The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection
Tissue factor pathway inhibitor 1 (TFPI-1) is a Kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. In teleosts, TFPI-1-like sequences have been found to exist in two species (Danio rerio and Cyprinus carpio); however, the potential function of f...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/11975 2023-05-15T18:06:09+02:00 The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection Zhang, Min Sun, Li 2011-03-01 http://ir.qdio.ac.cn/handle/337002/11976 https://doi.org/10.1016/j.dci.2010.10.006 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Zhang, Min; Sun, Li.The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(3):247-252 http://ir.qdio.ac.cn/handle/337002/11976 doi:10.1016/j.dci.2010.10.006 Tissue Factor Pathway Inhibitor 1 Sciaenops Ocellatus Antibacterial Serine Protease Inhibitor Immunology Zoology Article 期刊论文 2011 ftchinacasciocas https://doi.org/10.1016/j.dci.2010.10.006 2022-06-27T05:34:17Z Tissue factor pathway inhibitor 1 (TFPI-1) is a Kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. In teleosts, TFPI-1-like sequences have been found to exist in two species (Danio rerio and Cyprinus carpio); however, the potential function of fish TFPI-1 has not been investigated. In this study, we identified and analyzed a TFPI-1 homologue. SoTFPI-1, from red drum (Sciaenops ocellatus). The deduced amino acid sequence of SoTFPI-1 is 284 residues in length and contains three Kunitz domains, an acidic N-terminus, and a basic C-terminus. SoTFPI-1 shares 49.5% and 46.9% overall sequence identities with the TFPI-1 of D. rerio and C. carpi's,. respectively. Quantitative real time RT-PCR analysis showed that constitutive SoTFPI-1 expression occurred, in increasing order. in kidney, brain, liver, gill, blood, spleen, muscle, and heart. Bacterial infection and lipopolysaccharide exposure upregulated SoTFPI-1 expression in kidney in time-dependent manners. Recombinant SoTFPI-1 (rSoTFPI-1) purified from Escherichia coli exhibits not only serine protease inhibitor activity but also bactericidal activity in a manner that is independent of any host factors. A synthetic peptide, TO17, corresponding to the C-terminal basic region of SoTFPI-1 also possesses antibacterial effect that is more potent than that of the full-length rSoTFPI-1. Taken together, these results demonstrate that (i)SoTFPI-1 is a biologically active serine protease inhibitor endowed with bactericidal property; (ii) provide the first indication that teleost TFPI-1 is likely to be involved in anti-microbial infection and thus is linked to innate immune defense. (C) 2010 Elsevier Ltd. All rights reserved. Tissue factor pathway inhibitor 1 (TFPI-1) is a Kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. In teleosts, TFPI-1-like sequences have been found to exist in two species (Danio rerio and Cyprinus carpio); however, the potential function of fish ... Article in Journal/Newspaper Red drum Sciaenops ocellatus Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 35 3 247 252 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Tissue Factor Pathway Inhibitor 1 Sciaenops Ocellatus Antibacterial Serine Protease Inhibitor Immunology Zoology |
spellingShingle |
Tissue Factor Pathway Inhibitor 1 Sciaenops Ocellatus Antibacterial Serine Protease Inhibitor Immunology Zoology Zhang, Min Sun, Li The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection |
topic_facet |
Tissue Factor Pathway Inhibitor 1 Sciaenops Ocellatus Antibacterial Serine Protease Inhibitor Immunology Zoology |
description |
Tissue factor pathway inhibitor 1 (TFPI-1) is a Kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. In teleosts, TFPI-1-like sequences have been found to exist in two species (Danio rerio and Cyprinus carpio); however, the potential function of fish TFPI-1 has not been investigated. In this study, we identified and analyzed a TFPI-1 homologue. SoTFPI-1, from red drum (Sciaenops ocellatus). The deduced amino acid sequence of SoTFPI-1 is 284 residues in length and contains three Kunitz domains, an acidic N-terminus, and a basic C-terminus. SoTFPI-1 shares 49.5% and 46.9% overall sequence identities with the TFPI-1 of D. rerio and C. carpi's,. respectively. Quantitative real time RT-PCR analysis showed that constitutive SoTFPI-1 expression occurred, in increasing order. in kidney, brain, liver, gill, blood, spleen, muscle, and heart. Bacterial infection and lipopolysaccharide exposure upregulated SoTFPI-1 expression in kidney in time-dependent manners. Recombinant SoTFPI-1 (rSoTFPI-1) purified from Escherichia coli exhibits not only serine protease inhibitor activity but also bactericidal activity in a manner that is independent of any host factors. A synthetic peptide, TO17, corresponding to the C-terminal basic region of SoTFPI-1 also possesses antibacterial effect that is more potent than that of the full-length rSoTFPI-1. Taken together, these results demonstrate that (i)SoTFPI-1 is a biologically active serine protease inhibitor endowed with bactericidal property; (ii) provide the first indication that teleost TFPI-1 is likely to be involved in anti-microbial infection and thus is linked to innate immune defense. (C) 2010 Elsevier Ltd. All rights reserved. Tissue factor pathway inhibitor 1 (TFPI-1) is a Kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. In teleosts, TFPI-1-like sequences have been found to exist in two species (Danio rerio and Cyprinus carpio); however, the potential function of fish ... |
format |
Article in Journal/Newspaper |
author |
Zhang, Min Sun, Li |
author_facet |
Zhang, Min Sun, Li |
author_sort |
Zhang, Min |
title |
The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection |
title_short |
The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection |
title_full |
The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection |
title_fullStr |
The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection |
title_full_unstemmed |
The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection |
title_sort |
tissue factor pathway inhibitor 1 of sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection |
publishDate |
2011 |
url |
http://ir.qdio.ac.cn/handle/337002/11976 https://doi.org/10.1016/j.dci.2010.10.006 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Zhang, Min; Sun, Li.The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(3):247-252 http://ir.qdio.ac.cn/handle/337002/11976 doi:10.1016/j.dci.2010.10.006 |
op_doi |
https://doi.org/10.1016/j.dci.2010.10.006 |
container_title |
Developmental & Comparative Immunology |
container_volume |
35 |
container_issue |
3 |
container_start_page |
247 |
op_container_end_page |
252 |
_version_ |
1766177731088744448 |