The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection
The Rab family proteins belong to the Ras-like GTPase superfamily and play important roles in intracellular membrane trafficking. To date no studies on fish Rab have been documented, though rab-like sequences have been found in a number of teleosts. In this study, we identified and analyzed a Rab ho...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/11973 2023-05-15T18:06:06+02:00 The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection Hu, Yong-hua Deng, Tian Sun, Li 2011-12-01 http://ir.qdio.ac.cn/handle/337002/11974 https://doi.org/10.1016/j.fsi.2011.08.019 英语 eng FISH & SHELLFISH IMMUNOLOGY Hu, Yonghua; Deng, Tian; Sun, Li.The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection,FISH & SHELLFISH IMMUNOLOGY,2011,31(6):1005-1012 http://ir.qdio.ac.cn/handle/337002/11974 doi:10.1016/j.fsi.2011.08.019 Rab Gtpase Sciaenops Ocellatus Bacterial Infection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Article 期刊论文 2011 ftchinacasciocas https://doi.org/10.1016/j.fsi.2011.08.019 2022-06-27T05:34:17Z The Rab family proteins belong to the Ras-like GTPase superfamily and play important roles in intracellular membrane trafficking. To date no studies on fish Rab have been documented, though rab-like sequences have been found in a number of teleosts. In this study, we identified and analyzed a Rab homologue, SoRab1, from red drum, Sciaenops ocellatus. The cDNA of SoRab1 contains a 5'- untranslated region (UTR) of 358 bp, an open reading frame (ORF) of 612 bp, and a 3'-UTR of 265 bp. The ORF encodes a putative protein of 203 residues, which shares 92-99% overall sequence identities with the Rab1 from fish, human, and mouse. SoRab1 possesses a typical Rab1 GTPase domain with the conserved G box motifs and the switch I and switch II regions. Recombinant SoRab1 purified from Escherichia coli exhibits apparent GTPase activity. Quantitative real time RT-PCR analysis showed that SoRab1 expression was detected in a number of tissues, with the lowest expression found in blood and highest expression found in muscle. Bacterial and lipopolysaccharide challenges significantly upregulated SoRab1 expression in liver, kidney, and spleen in time-dependent manners. Transient overexpression of SoRab1 in primary hepatocytes reduced intracellular bacterial infection, whereas interference with SoRab1 expression by RNAi enhanced intracellular bacterial invasion. These results provide the first indication that a fish Rab1 GTPase, SoRab1, regulates intracellular bacterial infection and thus is likely to play a role in bacteria-induced host immune defense. (C) 2011 Elsevier Ltd. All rights reserved. The Rab family proteins belong to the Ras-like GTPase superfamily and play important roles in intracellular membrane trafficking. To date no studies on fish Rab have been documented, though rab-like sequences have been found in a number of teleosts. In this study, we identified and analyzed a Rab homologue, SoRab1, from red drum, Sciaenops ocellatus. The cDNA of SoRab1 contains a 5'- untranslated region (UTR) of 358 bp, an open reading frame ... Article in Journal/Newspaper Red drum Sciaenops ocellatus Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 31 6 1005 1012 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Rab Gtpase Sciaenops Ocellatus Bacterial Infection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Rab Gtpase Sciaenops Ocellatus Bacterial Infection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Hu, Yong-hua Deng, Tian Sun, Li The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection |
topic_facet |
Rab Gtpase Sciaenops Ocellatus Bacterial Infection Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
The Rab family proteins belong to the Ras-like GTPase superfamily and play important roles in intracellular membrane trafficking. To date no studies on fish Rab have been documented, though rab-like sequences have been found in a number of teleosts. In this study, we identified and analyzed a Rab homologue, SoRab1, from red drum, Sciaenops ocellatus. The cDNA of SoRab1 contains a 5'- untranslated region (UTR) of 358 bp, an open reading frame (ORF) of 612 bp, and a 3'-UTR of 265 bp. The ORF encodes a putative protein of 203 residues, which shares 92-99% overall sequence identities with the Rab1 from fish, human, and mouse. SoRab1 possesses a typical Rab1 GTPase domain with the conserved G box motifs and the switch I and switch II regions. Recombinant SoRab1 purified from Escherichia coli exhibits apparent GTPase activity. Quantitative real time RT-PCR analysis showed that SoRab1 expression was detected in a number of tissues, with the lowest expression found in blood and highest expression found in muscle. Bacterial and lipopolysaccharide challenges significantly upregulated SoRab1 expression in liver, kidney, and spleen in time-dependent manners. Transient overexpression of SoRab1 in primary hepatocytes reduced intracellular bacterial infection, whereas interference with SoRab1 expression by RNAi enhanced intracellular bacterial invasion. These results provide the first indication that a fish Rab1 GTPase, SoRab1, regulates intracellular bacterial infection and thus is likely to play a role in bacteria-induced host immune defense. (C) 2011 Elsevier Ltd. All rights reserved. The Rab family proteins belong to the Ras-like GTPase superfamily and play important roles in intracellular membrane trafficking. To date no studies on fish Rab have been documented, though rab-like sequences have been found in a number of teleosts. In this study, we identified and analyzed a Rab homologue, SoRab1, from red drum, Sciaenops ocellatus. The cDNA of SoRab1 contains a 5'- untranslated region (UTR) of 358 bp, an open reading frame ... |
format |
Article in Journal/Newspaper |
author |
Hu, Yong-hua Deng, Tian Sun, Li |
author_facet |
Hu, Yong-hua Deng, Tian Sun, Li |
author_sort |
Hu, Yong-hua |
title |
The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection |
title_short |
The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection |
title_full |
The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection |
title_fullStr |
The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection |
title_full_unstemmed |
The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection |
title_sort |
rab1 gtpase of sciaenops ocellatus modulates intracellular bacterial infection |
publishDate |
2011 |
url |
http://ir.qdio.ac.cn/handle/337002/11974 https://doi.org/10.1016/j.fsi.2011.08.019 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Hu, Yonghua; Deng, Tian; Sun, Li.The Rab1 GTPase of Sciaenops ocellatus modulates intracellular bacterial infection,FISH & SHELLFISH IMMUNOLOGY,2011,31(6):1005-1012 http://ir.qdio.ac.cn/handle/337002/11974 doi:10.1016/j.fsi.2011.08.019 |
op_doi |
https://doi.org/10.1016/j.fsi.2011.08.019 |
container_title |
Fish & Shellfish Immunology |
container_volume |
31 |
container_issue |
6 |
container_start_page |
1005 |
op_container_end_page |
1012 |
_version_ |
1766177675583422464 |