The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation
High mobility group box 1 protein (HMGB1) is a chromatin-associated nonhistone protein that is involved in nucleosome formation and transcriptional regulation. In addition, HMGB1 is also known as an extracellular cytokine that triggers inflammation and immune responses. HMGB1-like sequences have bee...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/11967 2023-05-15T18:06:09+02:00 The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation Zhao, Lu Hu, Yong-hua Sun, Jin-sheng Sun, Li 2011-10-01 http://ir.qdio.ac.cn/handle/337002/11968 https://doi.org/10.1016/j.dci.2011.03.025 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Zhao, Lu; Hu, Yonghua; Sun, Jinsheng; Sun, Li.The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(10):1050-1056 http://ir.qdio.ac.cn/handle/337002/11968 doi:10.1016/j.dci.2011.03.025 High Mobility Group Box Protein Sciaenops Ocellatus Cytokine Macrophage Immunology Zoology Article 期刊论文 2011 ftchinacasciocas https://doi.org/10.1016/j.dci.2011.03.025 2022-06-27T05:34:17Z High mobility group box 1 protein (HMGB1) is a chromatin-associated nonhistone protein that is involved in nucleosome formation and transcriptional regulation. In addition, HMGB1 is also known as an extracellular cytokine that triggers inflammation and immune responses. HMGB1-like sequences have been identified in a number of fish species, however, the function of piscine HMGB1 remains uninvestigated. In this study, we reported the identification and analysis of SoHMGB1, an HMGB1 homologue from red drum (Sciaenops ocellatus). SoHMGB1 is 206 residues in length and contains two basic HMG boxes and a highly acidic C-terminal domain. SoHMGB1 shares 71-87% overall sequence identities with the HMGB1 counterparts from human, rat, and several fish species. Quantitative real time RT-PCR analysis showed that constitutive SoHMGB1 expression was detected in various tissues, with the lowest and highest levels found in kidney and muscle respectively. Bacterial challenge upregulated SoHMGB1 expression in head kidney (HK) and HK macrophages and induced extracellular secretion of SoHMGB1 by the activated macrophages. Recombinant SoHMGB1 (rSoHMGB1) purified from yeast exhibited no direct antimicrobial effect but was significantly stimulatory on the proliferation, activation, and bactericidal activity of HK macrophages. Taken together, these results indicate for the first time that a fish HMGB1. SoHMGB1, can function as a secreted cytokine in the event of bacterial infection and promote innate defense through the activation of macrophages. (C) 2011 Elsevier Ltd. All rights reserved. High mobility group box 1 protein (HMGB1) is a chromatin-associated nonhistone protein that is involved in nucleosome formation and transcriptional regulation. In addition, HMGB1 is also known as an extracellular cytokine that triggers inflammation and immune responses. HMGB1-like sequences have been identified in a number of fish species, however, the function of piscine HMGB1 remains uninvestigated. In this study, we reported the identification and ... Article in Journal/Newspaper Red drum Sciaenops ocellatus Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 35 10 1052 1058 |
institution |
Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
High Mobility Group Box Protein Sciaenops Ocellatus Cytokine Macrophage Immunology Zoology |
spellingShingle |
High Mobility Group Box Protein Sciaenops Ocellatus Cytokine Macrophage Immunology Zoology Zhao, Lu Hu, Yong-hua Sun, Jin-sheng Sun, Li The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation |
topic_facet |
High Mobility Group Box Protein Sciaenops Ocellatus Cytokine Macrophage Immunology Zoology |
description |
High mobility group box 1 protein (HMGB1) is a chromatin-associated nonhistone protein that is involved in nucleosome formation and transcriptional regulation. In addition, HMGB1 is also known as an extracellular cytokine that triggers inflammation and immune responses. HMGB1-like sequences have been identified in a number of fish species, however, the function of piscine HMGB1 remains uninvestigated. In this study, we reported the identification and analysis of SoHMGB1, an HMGB1 homologue from red drum (Sciaenops ocellatus). SoHMGB1 is 206 residues in length and contains two basic HMG boxes and a highly acidic C-terminal domain. SoHMGB1 shares 71-87% overall sequence identities with the HMGB1 counterparts from human, rat, and several fish species. Quantitative real time RT-PCR analysis showed that constitutive SoHMGB1 expression was detected in various tissues, with the lowest and highest levels found in kidney and muscle respectively. Bacterial challenge upregulated SoHMGB1 expression in head kidney (HK) and HK macrophages and induced extracellular secretion of SoHMGB1 by the activated macrophages. Recombinant SoHMGB1 (rSoHMGB1) purified from yeast exhibited no direct antimicrobial effect but was significantly stimulatory on the proliferation, activation, and bactericidal activity of HK macrophages. Taken together, these results indicate for the first time that a fish HMGB1. SoHMGB1, can function as a secreted cytokine in the event of bacterial infection and promote innate defense through the activation of macrophages. (C) 2011 Elsevier Ltd. All rights reserved. High mobility group box 1 protein (HMGB1) is a chromatin-associated nonhistone protein that is involved in nucleosome formation and transcriptional regulation. In addition, HMGB1 is also known as an extracellular cytokine that triggers inflammation and immune responses. HMGB1-like sequences have been identified in a number of fish species, however, the function of piscine HMGB1 remains uninvestigated. In this study, we reported the identification and ... |
format |
Article in Journal/Newspaper |
author |
Zhao, Lu Hu, Yong-hua Sun, Jin-sheng Sun, Li |
author_facet |
Zhao, Lu Hu, Yong-hua Sun, Jin-sheng Sun, Li |
author_sort |
Zhao, Lu |
title |
The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation |
title_short |
The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation |
title_full |
The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation |
title_fullStr |
The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation |
title_full_unstemmed |
The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation |
title_sort |
high mobility group box 1 protein of sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation |
publishDate |
2011 |
url |
http://ir.qdio.ac.cn/handle/337002/11968 https://doi.org/10.1016/j.dci.2011.03.025 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Zhao, Lu; Hu, Yonghua; Sun, Jinsheng; Sun, Li.The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(10):1050-1056 http://ir.qdio.ac.cn/handle/337002/11968 doi:10.1016/j.dci.2011.03.025 |
op_doi |
https://doi.org/10.1016/j.dci.2011.03.025 |
container_title |
Developmental & Comparative Immunology |
container_volume |
35 |
container_issue |
10 |
container_start_page |
1052 |
op_container_end_page |
1058 |
_version_ |
1766177726893391872 |