol. Reprod. Dev. 79: 803813, 2012. (C) 2012 Wiley Periodicals, Inc. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Vasa ENVELOPE(25.177,25.177,67.587,67.587) Molecular Reproduction and Development 79 11 803 813 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Differential Expression Rainbow-trout Teleost Fish Cell Lineage Zebrafish Rna Generation Oogenesis Embryos Cloning Biochemistry & Molecular Biology Cell Biology Developmental Biology Reproductive Biology |
spellingShingle |
Differential Expression Rainbow-trout Teleost Fish Cell Lineage Zebrafish Rna Generation Oogenesis Embryos Cloning Biochemistry & Molecular Biology Cell Biology Developmental Biology Reproductive Biology Lin, Fan Xu, Shihong Ma, Daoyuan Xiao, Zhizhong Zhao, Chunyan Xiao, Yongshuang Chi, Liang Liu, Qinghua Li, Jun Liu, QH (reprint author), Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei |
topic_facet |
Differential Expression Rainbow-trout Teleost Fish Cell Lineage Zebrafish Rna Generation Oogenesis Embryos Cloning Biochemistry & Molecular Biology Cell Biology Developmental Biology Reproductive Biology |
description |
Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa cDNA (Smvas) from turbot (Scophthalmus maximus), a marine Euteleostei species, and investigated its expression patterns by RT-PCR and in situ hybridization during embryogenesis and gametogenesis to identify the germ cell lineage in this species. The deduced amino acid sequence of the isolated cDNA shared typical characteristics of Vasa protein and high identity to Vasa homologues in medaka (76.9%) and zebrafish (68.5%). The Smvas transcripts were exclusively detected in germ cells of testis and ovary, and exhibited an interesting dynamic localization pattern during oogenesis. The distribution pattern of Smvas during embyogenesis in this Euteleostei closely resembled the pattern observed in zebrafish (belonging to Osteriophysans) rather than medaka (belonging to Euteleostei). Thus, it is concluded that Smvas isolated in this study is a germ cell specific molecular marker in turbot. Furthermore, we hypothesize that Euteleostei could localize vasa mRNA by a special mode. The results not only facilitate the germ cell manipulation of the turbot, but also improve our understanding of germline development and evolution of vasa localization in teleost. Mol. Reprod. Dev. 79: 803813, 2012. (C) 2012 Wiley Periodicals, Inc. |
format |
Article in Journal/Newspaper |
author |
Lin, Fan Xu, Shihong Ma, Daoyuan Xiao, Zhizhong Zhao, Chunyan Xiao, Yongshuang Chi, Liang Liu, Qinghua Li, Jun Liu, QH (reprint author), Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_facet |
Lin, Fan Xu, Shihong Ma, Daoyuan Xiao, Zhizhong Zhao, Chunyan Xiao, Yongshuang Chi, Liang Liu, Qinghua Li, Jun Liu, QH (reprint author), Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_sort |
Lin, Fan |
title |
Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei |
title_short |
Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei |
title_full |
Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei |
title_fullStr |
Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei |
title_full_unstemmed |
Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei |
title_sort |
germ line specific expression of a vasa homologue gene in turbot (scophthalmus maximus): evidence for vasa localization at cleavage furrows in euteleostei |
publishDate |
2012 |
url |
http://ir.qdio.ac.cn/handle/337002/12227 https://doi.org/10.1002/mrd.22120 |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
MOLECULAR REPRODUCTION AND DEVELOPMENT Lin, Fan; Xu, Shihong; Ma, Daoyuan; Xiao, Zhizhong; Zhao, Chunyan; Xiao, Yongshuang; Chi, Liang; Liu, Qinghua; Li, Jun.Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei,MOLECULAR REPRODUCTION AND DEVELOPMENT,2012,79(11):803-813 http://ir.qdio.ac.cn/handle/337002/12227 doi:10.1002/mrd.22120 |
op_doi |
https://doi.org/10.1002/mrd.22120 |
container_title |
Molecular Reproduction and Development |
container_volume |
79 |
container_issue |
11 |
container_start_page |
803 |
op_container_end_page |
813 |
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1766188943058927616 |